Pseudomonas kuykendallii sp. nov.: A Novel γ-Proteobacteria Isolated From a Hexazinone Degrading Bioreactor

Abstract

Three strains of Gram-negative bacteria designated strains H2(T), H6, and H7 were isolated from bioreactors that degraded the herbicide hexazinone. Similar morphological characteristics, cellular fatty acid profiles, and 16S rRNA gene sequences show that the isolates are members of the same species. These characteristics also show that the isolates belong to the genus Pseudomonas with P. graminis, P. putida, and P. stutzeri as close relatives. The 16S rRNA gene of the H2(T) strain differed from that of type strains for P. graminis, P. putida, and P. stutzeri by 1.9, 2.5, and 2.7 %, respectively, indicating that the H2(T), H6, and H7 strains are related to P. graminis, P. putida, and P. stutzeri but are different enough to represent a novel species. The G+C content of the three strains averaged 61.2 ± 0.8 mol% which is similar to the values reported for P. graminis (61), P. putida (61.6), and P. stutzeri (62.2-65.5). The major cellular fatty acids present in the H2(T) strain were C(18:1) ω7c/C (18:1) ω6c (34.3 %), C(16:1) ω6c/C(16:1) ω7c (27.4 %), C(16:0) (20.6 %), C(12:0) (7.9 %), C(12:0) 3-OH (4.5 %), and C(10:0) 3-OH (3.1 %). The name Pseudomonas kuykendallii sp. nov. is proposed for these bacteria.

Phenotypic and Genomic Characterization of Pseudomonas wuhanensis sp. nov., a Novel Species with Promising Features as a Potential Plant Growth-Promoting and Biocontrol Agent

“…The name Pseudomonas was first proposed by Migula at the end of the 19th century [1]. The genus Pseudomonas includes a diverse microbial population with members that are derived from a wide range of environments, including water, air, soil, plants, and clinical materials [2][3][4][5][6][7][8][9][10]. According to the List of Prokaryotic Names with Standing in Nomenclature (LPSN; https://lpsn.dsmz.de (accessed on 30 December 2023)), Pseudomonas was the second-largest species, and more than 300 species had been validly published with correct names at the time of writing.…”

Why we need a National Living Soil Repository

“…Rapid advances in technology may eventually prove to be useful for the analysis of epigenetics in bacterial populations. Natural soil microbes have been shown to play key roles in engineered industrial processes such as bioenergy (12) and bioremediation (13), as well as contributing to agricultural productivity by modulating disease (14), increasing plant tolerance to abiotic stress (15), and nutrient cycling (16). A living soil repository would preserve genetic diversity as a national asset and serve as a research tool to document baseline changes in soil health, genetic variability, gene function, and population diversity over time.…”