Pseudomonas taeanensis sp. nov., isolated from a crude oil-contaminated seashore

Abstract

A novel Gram-negative, aerobic, motile, short rod-shaped bacterium, designated MS-3 T , was isolated from a crude oil-contaminated seashore in Taean, Korea. Strain T grew at 4-30 6C, at pH 6.0-9.5 and with 0-5 % NaCl and was oxidase-and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MS-3 T was most similar toPseudomonas marincola KMM 3042 T (97.9 % 16S rRNA gene sequence similarity), P. Many novel Pseudomonas species have been reported and the classification of the genus Pseudomonas has been reassessed several times on the basis of physiological, molecular and phenotypic features (Sneath et al., 1981), DNA-DNA hybridization (Palleroni, 1984), 16S rRNA gene sequence similarity (Anzai et al., 2000) and chemotaxonomic data (Oyaizu & Komagata, 1983; Vancanneyt et al., 1996). Members of the genus Pseudomonas are ubiquitous and the genus is metabolically possibly the most versatile known so far (Palleroni, 1993;Elkin & Geddes, 2003; Ló pezRomalde et al., 2003;Levitski-Heikkila & Ullian, 2005). A variety of human-made pollutants are degraded by many Pseudomonas strains (Kiyohara et al., 1992; Johnsen et al., 1996; Kahng et al., 2002;Stolz et al., 2007) T was isolated from an oil-contaminated marine coastal area and was found to be capable of degrading gasoline, diesel and kerosene. Further studies were performed to determine its taxonomic position.Water samples that were severely contaminated with crude oil were collected from the Taean coastal area in Korea in 2008. Enrichment cultures were performed in a basal salts medium (Mikesell et al., 1993) containing 0.25-2 % crude oil. Strain MS-3 T was isolated using a standard serial dilution-plating method on marine agar 2216 (MA; Difco) at 25 u C for 5 days. Strain MS-3 T was routinely subcultivated on LB agar (Difco) at 30 u C for 3 days under aerobicThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain T is FJ424813.A transmission electron micrograph of a cell of strain MS-3 T and results from slot-blot DNA-DNA hybridizations and GN2 MicroPlate assays for strain T and its closest relatives are available as supplementary material with the online version of this paper. conditions and stored at 280 u C in LB broth (Difco) supplemented with 15 % (v/v) glycerol.Genomic DNA from strain MS-3 T was extracted and purified by using a genomic DNA extraction kit (Bioneer) and the 16S rRNA gene was amplified using bacterial universal primers (Weisburg et al., 1991). Sequencing of the 16S rRNA gene was carried out as described by Lane (1991). The nearly complete 16S rRNA gene sequence was compared with those available in GenBank using BLAST (http://www. ncbi.nlm.nih.gov/blast/) to determine the approximate phylogenetic affiliation. CLUSTAL W (Thompson et al., 1994) was used to align sequences from closely related taxa. Sequence similarity values were computed using Similarity Matrix version 1.1 (Ribosomal Database Project II; http://rdp.cme. msu.edu/index.jsp) (Cole et al., 2003) and the EzTaxon server (h...

Isolation and Characterization of Oil-Degrading Microorganisms for Bench-Scale Evaluations of Autochthonous Bioaugmentation for Soil Remediation

“…These three genera contained many hydrocarbon degraders that were commonly found in terrestrial environments. Genus Pseudomonas includes many species that are commonly associated with the biodegradation of petroleum hydrocarbons (Lee et al 2010;Salgado-Brito et al 2007;Whyte et al 1997). Genus Nocardia are aerobic and Gram-positive actinomycetes that are widely distributed in aquatic and terrestrial environments.…”

Pseudomonas aestuarii sp. nov., isolated from tidal flat sediment

“…The 16S rRNA gene sequence similarity between strains SA3-5 T and SA3-6 was 100%. On the basis of 16S rRNA gene sequences, SA3-5 T and SA3-6 were most closely related to Pseudomonas taeanensis MS-3 T (98.3 %) [28] and Pseudomonas tumuqii LAMW06 T (98.3%) [29]. The relationship among related species of the genus Pseudomonas and SA3-5 T and SA3-6 is shown in the phylogenetic trees reconstructed by using the neighbour-joining and maximum-likelihood algorithms, in which the novel strains formed a clade with P. tumuqii LAMW06 T (Fig.…”

Draft Genome Sequence of Petroleum Oil-Degrading Marine Bacterium Pseudomonas taeanensis Strain MS-3, Isolated from a Crude Oil-Contaminated Seashore

“…Pseudomonas taeanensis MS-3 T , isolated from crude oil-contaminated seashore in Taean, South Korea, was reported as a novel species of Pseudomonas capable of degrading petroleum oils, such as gasoline, diesel, and kerosene (9). P. taeanensis MS-3 T degraded approximately 80% of gasoline and 100% of kerosene and diesel, all of which were used at 3% concentration during 7 days of incubation under aerobic conditions.…”