Pseudozyma sp. SPJ: an economic and eco-friendly approach for degumming of flax fibers

Sharma, Sampriya; Mandhan, Rishi Pal; Sharma, Jitender

doi:10.1007/s11274-011-0743-1

Cited by 22 publications

(9 citation statements)

References 26 publications

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“…After the lignin has been removed by alkali, hemicellulose depolymerization occurs; the reducing end-group of the hemicellulose chain is oxidized (Keller et al 2001;Sinha and Rout 2008;Sawpan et al 2011;Sharma et al 2011). Cellulose is easily hydrolyzed by acid and easily oxidized.…”

Section: Peer-reviewed Articlementioning

confidence: 99%

“…Wetting allows the hemp fiber to swell and become loose, which improves the penetration of scouring agents. Under alkaline conditions, lignin reacts with alkali, and the ether bonds present in the compounds of α-phenyl ether, α-alkyl ether, β-aryl ether, and aryl ether linked with lignin would be attacked by nucleophile of OH -and broken, In addition, the reaction degrades and dissolves large molecules such as lignin.After the lignin has been removed by alkali, hemicellulose depolymerization occurs; the reducing end-group of the hemicellulose chain is oxidized (Keller et al 2001;Sinha and Rout 2008;Sawpan et al 2011;Sharma et al 2011). Cellulose is easily hydrolyzed by acid and easily oxidized.…”

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An Efficient Method of Bio-Chemical Combined Treatment for Obtaining High-Quality Hemp Fiber

Fang

Chen

et al. 2017

BioResources

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This bio-chemical study focuses on obtaining high-quality hemp fiber. The effects of the structures and properties of hemp fibers in different treatment periods were studied. Moreover, the changes of the surface morphology, chemical composition, and breaking tenacity of hemp fibers were researched by scanning-electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), fluorescence microscopy, and fiber tensile testing. The results showed that by virtue of the enzyme scouring process, alkali refining process, and bleaching process, the pectin, lignin, and hemicellulose and other impurities were removed. Through the single factor experiment, the optimal process conditions for the bio-chemical combination of the degumming process were obtained. These conditions included 10 g of dried hemp fibers, 15% (v/v) pectinase solution, a temperature of 50 °C, a duration of 120 min, pH 8.0 (phosphate buffer), a liquor ratio (w/v) of 1:10, and 0.0625 mol/L NaOH. In these conditions, the residual gum content and breaking tenacity were 4.8% and 49.8 cN/tex, respectively, indicating that the treated hemp fibers met the requirements of the spinning process.Keywords: Hemp; Degumming; Residual gum Contact information: School of Environmental Engineering, Wuhan Textile University, Wuhan, Hubei Province, 430073, China; *Corresponding author: honggao_chen@hotmail.com INTRODUCTIONHemp fibers have relatively low fineness and no scratchiness, which promotes their high hygroscopicity, gas permeability, and other excellent properties. In addition, these fibers are resistant to bacteria, mold, and ultraviolet rays, and they can be used in healthcare applications (Mohanty et al. 2002;Keller 2003;Khan et al. 2014). With the low content of tetrahydrocannabinol (THC < 0.3%) that has been achieved by breeding of hemp varieties (Lachenmeier et al. 2004), hemp has gradually come to the forefront of textile applications. The hemp fiber can be blended and spun with cotton, silk, wool, and other chemical fibers, and it can also be spun into mono-fibers. However, the phloem of hemp contains hemicellulose, lignin, pectin, wax, and other chemical impurities that must be removed from the fiber surface in a key process called degumming (George et al. 2015). Degumming has significant effects on spinning quality, fabric style, and comfort. There is a direct relationship between the degumming quality and fiber properties (Sreenath et al. 1996;Nair et al. 2015).Compared with ramie fiber, hemp fiber has higher hemicellulose and lignin contents, which directly affects its spinning performance. Commonly used degumming methods include chemical degumming (Di Candilo et al. 2000;Wang et al. 2003;Thomsen et al. 2006), physical degumming (Vignon et al. 1995Dupeyre and Vignon 1998;Guo and Zhao 2010), and biological degumming (Nykter et al. 2008). PEER-REVIEWED ARTICLEbioresources.com Fang et al. (2017). "Hemp fiber degumming," BioResources 12(1), 1566-1578. 1567Because the water resistance of hemp is very strong, the fiber is difficult to ferme...

