Pseudomonas aeruginosa L10: A Hydrocarbon-Degrading, Biosurfactant-Producing, and Plant-Growth-Promoting Endophytic Bacterium Isolated From a Reed (Phragmites australis)

Wu, Tao; Xu, Jie; Xie, Wenjun; Yao, Zhigang; Yang, Hongjun; Sun, Chunlong; Li, Xiaobin

doi:10.3389/fmicb.2018.01087

Cited by 91 publications

(70 citation statements)

References 59 publications

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“…Endophytic Serratia marcescens strain MSRBB2 was reported to produce prodiginines and serratamolides and was evaluated on it biosynthetic pathway with biochemical analysis of feeding experiments (Eckelmann et al, 2018). Prodiginines produced naturally by microorganisms are group of tripyrrole alkaloid which has wide bioactivity, including antibacterial, antiprotozoal, and antifungi (You et al, 2019). This also indicated that the antibacterial activity of bacterial endophytes from mangrove leaves in this study may due to the production of secondary metabolites such as prodiginines.…”

Section: Discussionsupporting

confidence: 56%

“…Pseudomonas also has great potential on producing antibiotic bioactive potential such as indole derivatives, phenazines, pyrrolnitrin-type antibiotics, glycolipids, another substances (Pratiwi et al, 2017). Wu et al (2018) evaluated P. aeruginosa L10 as biosurfactant producing and excellent hydrocarbondegrading bacteria isolated from roots, stems and leaves of a Reed Phragmites australis).…”

Section: Discussionmentioning

confidence: 99%

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Bacterial endophytes from mangrove leaves with antibacterial and enzymatic activities

Pramono¹,

Irawan²,

Firdaus³

et al. 2019

MJM

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The aim of this study was to screen bacterial endophytes with antibacterial and enzyme activity from mangrove leaves of Indonesia. Methodology and results: Bacterial endophytes were isolated and evaluated for antibacterial activity against five strains of pathogenic bacteria. Enzymatic Index (EI) was measured to evaluate the production of protease, amylase and cellulase. Hemolysin test was performed on Blood Agar and the sensitivity to antibiotic was performed. Bacterial endophyte Strain 1-1 isolated from Bruguiera gymnorrhiza showed strong inhibition against Escherichia coli, Salmonella sp., Staphylococcus aureus and Pseudomonas aeruginosa with inhibition zone of 12.6 ± 1.4, 8.8 ± 4.1, 12.5 ± 2.3 and 8.4 ± 0.9 mm respectively. Isolate 1-16 which isolated from B. gymnorrhiza exhibited antibacterial activity against E. coli and P. aeruginosa, while Isolate 6-10 isolated from Avicennia lanata exhibited strong inhibition on Salmonella sp. (13.1 ± 3.3 mm). All of those three isolates produced protease, non-haemolysin-producing strain and sensitive to gentamicin or kanamycin but resistant to ampicillin, tetracycline and chloramphenicol. Those three isolates were identified based on homology of 16S rDNA sequence. Strain 1-1 and 1-16 were identified as P. aeruginosa, while strain 6-10 identified as S. marcescens. Conclusion, significance and impact of study: This finding was showed the potential endophytic bacteria from Indonesian mangrove plants with antibacterial and enzyme production.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Bacterial endophytes from mangrove leaves with antibacterial and enzymatic activities

Pramono¹,

Irawan²,

Firdaus³

et al. 2019

MJM

View full text Add to dashboard Cite

show abstract

“…Under the presence of compatible microbes in the plant system, fine-tuning takes place in the hormonal balance that leads to better plant growth, development, and reproduction even under pathogenic stresses [38,39,42,43]. Among rhizospheric and endophytic plant growth-promoting bacteria, Pseudomonas aeruginosa is the most studied and successful plant symbiont conferring resistance against a wider range of plant pathogens by direct antagonism or by triggering systemic resistance [44,45]. P. aeruginosa has adapted to a wide ecosystem and plays a major role in plant health [46][47][48].…”

Section: Introductionmentioning

confidence: 99%

“…It colonizes rhizospheric soil, roots, and in some cases aerial parts of the plant. It can even grow as endophytes [45,[47][48][49]. P. aeruginosa is an excellent endo-symbiont and has myco-parasitic ability, protecting plants directly from pathogen attack, and it is well known for its ability to stimulate plant growth and development [48][49][50].…”

Section: Introductionmentioning

confidence: 99%

Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.)

