Abstract:A Gram-negative, polar flagella, rod-shaped bacterium LV (T) was isolated from a soil sample subjected to long-term manganese pollution in Hunan Province, China. Cells grow optimally on Luria-Bertani agar medium at 30 °C in the presence of 0-5.0 % (w/v) NaCl and pH 7-8. 16S rRNA gene sequence analysis revealed that strain LV (T) belonged to the genus Pseudomonas, with sequence similarity values of 98.6, 98.2, 98.7, and 97.3 % to Pseudomonas monteilii BCRC 17520 (T) , Pseudomonas putida BCRC 10459 (T) , Pseudom… Show more
“…P. hunanensis , which was identified as a novel species of the genus Pseudomonas in 2014 (Gao et al . 2014), has been reported to be involved in Mn(III)‐L formation and removal from aquatic systems (Wright et al . 2018), as well as in oil degradation (Bakaeva et al .…”
Aims
To isolate, identify and characterize phenolic acid‐degrading bacteria and reduce plant growth inhibition caused by phenolic acids.
Methods and Results
A total of 11 bacterial isolates with high phthalic acid (PA)‐degrading ability were obtained using mineral salt medium (MSM) medium containing PA as sole carbon source. These isolates were identified as Arthrobacter globiformis, Pseudomonas putida and Pseudomonas hunanensis by sequence analyses of the 16S rRNA gene. Among them, five Pseudomonas strains could also effectively degrade ferulic acid (FA), p‐hydroxybenzoic acid (PHBA) and syringic acid (SA) in MSM solution. P. putida strain 7 and P. hunanensis strain 10 showed highly efficient degradation of PA, SA, FA and PHBA, and could reduce their inhibition of lily, watermelon, poplar and strawberry seedling growth in soils respectively. These two strains could promote plant growth in soil with phenolic acids.
Conclusions
In this study, bacterial strains with highly efficient phenolic acid‐degrading abilities could not only effectively reduce the autotoxicity of phenolic acids on plants but also were able to promote plant growth in soil with phenolic acids.
Significance and Impact of the Study
In this study, Pseudomonas can promote plant growth while degrading phenolic acids. Our results provide new choices for the biological removal of autotoxins.
“…P. hunanensis , which was identified as a novel species of the genus Pseudomonas in 2014 (Gao et al . 2014), has been reported to be involved in Mn(III)‐L formation and removal from aquatic systems (Wright et al . 2018), as well as in oil degradation (Bakaeva et al .…”
Aims
To isolate, identify and characterize phenolic acid‐degrading bacteria and reduce plant growth inhibition caused by phenolic acids.
Methods and Results
A total of 11 bacterial isolates with high phthalic acid (PA)‐degrading ability were obtained using mineral salt medium (MSM) medium containing PA as sole carbon source. These isolates were identified as Arthrobacter globiformis, Pseudomonas putida and Pseudomonas hunanensis by sequence analyses of the 16S rRNA gene. Among them, five Pseudomonas strains could also effectively degrade ferulic acid (FA), p‐hydroxybenzoic acid (PHBA) and syringic acid (SA) in MSM solution. P. putida strain 7 and P. hunanensis strain 10 showed highly efficient degradation of PA, SA, FA and PHBA, and could reduce their inhibition of lily, watermelon, poplar and strawberry seedling growth in soils respectively. These two strains could promote plant growth in soil with phenolic acids.
Conclusions
In this study, bacterial strains with highly efficient phenolic acid‐degrading abilities could not only effectively reduce the autotoxicity of phenolic acids on plants but also were able to promote plant growth in soil with phenolic acids.
Significance and Impact of the Study
In this study, Pseudomonas can promote plant growth while degrading phenolic acids. Our results provide new choices for the biological removal of autotoxins.
