Pseudomonas otitidis sp. nov., isolated from patients with otic infections

Clark, Linda; Dajcs, Joseph J.; McLean, Celeste; Bartell, John; Stroman, David W.

doi:10.1099/ijs.0.63753-0

Cited by 77 publications

(47 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Catalase-positive and coagulase-negative S. simiae were previously isolated from heart valve specimens from a patient with infective endocarditis (35). Similarly, P. otitidis was identified from clinical specimens from infected human ears (36). Enterobacter cancerogenus, a facultative anaerobe, has been associated with osteomyelitis (37), urinary tract infections (38), bacteremia, cholangitis, pneumonia (39), and traumatic injuries (40).…”

Section: Discussionmentioning

confidence: 99%

Identification of Polybacterial Communities in Patients with Postoperative, Posttraumatic, and Endogenous Endophthalmitis through 16S rRNA Gene Libraries

et al. 2014

View full text Add to dashboard Cite

‫؍‬ 1), were identified. Interestingly, among them, 10 bacterial species were not previously reported to be associated with endophthalmitis or other ocular infections. Besides, the presence of 4 unidentifiable clones suggests the possibility of novel organisms that might cause eye infections. Therefore, it is recommended that, in addition to the polybacterial nature of POE, PTE, and EE infections, the spectrum of the pathogenic bacterial community identified in this work should be considered while administering antibiotic therapy in suspected endophthalmitis cases.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of Polybacterial Communities in Patients with Postoperative, Posttraumatic, and Endogenous Endophthalmitis through 16S rRNA Gene Libraries

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In our study, identifi cation of some isolates has to be confi rmed due to the controversial information in databases and in some instance equal identity in 16S rDNA sequence was shared with more than one species. As an example, 16S rDNA sequence analysis indicated the presence of P. otitidis, a non-fl uorescent Pseudomonas, which is a highly unexpected result, since the selection criteria included demonstration of fl uorescence on King s B medium (Chang et al, 2008;Clark et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Genetic and functional heterogeneities among fluorescent Pseudomonas isolated from environmental samples

Mehri

Turki

Chérif

et al. 2011

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

“…The P. otitidis strains investigated in this work included 19 strains from the collection previously described by Clark et al (2) and a clinical isolate from South Korea (isolate YMC-Po/06) cultured from the purulent discharge of a patient suffering from chronic suppurative otitis media ( Table 1). Identification of the latter isolate was carried out by 16S rRNA gene sequencing (7) and biochemical profiling (2). Escherichia coli strain MC1061 and Pseudomonas aeruginosa strain PAO1 were used as hosts for recombinant plasmids.…”

Section: Methodsmentioning

confidence: 99%

“…Colonies grown in the presence of the antibiotic were subcultured in antibioticfree MH agar and subjected to reidentification by 16S rRNA gene sequencing and biochemical profiling (2,7).…”

Section: Methodsmentioning

confidence: 99%

Metallo-β-Lactamase Production by Pseudomonas otitidis : a Species-Related Trait

Thaller

Borgianni

Lallo

et al. 2011

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Susceptibility to several ␤-lactams and ␤-lactamase production was investigated in a collection of 20 strains of Pseudomonas otitidis, a new Pseudomonas species that has been recently recognized in association with otic infections in humans. All strains appeared to be susceptible to piperacillin, cefotaxime, ceftazidime, and aztreonam, while resistance or decreased susceptibility to carbapenems was occasionally observed. All strains were found to express metallo-␤-lactamase (MBL) activity and to carry a new subclass B3 MBL gene, named bla POM , that appeared to be highly conserved in this species. P. otitidis, therefore, is the first example of a pathogenic Pseudomonas species endowed with a resident MBL. The POM-1 protein from P. otitidis type strain MCC10330 exhibits the closest similarity (60 to 64%) to the L1 MBL of Stenotrophomonas maltophilia. Expression in Escherichia coli and Pseudomonas aeruginosa revealed that, similar to L1 and other subclass B3 MBLs, POM-1 confers decreased susceptibility or resistance to carbapenems, penicillins, and cephalosporins but not to aztreonam. Expression of the POM MBL in P. otitidis is apparently constitutive and, in most strains, does not confer a carbapenem-resistant phenotype. However, a strong inoculum size effect was observed for carbapenem MICs, and carbapenem-resistant mutants could be readily selected upon exposure to imipenem, suggesting that carbapenem-based regimens should be considered with caution for P. otitidis infections. Pseudomonas otitidis is a newPseudomonas species that has recently been recognized in association with otic infections in humans, including acute otitis externa, acute otitis media, and chronic suppurative otitis media (2). Genotypically and phenotypically, P. otitidis is closely related to Pseudomonas aeruginosa (2), and this similarity likely accounts for the belated identification of this new pathogenic species within the Pseudomonas genus.The susceptibility of P. otitidis was previously investigated with several antimicrobial agents, including aminoglycosides, fluoroquinolones, macrolides, ␤-lactams, tetracycline, chloramphenicol, and polymyxin B, and overall, the behavior of this species appeared to be similar to that of P. aeruginosa (2). However, of ␤-lactams, only piperacillin was tested, while no information is available on the susceptibility of this species to antipseudomonal cephalosporins, aztreonam, and carbapenems.In this work, we investigated the susceptibility of P. otitidis to several ␤-lactams and the production of ␤-lactamase activity by this species. Results revealed that P. otitidis strains constitutively produce a novel subclass B3 metallo-␤-lactamase (MBL), that was named POM (after P. otitidis metallo-␤-lactamase), which is active on carbapenems and other ␤-lactams. MATERIALS AND METHODSBacterial strains. The P. otitidis strains investigated in this work included 19 strains from the collection previously described by Clark et al. (2) and a clinical isolate from South Korea (isolate YMC-Po/06) cultured from the puru...

show abstract

Pseudomonas otitidis sp. nov., isolated from patients with otic infections

Cited by 77 publications

References 11 publications

Identification of Polybacterial Communities in Patients with Postoperative, Posttraumatic, and Endogenous Endophthalmitis through 16S rRNA Gene Libraries

Identification of Polybacterial Communities in Patients with Postoperative, Posttraumatic, and Endogenous Endophthalmitis through 16S rRNA Gene Libraries

Genetic and functional heterogeneities among fluorescent Pseudomonas isolated from environmental samples

Metallo-β-Lactamase Production by Pseudomonas otitidis : a Species-Related Trait

Contact Info

Product

Resources

About