Pseudomonas taiwanensis sp. nov., isolated from soil

Wang, Liting; Tai, Chun-Ju; Wu, Yen-Chi; Chen, Ying-Bei; Lee, Fwu-Ling; Wang, San-Lang

doi:10.1099/ijs.0.014779-0

Cited by 58 publications

(32 citation statements)

References 33 publications

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“…The predominant fatty acids of this isolate were summed feature 3 (C 16:1 ω7c and/or iso‐C 15:0 2‐OH) (30.1%), C 16:0 (24.7%) and C 18:1 ω7c (15.0%), which were similar to those of the P. plecoglossicida reference strain BCRC 17517 (29.2%, 23.3% and 19.9%, respectively) described by Wang et al . () (Table ).…”

Section: Resultsmentioning

confidence: 99%

Visceral granulomas in farmed large yellow croaker, Larimichthys crocea (Richardson), caused by a bacterial pathogen, Pseudomonas plecoglossicida

Zhang

Zhou

et al. 2013

Journal of Fish Diseases

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An enzootic disease characterized by granulomas in internal organs occurred in cage-farmed large yellow croaker, Larimichthys crocea (Richardson), in April and November 2010, in Ningbo, Zhejiang Province. One bacterial strain, named XSDHY-P, was isolated from the diseased fish and identified by biochemical characterization, fatty acid methyl ester (FAME) analysis and multilocus sequence analysis (MLSA). According to the results obtained from the biochemical tests, FAME analysis and phylogenetic analysis derived from 16S ribosomal RNA, gyrB, oprF, oprI, oprL and rpoD gene sequencing, the bacterial isolate, XSDHY-P, was identified as Pseudomonas plecoglossicida. Moreover, lethal dose, 50% trials were carried out to demonstrate the virulence of XSDHY-P in large yellow croaker when administered at 2.13 9 105 colony-forming units per fish. Visceral granulomas were found in the experimentally infected fish as well as in the naturally infected fish, indicating that P. plecoglossicida is another bacterial pathogen that causes granulomatosis in L. crocea.

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Section: Resultsmentioning

confidence: 99%

Visceral granulomas in farmed large yellow croaker, Larimichthys crocea (Richardson), caused by a bacterial pathogen, Pseudomonas plecoglossicida

Zhang

Zhou

et al. 2013

Journal of Fish Diseases

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“…nov. CMS T (= BCRC17751 T = DSM 21245 T ) was isolated from soil and characterized using phenotypic and molecular taxonomic methods32. Xanthomonas oryzae pv.…”

Section: Methodsmentioning

confidence: 99%

Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis

Chen

Kuo

Hsieh

et al. 2016

Sci Rep

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Rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive rice diseases worldwide. Therefore, in addition to breeding disease-resistant rice cultivars, it is desirable to develop effective biocontrol agents against Xoo. Here, we report that a soil bacterium Pseudomonas taiwanensis displayed strong antagonistic activity against Xoo. Using matrix-assisted laser desorption/ionization imaging mass spectrometry, we identified an iron chelator, pyoverdine, secreted by P. taiwanensis that could inhibit the growth of Xoo. Through Tn5 mutagenesis of P. taiwanensis, we showed that mutations in genes that encode components of the type VI secretion system (T6SS) as well as biosynthesis and maturation of pyoverdine resulted in reduced toxicity against Xoo. Our results indicated that T6SS is involved in the secretion of endogenous pyoverdine. Mutations in T6SS component genes affected the secretion of mature pyoverdine from the periplasmic space into the extracellular medium after pyoverdine precursor is transferred to the periplasm by the inner membrane transporter PvdE. In addition, we also showed that other export systems, i.e., the PvdRT-OpmQ and MexAB-OprM efflux systems (for which there have been previous suggestions of involvement) and the type II secretion system (T2SS), are not involved in pyoverdine secretion.

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“…Pseudomonas taiwanensis is a novel Gram-negative bacterium isolated from soils that can grow on medium with shrimp shell powder as the sole carbon and nitrogen source [20]. Interestingly, P. taiwanensis displays high levels of extracellular chitinasae, chitosanase, and nattokinase activities under shrimp shell medium [21], [22].…”

Section: Introductionmentioning

confidence: 99%

Characterization of an Insecticidal Toxin and Pathogenicity of Pseudomonas taiwanensis against Insects

et al. 2014

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Pseudomonas taiwanensis is a broad-host-range entomopathogenic bacterium that exhibits insecticidal activity toward agricultural pests Plutella xylostella, Spodoptera exigua, Spodoptera litura, Trichoplusia ni and Drosophila melanogaster. Oral infection with different concentrations (OD = 0.5 to 2) of wild-type P. taiwanensis resulted in insect mortality rates that were not significantly different (92.7%, 96.4% and 94.5%). The TccC protein, a component of the toxin complex (Tc), plays an essential role in the insecticidal activity of P. taiwanensis. The ΔtccC mutant strain of P. taiwanensis, which has a knockout mutation in the tccC gene, only induced 42.2% mortality in P. xylostella, even at a high bacterial dose (OD = 2.0). TccC protein was cleaved into two fragments, an N-terminal fragment containing an Rhs-like domain and a C-terminal fragment containing a Glt symporter domain and a TraT domain, which might contribute to antioxidative stress activity and defense against macrophagosis, respectively. Interestingly, the primary structure of the C-terminal region of TccC in P. taiwanensis is unique among pathogens. Membrane localization of the C-terminal fragment of TccC was proven by flow cytometry. Sonicated pellets of P. taiwanensis ΔtccC strain had lower toxicity against the Sf9 insect cell line and P. xylostella larvae than the wild type. We also found that infection of Sf9 and LD652Y-5d cell lines with P. taiwanensis induced apoptotic cell death. Further, natural oral infection by P. taiwanensis triggered expression of host programmed cell death-related genes JNK-2 and caspase-3.

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Pseudomonas taiwanensis sp. nov., isolated from soil

Cited by 58 publications

References 33 publications

Visceral granulomas in farmed large yellow croaker, Larimichthys crocea (Richardson), caused by a bacterial pathogen, Pseudomonas plecoglossicida

Visceral granulomas in farmed large yellow croaker, Larimichthys crocea (Richardson), caused by a bacterial pathogen, Pseudomonas plecoglossicida

Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis

Characterization of an Insecticidal Toxin and Pathogenicity of Pseudomonas taiwanensis against Insects

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