Pseudomonas aeruginosa produces aspirin insensitive eicosanoids and contributes to the eicosanoid profile of polymicrobial biofilms with Candida albicans

Fourie, Ruan; Ells, Ruan; Kemp, Gabré; Sebolai, Olihile M.; Albertyn, Jacobus; Pohl, Carolina H.

doi:10.1016/j.plefa.2017.01.008

Cited by 15 publications

(11 citation statements)

References 57 publications

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“…Extracellular vesicles thus can serve as a site of oxylipin biosynthesis and a vehicle in shuffling the enzymes, substrates, and products between the pathogen and the host. Additionally, roles for microbial oxylipins in microbial confrontations should also be explored more thoroughly in the context of the human microbiome (Fourie et al, ; Krause, Geginat, & Tammer, ).…”

Section: Discussionmentioning

confidence: 99%

Co‐opting oxylipin signals in microbial disease

Niu

Keller

2019

Cellular Microbiology

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Oxylipins, or oxygenated lipids, are universal signalling molecules across all kingdoms of life. These molecules, either produced by microbial pathogens or their mammalian host, regulate inflammation during microbial infection. In this review, we summarise current literature on the biosynthesis pathways of microbial oxylipins and their biological activity towards mammalian cells. Collectively, these studies have illustrated how microbial pathogens can modulate immune rsponse and disease outcome via oxylipin-mediated mechanisms.

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Section: Discussionmentioning

confidence: 99%

Co‐opting oxylipin signals in microbial disease

Niu

Keller

2019

Cellular Microbiology

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“…After incubation, biofilms were washed twice with sterile PBS, scraped off and suspended in PBS. Biofilms were then vortexed 3 times for 1 minute to remove adherent cells from one another (adapted from Fourie et al, 2017 ). For quantification of C. albicans , serially diluted cells were plated onto YM medium acidified with tartaric acid (final concentration 0.08%).…”

Section: Methodsmentioning

confidence: 99%

Candida albicans SET3 Plays a Role in Early Biofilm Formation, Interaction With Pseudomonas aeruginosa and Virulence in Caenorhabditis elegans

Fourie

Albertyn

Sebolai

et al. 2021

Front. Cell. Infect. Microbiol.

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The yeast Candida albicans exhibits multiple morphologies dependent on environmental cues. Candida albicans biofilms are frequently polymicrobial, enabling interspecies interaction through proximity and contact. The interaction between C. albicans and the bacterium, Pseudomonas aeruginosa, is antagonistic in vitro, with P. aeruginosa repressing the yeast-to-hyphal switch in C. albicans. Previous transcriptional analysis of C. albicans in polymicrobial biofilms with P. aeruginosa revealed upregulation of genes involved in regulation of morphology and biofilm formation, including SET3, a component of the Set3/Hos2 histone deacetylase complex (Set3C). This prompted the question regarding the involvement of SET3 in the interaction between C. albicans and P. aeruginosa, both in vitro and in vivo. We found that SET3 may influence early biofilm formation by C. albicans and the interaction between C. albicans and P. aeruginosa. In addition, although deletion of SET3 did not alter the morphology of C. albicans in the presence of P. aeruginosa, it did cause a reduction in virulence in a Caenorhabditis elegans infection model, even in the presence of P. aeruginosa.

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“…One of the best-studied Candida lipid metabolites is the eicosanoid, prostaglandin E 2 (PGE 2 ), which has been reported in C. albicans , C. dubliniensis , C. glabrata , C. parapsilosis and C. tropicalis . P. aeruginosa also secretes an array of fatty acid metabolites, including hydroxy fatty acids [71,72,73] as well as PGE 2 and prostaglandin F 2α [74]. Martínez and coworkers (2019) studied the effect of hydroxy fatty acids produced by the action of diol synthase in P. aeruginosa and found that the products, 10-hydroxy-octadecenoic acid (10-HOME) and 7,10-dihydroxy-octadecenoic acid (7,10-DiHOME), caused transcriptomic and proteomic alterations in P. aeruginosa [39,71].…”

Section: In Vitro Interaction Between Pseudomonas Aeruginosa and Cmentioning

confidence: 99%

“…Interestingly, 7,10-DiHOME has an inhibitory effect on the growth of C. albicans [75], while another hydroxy fatty acid, 3-hydroxy tetradecadienoic acid (3-OH 14:2), which is an endogenous linoleic acid metabolite of C. albicans , stimulates germ tube formation, even in the presence of farnesol [40]. Fourie and co-workers (2017) investigated the influence of co-incubation of C. albicans and P. aeruginosa on eicosanoid production from exogenous arachidonic acid by mixed species biofilms [74]. They found that, although co-incubation decreased the ability of individual colony forming units (CFUs) in the biofilm to produce eicosanoids, the final concentrations of all the studied eicosanoids (i.e., PGE 2 , PGF 2α , and 15-hydroxy eicosatetraenoic acid) increased compared to single species biofilms.…”

Section: In Vitro Interaction Between Pseudomonas Aeruginosa and Cmentioning

confidence: 99%

Beyond Antagonism: The Interaction Between Candida Species and Pseudomonas aeruginosa

Fourie

Pohl

2019

JoF

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There are many examples of the interaction between prokaryotes and eukaryotes. One such example is the polymicrobial colonization/infection by the various opportunistic pathogenic yeasts belonging to the genus Candida and the ubiquitous bacterium, Pseudomonas aeruginosa. Although this interaction has simplistically been characterized as antagonistic to the yeast, this review highlights the complexity of the interaction with various factors influencing both microbes. The first section deals with the interactions in vitro, looking specifically at the role of cell wall components, quorum sensing molecules, phenazines, fatty acid metabolites and competition for iron in the interaction. The second part of this review places all these interactions in the context of various infection or colonization sites, i.e., lungs, wounds, and the gastrointestinal tract. Here we see that the role of the host, as well as the methodology used to establish co-infection, are important factors, influencing the outcome of the disease. Suggested future perspectives for the study of this interaction include determining the influence of newly identified participants of the QS network of P. aeruginosa, oxylipin production by both species, as well as the genetic and phenotypic plasticity of these microbes, on the interaction and outcome of co-infection.

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Pseudomonas aeruginosa produces aspirin insensitive eicosanoids and contributes to the eicosanoid profile of polymicrobial biofilms with Candida albicans

Cited by 15 publications

References 57 publications

Co‐opting oxylipin signals in microbial disease

Co‐opting oxylipin signals in microbial disease

Candida albicans SET3 Plays a Role in Early Biofilm Formation, Interaction With Pseudomonas aeruginosa and Virulence in Caenorhabditis elegans

Beyond Antagonism: The Interaction Between Candida Species and Pseudomonas aeruginosa

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