Pseudouridine-deficient transfer RNAs from Escherichia coli B and their use as substrates for pseudouridine synthetase.

Effects of 5-fluorouracil on base modification in Escherichia coli tRNA

“…Kaiser, 1978, unpublished data) and others (Baliga et al, 1969) have shown E. coli FUra-tRNAs capable of accepting methyl groups in vitro with heterologous and homologous methylases. It has also been shown that mature tRNAs that are methyl-and pseudouridine deficient can serve as substrates for ribosylthymidine synthetase and pseudouridine synthetase in vitro (see Shugart, 1978, and references therein;Cortese et al, 1974;Kwong et al, 1977). It is not yet known whether dihydrouridine synthetase can function on maturesized tRNAs or whether it requires the precursor form.…”