Pseudomonas paucimobilis, a New Species Isolated from Human Clinical Specimens, the Hospital Environment, and Other Sources

Holmes, B.; Owen, Robert J.; Evans, Andrea; Malnick, Henry; Willcox, W. R.

doi:10.1099/00207713-27-2-133

Cited by 175 publications

(130 citation statements)

References 16 publications

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“…Full details of the sources, phenotypic characteristics and clinical significance of the strains of P . paucimobilis have been published previously (Holmes et al, 1977;Hajiroussou et al, 1979;Peel et al, 1979;Slotnick er al., 1979). The bacteria, except P. echinoides, were grown overnight at 30°C in 200 ml nutrient broth (Oxoid no.…”

Section: E T H O D Smentioning

confidence: 96%

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The Similarities Between Pseudomonas paucimobilis and Allied Bacteria Derived from Analysis of Deoxyribonucleic Acids and Electrophoretic Protein Patterns

Owen¹,

Jackman²

1982

Microbiology

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The chromosomal DNA was isolated and purified from 17 strains of Pseudomonas paucimobilis, and from the type or reference strains of Flavobacterium capsulatum, F. devorans, F. multivorum, 'Chromobacterium lividum', Xanthomonas campestris and seven species of Pseudomonas. The DNA base compositions (molx G + C) of P. paucimobilis strains were between 62-2 and 68.6%, and typical strains had a mean value of 65-3 0.4mol %, determined from thermal denaturation temperature. DNA-DNA molecular hybridization with 3H-labelled probe DNA from NCTC 11030 P. paucimobilis (the type strain) indicated that the species comprised a core of 13 closely related strains (74 to 96%), which included F. devorans NCIB 8195 (= ATCC 10829).Four P. paucimobilis strains displayed lower levels of hybridization ( < 38 %). The hybridization results showed that P. paucimobilis was not closely related to allied yellow-pigmented bacteria or to other reference pseudomonads. The electrophoretic protein patterns of representative strains were analysed by computer-assisted techniques and similarity coefficients were calculated. A high degree of congruence was obtained with the DNA hybridization data, and the protein analyses indicated that the four atypical P. paucimobilis strains were heterogeneous and not a single group within the species. The generic affinities of P. paucimobilis, P. capsulatum, 'P.azotocolligans' and P. echinoides are uncertain, but available chemotaxonomic data indicate these species could provide the basis for a new genus.

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Section: E T H O D Smentioning

confidence: 96%

“…The strains previously known as Group IIk, biotype 2 (Tatum et al, 1974), which resembled P. paucimobilis in phenotypic characteristics, were named F. multivorum . The new species differed from P. paucimobilis both in its DNA base composition (Holmes et al, 1977) and in its cellular fatty acid composition (Dees et al, 1979).…”

mentioning

confidence: 99%

The Similarities Between Pseudomonas paucimobilis and Allied Bacteria Derived from Analysis of Deoxyribonucleic Acids and Electrophoretic Protein Patterns

Owen¹,

Jackman²

1982

Microbiology

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“…and Sphingobacterium sp. In addition to this, the results of a phylogenetic analysis of partial nucleotide sequences of the 16S rDNA led to the proposal of a new genus, Sphingomonas Yabuuchi et al 1990" with the type species Sphingomonas paucimobilis (Holmes et al 1977) Yabuuchi et al 1990' (34). In this new genus, three new species, S. adhaesiva, S. parapaucimobilis and S. vanoikuyae, and a new combination, S. capsulata (Leifson 1962AL) Yabuuchi et al 1990' were named and described.…”

mentioning

confidence: 99%

Proposal of Sphingomonadaceae Fam. Nov., Consisting of Sphingomonas Yabuuchi et al. 1990, Erythrobacter Shiba and Shimidu 1982, Erythromicrobium Yurkov et al. 1994, Porphyrobacter Fuerst et al. 1993, Zymomonas Kluyver and van Niel 1936, and Sandaracinobacter Yurkov et al. 1997, with the Type Genus Sphingomonas Yabuuchi et al. 1990

Kosako

Yabuuchi

Naka

et al. 2000

Microbiology and Immunology

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Based on the results of phylogenetic analysis of the 16S rDNA sequences and the presence of N‐2′‐hydroxymyristoyl dihydrosphingosine 1‐glucuronic acid (SGL‐1) and 2‐hydroxymyristic acid (non‐hydroxymyristic acid in Zymomonas) in cellular lipids, a new family, Sphingomonadaceae, for Group 4 of the alpha‐subclass of the class Proteobacteria is herein proposed and a description of the family is given. The family consists of six genera, Sphingomonas, Erythrobacter, Erythromicrobium, Porphyrobacter, Sandaracinobacter and Zymomonas. Thus, all the validly published and currently known genera in Group 4 of the alpha‐subclass of Proteobacteria belong to Sphingomonadaceae fam. nov. Among them, type strains of two species, Porphyrobacter and Erythrobacter, Sandaracinobacter sibiricus and Sphingomonas ursincola, respectively, are facultatively photosynthetic due to bacteriochlorophyll (Bchl)‐a. The type strains of two subspecies of Zymomonas mobilis are facultative anaerobes. The presence of SGL‐1 together with the results of a phylogenetic analysis of the 16S rDNA sequences recommends a new criteria by which to define the new family Sphingomonadaceae. The type genus is Sphingomonas Yabuuchi et al. 1990.

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“…The arginine-negative group also included almost all the brightly yellowpigmented strains that we have seen, all of which were P. paucimobilis (Holmes et al, 1977). We have seen very few true Flavobacterium species and no group Ve bacilli (Weaver et al, 1972).…”

Section: Discussionmentioning

confidence: 77%

The identification of pseudomonads and related bacteria in a clinical laboratory

King

Phillips

1978

Journal of Medical Microbiology

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Pseudomonas paucimobilis, a New Species Isolated from Human Clinical Specimens, the Hospital Environment, and Other Sources

Cited by 175 publications

References 16 publications

The Similarities Between Pseudomonas paucimobilis and Allied Bacteria Derived from Analysis of Deoxyribonucleic Acids and Electrophoretic Protein Patterns

The Similarities Between Pseudomonas paucimobilis and Allied Bacteria Derived from Analysis of Deoxyribonucleic Acids and Electrophoretic Protein Patterns

The identification of pseudomonads and related bacteria in a clinical laboratory

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