Pseudomonas aeruginosa cytochrome P450 CYP168A1 is a fatty acid hydroxylase that metabolizes arachidonic acid to the vasodilator 19-HETE

Abstract

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Effect of feeding a dried distillers’ grains with solubles diet on the metabolism of the intestinal wall in Guanling crossbred cattle: a preliminary assessment

“…Among them, 9-OxoODE and 9,10,13-TriHOME are pro-inflammatory and are produced through lipoxygenase-catalyzed linoleic acid metabolism, which can influence the production of inflammatory mediators, such as pro-inflammatory cytokines and leukocyte chemokines ( 42 , 43 ). Arachidonic acid is a polyunsaturated fatty acid, which is metabolized mainly through the tapping pathway enzymes that catalyze cyclooxygenase and lipoxygenase into metabolites with a variety of biological activities; these metabolites are involved in the genesis and regulation of intestinal inflammation, and reducing their production helps to inhibit the inflammatory process ( 44 , 45 ). Our study demonstrated that DDGS diets were able to attenuate intestinal inflammation and reduce inflammatory mediators by downregulating the concentrations of 9-OxoODE, 9,10,13-TriHOME, and arachidonic acid, thereby reducing the severity of the inflammatory response.…”

A Novel Approach To Identify Inhibitors of Iron Acquisition Systems of Pseudomonas aeruginosa

“…It is well known that the primary mode of action of azole drugs such as econazole is mediated by binding the Hm cofactor ( 37 ) to inactivate cytochrome P450 enzymes, which are essential for diverse catalytic roles ( 38 ). Since P. aeruginosa has genes that encode P450 enzymes ( 39 ) and because it has been shown that econazole can bind these P. aeruginosa P450 enzymes ( 40 ), it is possible that econazole could inactivate the P. aeruginosa P450 enzymes and inhibit growth. If this was the case, we would expect to also observe the antipseudomonal activity of econazole in the presence of FeCl 3 or FeSO 4 and not just specifically in the presence of Hm ( Fig.…”