Singh

Malviya

et al. 2020

IJERPH

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Plant growth promoting rhizobacteria Pseudomonas aeruginosa strain MF-30 isolated from maize rhizosphere was characterized for several plant growth stimulating attributes. The strain MF-30 was also evaluated for antifungal properties against Rhizoctonia solani causing banded leaf and sheath blight in maize (Zea mays L.) under in vitro conditions and was found to have higher mycelial growth suppression in the culture suspension (67.41%) followed by volatile organic compounds (62.66%) and crude extract (51.20%) in a dual plate assay. The endophytic and epiphytic colonization ability was tested using Green Fluorescent Protein (GFP)-tagging. Visualization through confocal scanning laser microscope clearly indicated that strain MF-30 colonizes the root and foliar parts of the plants. Further, the effects of seed bio-priming with P. aeruginosa MF-30 was evaluated in the induction and bioaccumulation of defense-related biomolecules, enzymes, natural antioxidants, and other changes in maize under pot trial. This not only provided protection from R. solani but also ensured growth promotion under pathogenic stress conditions in maize. The maximum concentration of hydrogen peroxide (H2O2) was reported in the root and shoot of the plants treated with R. solani alone (8.47 and 17.50 mmol mg−1 protein, respectively) compared to bioagent, P. aeruginosa MF-30 bio-primed plants (3.49 and 7.50 mmol mg−1 protein, respectively). Effects on total soluble sugar content, total protein, and total proline were also found to enhanced significantly due to inoculation of P. aeruginosa MF-30. The activities of anti-oxidative defense enzymes phenylalanine ammonia lyase (PAL), ascorbate peroxidase, peroxidase, superoxide dismutase, and catalase increased significantly in the plants bio-primed with P. aeruginosa MF-30 and subsequent foliar spray of culture suspension of MF-30 compared to pathogen alone inoculated plants. qRT-PCR analysis revealed that seed bio-priming and foliar application of P. aeruginosa MF-30 significantly increased the expression of PR-1 and PR-10 genes with the simultaneous decrease in the disease severity and lesion length in the maize plants under pathogenic stress conditions. A significant enhancement of shoot and root biomass was recorded in MF-30 bio-primed plants as compared to untreated control (p < 0.05). Significant increase in plant growth and antioxidant content, as well as decreased disease severity in the P. aeruginosa MF-30 bio-primed plants, suggested the possibility of an eco-friendly and economical means of achieving antioxidants-rich, healthier maize plants.

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“…Moreover, they identified genes related to PHs degradation, such as putative genes encoding monooxygenase, dioxygenase, alcohol dehydrogenase, and aldehyde dehydrogenase. Genome annotation revealed that P. aeruginosa L10 contained a gene cluster involving in the biosynthesis of rhamnolipids, rhlABRI, which could be responsible for the observed biosurfactant activity [69].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Petroleum Hydrocarbons Degradation by Klebsiella pneumoniae and Pseudomonas aeruginosa

et al. 2018

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: The aim of this work was to develop bacterial communities to effectively degrade petroleum hydrocarbons (PHs). We investigated the biotic and abiotic contributors to differences in PHs degradation efficacy between two bacterial strains, Klebsiella pneumoniae (Kp) and Pseudomonas aeruginosa (Pa), screened out from the activated sludge of a petroleum refinery. We characterized the temporal variations in degradation efficacy for diesel and its five major constituents as a sole carbon source and identified more constituents they degraded. The growth characteristics, surface tension, hydrophobicity and emulsifiability of these two strains were measured. We further estimated the relationships between their degradation efficacy and all the biotic and abiotic factors. Results showed that the Pa strain had higher diesel degradation efficacy (58% on Day 14) and utilized more diesel constituents (86%) compared to Kp. Additionally, the growth of the Pa strain in diesel medium was faster than that of the Kp strain. The Pa strain had a lower surface tension and higher hydrophobicity and emulsifiability than Kp, while the surfactant produced by Pa was identified as rhamnolipids. Degradation of PHs was positively related to bacterial growth, hydrophobicity and emulsification but negatively related to surface tension. Overall, differences in degrading capacity for diesel constituents, relative growth rate, and biosurfactant production contributed to the variation in the PHs degradation efficacy of these two bacterial strains.

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Pseudomonas aeruginosa L10: A Hydrocarbon-Degrading, Biosurfactant-Producing, and Plant-Growth-Promoting Endophytic Bacterium Isolated From a Reed (Phragmites australis)

Cited by 91 publications

References 59 publications

Bacterial endophytes from mangrove leaves with antibacterial and enzymatic activities

Bacterial endophytes from mangrove leaves with antibacterial and enzymatic activities

Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.)

Comparison of Petroleum Hydrocarbons Degradation by Klebsiella pneumoniae and Pseudomonas aeruginosa

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