Pseudomonas: The Versatile and Adaptive Metabolic Network

Singh, Partap; Saini, Harvinder Singh; Kahlon, R. S.

doi:10.1007/978-3-319-31198-2_3

Cited by 12 publications

(14 citation statements)

References 111 publications

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“…These data are consistent with P. aeruginosa carbon source preferences (Palmer et al, 2007 ). The low glucose consumption was further supported by the RNAseq data that showed no induction of the main genes involved in the glucose catabolism (Supplementary Table 3 ) (reviewed in Singh et al, 2016 ). Interestingly, despite stable expression of glucose porin oprB (PA3186), both glucose dehydrogenase gcd (PA2290) and gluconate permase gnuT (PA2322) showed induction in BWE compared to LB control conditions (Supplementary Table 3 ).…”

Section: Resultsmentioning

confidence: 69%

Transcriptome Analysis of Pseudomonas aeruginosa Cultured in Human Burn Wound Exudates

Gonzalez

Ducret

Leoni

et al. 2018

Front. Cell. Infect. Microbiol.

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Pseudomonas aeruginosa is a severe opportunistic pathogen and is one of the major causes of hard to treat burn wound infections. Herein we have used an RNA-seq transcriptomic approach to study the behavior of P. aeruginosa PAO1 growing directly on human burn wound exudate. A chemical analysis of compounds used by this bacterium, coupled with kinetics expression of central genes has allowed us to obtain a global view of P. aeruginosa physiological and metabolic changes occurring while growing on human burn wound exudate. In addition to the numerous virulence factors and their secretion systems, we have found that all iron acquisition mechanisms were overexpressed. Deletion and complementation with pyoverdine demonstrated that iron availability was a major limiting factor in burn wound exudate. The quorum sensing systems, known to be important for the virulence of P. aeruginosa, although moderately induced, were activated even at low cell density. Analysis of bacterial metabolism emphasized importance of lactate, lipid and collagen degradation pathways. Overall, this work allowed to designate, for the first time, a global view of P. aeruginosa characteristics while growing in human burn wound exudate and highlight the possible therapeutic approaches to combat P. aeruginosa burn wound infections.

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Section: Resultsmentioning

confidence: 69%

Transcriptome Analysis of Pseudomonas aeruginosa Cultured in Human Burn Wound Exudates

Gonzalez

Ducret

Leoni

et al. 2018

Front. Cell. Infect. Microbiol.

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“…Potential successful bacterial competitors might therefore be expected to encode an array of mechanisms allowing them to emerge and dominate particular bacterial populations. Bacteria belonging to the genus Pseudomonas have a highly variable genome size from 3.7 to 7.1 Mb, containing more than 6390 predicted genes ( Singh et al, 2016 ). This is consistent with their capacity to produce an extensive repertoire of organic compounds including simple carbohydrates as well as more complex biomolecules such as biosurfactants, insecticides, phytotoxic compounds, antibiotics and siderophores, which enable them to compete in highly diverse ecological niches.…”

Section: Discussionmentioning

confidence: 99%

Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity

et al. 2020

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The bacterium Pseudomonas entomophila has been recognized as an exceptional species within the Pseudomonas genus, capable of naturally infecting and killing insects from at least three different orders. P. entomophila ingestion leads to irreversible gut damage resulting from a global blockage of translation, which impairs both immune and tissue repair systems in the insect intestine. In this study we isolated a P. entomophila bacterial strain from soil samples which displayed a strong activity against Xanthomonas citri subsp, citri (Xcc), the etiological agent of citrus canker disease. The antagonism potential of isolated bacteria against Xcc and its ability to reduce citrus canker severity was assessed both ex planta and in planta. Our findings show that pathogenicity assays in Citrus x limonia by pressure infiltration and spray with a mixture of P. entomophila and Xcc leaded to a significant reduction in the number of canker lesions in high susceptible citrus leaves, at 21 days post-infection. To the best of our knowledge this is the first report of antibacterial activity of P. entomophila against a phytopathogenic bacterium. Collective action of P. entomophila factors such as diketopiperazine production and the type 6 secretion system (T6SS) may be involved in this type of biological control of citrus canker. The results suggest that the P. entomophila strain could be a promising biocontrol agent acting directly against Xcc.

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“…Pseudomonas spp. are reported to be able to degrade aromatic compounds in two steps, beginning with a ring modification to yield a catechol (upper pathway) followed by a ring fission by either ortho-or meta-cleavage (lower pathway) [4].…”

Section: Degradation Of Aromatic Compoundsmentioning

confidence: 99%

“…They grow rapidly under simple nutritional conditions and some species are able to use more than 100 different sources of carbon and energy. Members of the genus Pseudomonas may catabolize aromatic and aliphatic hydrocarbons (phenols, toluene, n-alkanes, cyclohexane) by using specific pathways [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomic Analysis of the Biotechnological Potential of the Novel Species Pseudomonas wadenswilerensis CCOS 864T and Pseudomonas reidholzensis CCOS 865T

et al. 2019

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In recent years, the use of whole-cell biocatalysts and biocatalytic enzymes in biotechnological applications originating from the genus Pseudomonas has greatly increased. In 2014, two new species within the Pseudomonas putida group were isolated from Swiss forest soil. In this study, the high quality draft genome sequences of Pseudomonas wadenswilerensis CCOS 864T and Pseudomonas reidholzensis CCOS 865T were used in a comparative genomics approach to identify genomic features that either differed between these two new species or to selected members of the P. putida group. The genomes of P. wadenswilerensis CCOS 864T and P. reidholzensis CCOS 865T were found to share genomic features for the degradation of aromatic compounds or the synthesis of secondary metabolites. In particular, genes encoding for biocatalytic relevant enzymes belonging to the class of oxidoreductases, proteases and isomerases were found, that could yield potential applications in biotechnology. Ecologically relevant features revealed that both species are probably playing an important role in the degradation of soil organic material, the accumulation of phosphate and biocontrol against plant pathogens.

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Pseudomonas: The Versatile and Adaptive Metabolic Network

Cited by 12 publications

References 111 publications

Transcriptome Analysis of Pseudomonas aeruginosa Cultured in Human Burn Wound Exudates

Transcriptome Analysis of Pseudomonas aeruginosa Cultured in Human Burn Wound Exudates

Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity

Comparative Genomic Analysis of the Biotechnological Potential of the Novel Species Pseudomonas wadenswilerensis CCOS 864T and Pseudomonas reidholzensis CCOS 865T

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