Pseudomonas Aeruginosa and Newer β-Lactamases: An Emerging Resistance Threat

Basak, Silpi; Attal, Ruchita; Rajurkar, Monali N.

doi:10.5772/34743

Cited by 8 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The highest frequency of class C β-lactamases genes in P. aeruginosa were isolated from blood followed by urine, ear swab, wound swab and sputum. These results agree with the study in India reported by Basak et al (2012) 48 in that the highest percentage of class C β-lactamase genes in P. aeruginosa were found in blood.…”

Section: Discussionsupporting

confidence: 93%

“…P. aeruginosa isolated from blood showed the highest production of bla TEM , bla SHV , bla CTXM-1 , bla VEB and bla OXA-1 genes production followed by ear swab, urine, sputum and wound swab. This result agrees with a study in India reported by Basak et al (2012) 48 where the highest β-lactamases producing P. aeruginosa isolates were from blood, but disagrees with Azizi et al (2015) 49 in Iran who found that highest production was in P. aeruginosa isolated from wound followed by urine, sputum and blood. There was insignificant association between the presence of class C β-lactamase genes and site of infection (P=0.215) found in the present study.…”

Section: Discussionsupporting

confidence: 79%

See 1 more Smart Citation

β-lactamases (bla TEM, bla SHV, bla CTXM-1, bla VEB, bla OXA-1) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

et al. 2020

View full text Add to dashboard Cite

Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases gene (extended spectrum β-lactamases (ESBLs) genes and class C β-lactamases genes) frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross-sectional study conducted in Khartoum State. A total of 80 isolates were confirmed as Ps. aeruginosa through conventional identification methods and species-specific primers (the remaining 40 isolates were other bacterial species). The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase genes (blaTEM, blaSHV, blaCTXM-1, blaVEB, blaOXA-1, blaAmpC and blaDHA). Results: Of the 80 confirmed Ps. aeruginosa isolates, 8 (10%) were resistant to Imipenem while all isolates were resistant to Amoxicillin and Amoxyclav (100%). A total of 43 (54%) Ps. aeruginosa isolates were positive for ESBLs genes, while 27 (34%) were positive for class C β-lactamases, and 20 (25%) were positive for both classes. Frequency of ESBLs genes was as follows: blaTEM, 19 (44.2%); blaSHV, 16 (37.2%); blaCTX-M1, 10 (23.3%); blaVEB, 14 (32.6%); and blaOXA-1, 7 (16.3%). Occurrence of class C β-lactamases genes was blaAmpC 22 (81.5%) and blaDHA 8 (29.6%). In total, 3 (11.1%) isolates were positive for both blaAmpC and blaDHA genes. Conclusion: Ps. aeruginosa isolates showed a high rate of β-lactamases production, with co-resistance to other antibiotic classes. The lowest resistance rate of Ps. aeruginosa was to Imipenem followed by Gentamicin and Ciprofloxacin. No statistically significant relationship between production of β-lactamases in Ps. aeruginosa and resistance to third generation cephalosporins was found.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 79%

β-lactamases (bla TEM, bla SHV, bla CTXM-1, bla VEB, bla OXA-1) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Aggarwal et al, (9) and Shaikh et al, (10) on similar study observed prevalence rate of 20.3% and 25.13% which is comparable to our findings. In contrast, high prevalence of ESBL producing Pseudomonas aeruginosa was also reported by Vijay Mane et al, Varun Goel et al, and Silpi Bask et al who observed 57%, 42.3% and 40% respectively (11,12,13) . This may be due to the fact that prevalence in any geographical area is influenced by the antibiotic policy of that place, the carriage rate among the hospital personnel and type of disinfectant used.…”

Section: Discussionmentioning

confidence: 57%

ESBL producing Pseudomonas aeruginosa in clinical specimens: Is it a scary nightmare or paper tiger?

Nithyalakshmi¹,

Vidhyarani²,

Mohanakrishnan³

et al. 2016

Ind. Jour. of Microb. Res.

View full text Add to dashboard Cite

Background: Pseudomonas aeruginosa well known as opportunistic pathogen, has been implicated in life threatening nosocomial infections in recent years. This can be attributable to acquired resistance by plasmid mediated different types of extended spectrum beta lactamases(ESBL). The present study was undertaken to detect the ESBL production of Pseudomonas aeruginosastrains and analyze their susceptibility pattern. Methods: Various clinical specimens received in our laboratory were processed and Pseudomonas aeruginosa was identified as per standard microbiological procedure. A total of 132 clinical isolates of Pseudomonas aeruginosa identified during the study period were included in the study. All isolates were subjected for ESBL screening test. Potential ESBL producer was then subjected for ESBL Phenotypic confirmatory test-Disc Diffusion method(PCDDT). Antimicrobial susceptibility test was performed by Kirby-Bauer disc diffusion method on all confirmed isolates by PCDDT method as per Clinical Laboratory Standard Institute (CLSI 2016) guidelines. Results: By phenotypic confirmatory method, the frequency of occurrence of ESBL among the isolates identified was 21.96% (n = 29).The highest number of ESBL producers was obtained from urine samples(27.7%) followed by respiratory infection (23.68%) and wound infection (22.95%). In-vitro susceptibility of ESBL producers revealed high level of resistance to third generation cephalosporin. Low resistance was observed to Imipenem (3.4%) and Piperacillin-Tazobactam (13.79%). Conclusion: Our study highlights the unique challenge imposed by Pseudomonas aeruginosa as the therapeutic choices are limited by ESBL production. To overcome this issue, we recommend routine ESBL detection and surveillance of antibiotic resistance in hospital settings.

show abstract

“…From 2008 to 2010, out of total 250 Pseudomonas aeruginosa strains studied 40%, 42% and 11.2% were ESBL, AmpC β -lactamases and MBL producers respectively [40]. Amongst these ESBL and AmpC β -lactamase producers 27.2% P.aeruginosa strains produced both ESBL as well as AmpC β -lactamases.…”

Section: Scenario In Our Hospital In Last 5 Yearsmentioning

confidence: 98%