“…The strain groupings ( P. putida / Pseudomonas monteilii / P. parafulva / P. fulva ; Pseudomonas soli / Pseudomonas mosselii / Pseudomonas entomophila / Pseudomonas plecoglossicida and Pseudomonas donghuensis / Pseudomonas vranovensis / Pseudomonas alkylphenolica ) were maintained in all trees. ‘ Pseudomonas hunanensis ’ NCCB 100446 (proposed as a new species [15], but not yet validated) and Pseudomonas taiwanensis DSM 21245 T branches were supported by low bootstrap values, and their positions varied in the trees.Fig. 1
a Phylogenetic tree based on the almost complete sequence of the 16S rRNA gene of the type strains of the species in the P. putida phylogenetic group.…”
Pseudomonas has the highest number of species out of any genus of Gram-negative bacteria and is phylogenetically divided into several groups. The Pseudomonas putida phylogenetic branch includes at least 13 species of environmental and industrial interest, plant-associated bacteria, insect pathogens, and even some members that have been found in clinical specimens. In the context of the Genomic Encyclopedia of Bacteria and Archaea project, we present the permanent, high-quality draft genomes of the type strains of 3 taxonomically and ecologically closely related species in the Pseudomonas putida phylogenetic branch: Pseudomonas fulva DSM 17717T, Pseudomonas parafulva DSM 17004T and Pseudomonas cremoricolorata DSM 17059T. All three genomes are comparable in size (4.6–4.9 Mb), with 4,119–4,459 protein-coding genes. Average nucleotide identity based on BLAST comparisons and digital genome-to-genome distance calculations are in good agreement with experimental DNA-DNA hybridization results. The genome sequences presented here will be very helpful in elucidating the taxonomy, phylogeny and evolution of the Pseudomonas putida species complex.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-016-0178-2) contains supplementary material, which is available to authorized users.
“…nov. is proposed. It is commonly isolated from soil samples subjected to long-term manganese pollution (Gao et al, 2014). But in this study it was isolated from naturally infected Nile tilapia.…”
Small scale fisheries play important role in providing food and livelihoods for households in Ethiopia. However, prevalence of bacterial fish infection and their antimicrobial resistance are increasing over the world accounting for the major limitations in fish productionas well asits consumption. A cross sectional study was carried out to identify bacterial pathogens isolated from naturally infectedNile tilapia (Oreochromis niloticus) and 20 were common carp (Cyprinus carpio)fish showing clinical signs of disease particularly hemorrhage and skin ulceration and to determine the antibiotic susceptibility of fish associated bacteria in Ethiopia. A total of 42 fish samples from two lakes (Hawassa and Ziway) were aseptically collected and bacteria were isolated from the kidney, liver and intestine. The isolates were identified by their morphological characteristics, biochemical tests and sequencing of 16S rRNA genes. Nine well known fish pathogens were identified from 49 positive samples at the lakes with prevalence rate of Aeromonasveronii(21.43%), Proteusmirabilis (19.05%), Edwardsiella tarda (16.67%), Shigella flexneri(11.9%), Stenotrophomonas maltophilia (11.9%), Aeromonas hydrophila (11.9 %),Pseudomonas putida (9.5%), Aeromonassobria (7.14%) and Pseudomonas hunanensis (7.14%).Of these pathogens, six (6) mostprevalent andknown potential pathogens (A. hydrophila, A. veronii, E. tarda, P. mirabilis, S. flexneri and S. maltophilia)were evaluated for antibiotic susceptibility by using Kirby Bauer disk diffusion assay. All the isolates tested were resistant to at least three (3) of the eight antibiotics evaluated. High levels of resistance were expressed by the majority (87.5%) of the pathogens against penicillin and vancomycin. The results indicate the presence of potential pathogens andmaximum levels of acquired antibiotic resistance in fish bacteria from the study area. Thus, theuse of antibiotic in fish farming in Ethiopia should be discouraged.It isalso advisable to cook fish properly before consumption, in order to kill bacterialpathogens that may be present.
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