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Section: Peer-reviewed Articlementioning

confidence: 99%

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confidence: 99%

An Efficient Method of Bio-Chemical Combined Treatment for Obtaining High-Quality Hemp Fiber

Fang

Chen

et al. 2017

BioResources

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“…S27, Paenibacillus campinasensis, and Geobacillus thermoglucosidasius (Ko et al, 2011;Sharma et al, 2011;Valladares Juarez et al, 2009;Yuan et al, 2012). These bacterial strains are typical pectin-degrading bacteria that degum bast fibers to different degrees; however, these strains require long degumming cycles and produce low-quality fiber with high residual gum rate, which cannot satisfy production requirements of the bast fiber processing industry.…”

Section: Introductionmentioning

confidence: 99%

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Cheng

Wang

Feng

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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ABSTRACT:Biological degumming is an eco-friendly, efficient, high-quality and low-cost method that has become the leading bast fiber degumming technology. However, bacterial strains with short degumming cycles, high gum removal rates and small fiber damage are few. To screen high quality microbial resources with bast-fiber biological degumming function, soil samples were collected from a continuously cultivated banana plantation and then used to be enriched by ramie and kenaf materials in turn. A selective pectin-degrading medium was used to screen for excellent bacteria. A dominant bacterial strain was identified by phenotypic and genotypic characteristics, and its biological degumming effects on ramie and kenaf were verified by a comprehensive evaluation system. Results showed that seven bacterial strains secreting pectinase were obtained and the largest hydrolysis circle with a diameter ratio H/C of 2.4 was produced by the bacterial strain hn1-1, which was preliminarily identified as the Bacillus cereus by colony morphological characteristics and 16S rDNA sequence (GenBank accession number: KX013542) cluster analysis. The fiber production of ramie and kenaf degummed by B. cereus hn1-1 for 10 h were 72 % and 76 %, the residual gum rates were 4 % and 5 %, respectively. These values satisfied the textile industry requirement of < 6 % residual gum rate. Therefore, an effective biological degumming bacterium, B. cereus, was identified using a pectin-hydrolysis selective medium through a simple, economical, and time-saving method. Furthermore, the biological degumming technology by B. cereus for ramie and kenaf had a short cycle, ideal removal gum rate, and high-quality and productive fiber output.

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“…The pectinase produced by Pseudozyma sp. SPJ has been produced using cost effective substrate [14,15] and also have been successfully studied for degumming of flax fibers [16].…”

Section: Introductionmentioning

confidence: 99%

Bioprocessing of Crop Residues using Fibrolytic Enzymes and Flavobacterium bolustinum for Enriching Animal Feed

Malik¹,

Sharma²,

Sharma³

et al. 2015

Int. J. Biotech. Well. Indus.

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Flavobacterium bolustinum and its extracellular cellulase were tested for animal feed pretreatment. The fibrolytic enzymes, cellulase and pectinase were applied to various crop residues such as wheat straw, rice straw, corn seeds and sorghum for enriching animal feed. Different parameters like temperature, incubation time and enzyme dose had been optimized for maximum reducing sugar and protein release. The highest amount of reducing sugar obtained was 29.83 mg g-1 dry substrate and soluble protein was 27.34 mg g-1 dry substrate on single cellulase enzyme treatment at 50°C for 6 h. An increase in amount of released reducing sugar (39.5 mg g-1 dry substrate) and protein (33.88 mg g-1 dry substrate) was observed when enzyme cocktail (cellulose and pectinase) was used. Solid state fermentation using F. bolustinum had also been performed for all crop residues. It released higher amount of reducing sugar (41.36 mg g-1) and protein (47.21 mg g-1) as compared to enzymatic treatment. Different substrates resulted in appreciable weight loss by enzymatic treatment (15-35%) as well as fermentation using F. bolustinum (40%). Liquefaction of lignocellulosic rich crop residues, for better utilization of feed has never been reported earlier.

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Pseudozyma sp. SPJ: an economic and eco-friendly approach for degumming of flax fibers

Cited by 22 publications

References 26 publications

An Efficient Method of Bio-Chemical Combined Treatment for Obtaining High-Quality Hemp Fiber

An Efficient Method of Bio-Chemical Combined Treatment for Obtaining High-Quality Hemp Fiber

Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Bioprocessing of Crop Residues using Fibrolytic Enzymes and Flavobacterium bolustinum for Enriching Animal Feed

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