Pseudonocardia mongoliensis sp. nov. and Pseudonocardia khuvsgulensis sp. nov., isolated from soil

During the course of our research on new actinobacterial sources, a novel actinomycete strain YIM 63101(T) was isolated from the surface-sterilized roots of Artemisia annua L. collected from Xishuangbanna, Yunnan province, south-west China and characterized by using a polyphasic approach. The strain formed well-differentiated aerial and substrate mycelia. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 63101(T) belongs to the genus Pseudonocardia, with highest similarity to "Pseudonocardia artemisiae YIM 63587(T)" (99.4%). Sequence similarities between strain YIM 63101(T) and the other Pseudonocardia species ranged from 97.0 (Pseudonocardia saturnea IMSNU 20052(T)) to 94.0% (Pseudonocardia compacta IMSNU 20111(T)). The chemotaxonomic characteristics, such as cell wall diaminopimelic acid, whole-cell sugars, fatty acid components and the major menaquinones suggested that the organism belonged to the genus Pseudonocardia. The G + C content of the genomic DNA was 69.4 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including low DNA-DNA hybridization results, it is proposed that strain YIM 63101(T) represents a novel species of the genus Pseudonocardia, named Pseudonocardia bannaensis sp. nov. The type strain is YIM 63101(T) (= CCTCC AA 208077 (T) = DSM 45300(T)).

Section: Introductionmentioning

confidence: 93%

Pseudonocardia bannaensis sp. nov., a novel actinomycete isolated from the surface-sterilized roots of Artemisia annua L.

Zhao

Zhu

et al. 2011

“…net/pseudonocardia.html). Bacterial species of this genus have been isolated from various environments, such as soil (Henssen et al 1983;Lee et al 2002;Park et al 2008;Qin et al 2008;Ara et al 2011), plant roots (Duangmal et al 2009;Kaewkla and Franco 2011;Zhao et al 2011), plant stem (Qin et al 2010), coastal sediment (Liu et al 2006), deep-sea sediment (Tian et al 2013), industrial sludge (Mahendra and AlvarezCohen 2005) and a gold mine cave (Lee et al 2001). Mangrove ecosystems, as highly productive wetlands distributed along estuaries, provide refuge, growing, breeding and feeding zones for many marine organisms (Holguin et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Pseudonocardia nematodicida sp. nov., isolated from mangrove sediment in Hainan, China

Liu

Shen

Yuan

et al. 2015

Two aerobic, Gram-stain positive actinobacterial strains with nematicidal activity, designated HA11164(T) and HA12591, were isolated from mangrove sediments in Hainan, China. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strains HA11164(T) and HA12591 belong to the genus Pseudonocardia and are closely related to Pseudonocardia carboxydivorans (with the similarities of 98.30 and 98.24 %, respectively), Pseudonocardia alni (98.23 and 98.16 %, respectively) and Pseudonocardia antimicrobica (98.10 and 98.03 %, respectively). The major polar lipids of the strain HA11164(T), as a representative strain of the two strains, were found to consist of phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, five unidentified glycolipids and four unidentified polar lipids. The predominant menaquinone of strain HA11164(T) was identified as MK-8 (H4), and the major fatty acids were identified as iso-C16:0, C17:1 ω10, C16:0 and C16:1 ω9. The G+C content of strain HA11164(T) was determined to be 74.9 mol%. The DNA-DNA relatedness values between strains HA11164(T) and P. alni, Pseudonocardia tropica, Pseudonocardia antarctica, P. carboxydivorans and Pseudonocardia parietis were 58.3, 56.2, 50.0, 57.1 and 46.0 %, respectively. Based on the results of this polyphasic study, strains HA11164(T) and HA12591 are considered to represent a novel species of the genus Pseudonocardia, for which the name Pseudonocardia nematodicida sp. nov. is proposed. The type strain is HA11164(T) (=CGMCC 4.7118(T) = DSM 45940(T)).

“…So far, the genus Pseudonocardia encompasses 44 species with validly published names (http://www.bacterio.cict.fr/; Ara et al 2011;Franco 2010, 2011;Li et al 2010;Qin et al 2010Qin et al , 2011Sakiyama et al 2010;Zhao et al 2011a). Members of the genus Pseudonocardia have the characteristics as follow: have the type IV cell wall, aerial and vegetative mycelium, display spore chains which are produced by acropetal budding or fragmentation, have MK-8 (H 4 ) or MK-9 as their major menaquinone, iso-branched hexadecanoic acid as the predominant fatty acid.…”

Section: Introductionmentioning

confidence: 99%

Pseudonocardia yuanmoensis sp. nov., a novel actinobacterium isolated from soil in Yunnan, south-west China

Nie

Hong

Wei

et al. 2011

A novel Gram-stain positive, aerobic, non-motile, spore-forming actinobacterium, designated YIM 75926(T), was isolated from a soil sample collected at soil forest in Yuanmo county of Yunnan province, south-west China. Its taxonomic position was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain YIM 75926(T) belongs to the genus Pseudonocardia and was closely related to Pseudonocardia halophobica DSM 43089(T) (98.1% similarity). Strain YIM 75926(T) had MK-8 (H(4)) as the predominant menaquinone. The whole organism hydrolysates mainly consisted of meso-diaminopimelic acid, mannose, glucose, galactose and arabinose. The major cellular fatty acids were iso-C(16:0) (37.16%) and C(16:0) (12.43%). The DNA G+C content of strain YIM 75926(T) was 70.6 mol%. The resultant phylogenetic trees further showed that strain YIM 75926(T) belong to Pseudonocardia and had a distinct subclade within the evolutionary radiation of the genus Pseudonocardia. On the basis of its comparative analysis of phenotypic and genotypic characteristics, it is proposed that strain YIM 75926(T) represent a novel species of the genus Pseudonocardia, named Pseudonocardia yuanmoensis sp. nov. The type strain is YIM 75926(T) (=CCTCC AA 2011017(T) = JCM 18055(T)).