Pseudomonas aeruginosa and Pseudomonas putida outbreak associated with contaminated water outlets in an oncohaematology paediatric unit

Molecular epidemiological investigation of an outbreak of Pseudomonas aeruginosa infection in an SCT unit

“…12,13 In the SCT unit of Careggi hospital, over a 4-month period, an outbreak of P. aeruginosa, constituted by six episodes of severe sepsis, was detected; four of these sepses involved patients who followed each other into room N of the ward. AFLP analysis of the isolates from the six patients established definitively the clonality of the four strains from patients in room N and, at the same time, the independent origin of the other two P. aeruginosa isolates.…”

Post-Outbreak Investigation of Pseudomonas aeruginosa Faucet Contamination by Quantitative Polymerase Chain Reaction and Environmental Factors Affecting Positivity

“…1 Several of these outbreaks have been directly or indirectly linked to water distribution systems. [2][3][4][5][6][7][8][9][10][11][12][13][14] In ICUs, 30%-50% of P. aeruginosa infections have been associated with water. 15 A multicentric prospective study recently established tap contamination in patient rooms as an important environmental risk factor for P. aeruginosa acquisition.…”

“…3,20 Once contaminated, eradication of P. aeruginosa in the water system is challenging and often results in replacing related devices 5,[7][8][9][10][11][12]14 or installing point-of-use 0.2-μm filters. 4,13,14 Although cultivation is the reference method, it may not reveal background contamination that may flare up when the environment becomes favorable for growth and culturability. Environmental stressors present in water such as chlorine and copper decrease culturability without necessarily decreasing viability.…”

The Complete Nucleotide Sequence of the Carbapenem Resistance-Conferring Conjugative Plasmid pLD209 from a Pseudomonas putida Clinical Strain Reveals a Chimeric Design Formed by Modules Derived from Both Environmental and Clinical Bacteria

“…seudomonas putida is an opportunistic pathogen generally susceptible to most clinically employed antimicrobial agents and ubiquitous in several niches, including soil, freshwater, and animal surfaces (1). Recently, however, VIM-and IMP-like metallo-␤-lactamase (M␤L)-containing P. putida clinical strains displaying resistance to most ␤-lactams have been described and proposed to represent M␤L gene reservoirs (2)(3)(4)(5).…”