PSIV-B-25 Late-Breaking: Maternal supply of polyunsaturated fatty acids and methionine during late- gestation alters amino acid transporters and global DNA methylation in the lamb small intestine

Abstract

The intestine plays a crucial role in nutrient digestion and absorption, and its function is critical for animal growth and health. However, the effect of maternal nutrition on offspring’s intestine is not well documented. The objective of this study was to evaluate the effects of maternal supply of polyunsaturated fatty acids, methionine or both during late-gestation on offspring’s intestine amino acids transporters and global DNA methylation. Twenty ewes were used in a randomized block design and treatments were arranged in a 2×2 factorial design. Treatments were: 1) control diet (CTR) without polyunsaturated fatty acids (PUFA) or methionine (MET) supplementation; or supplemented with 2) Ca salts of PUFA (1% DMI; Strata G113®, Virtus Nutrition); rumen-protected MET (0.1% DMI; Smartamine®, Adisseo); and 4) PUFA and MET (PUFA+MET). Treatments were fed from day 100 to 145 of gestation. On day 145, ewes were euthanized and fetuses’ intestines were sampled. Protein quantification of amino acid transporters was measured using Simple Western System and global DNA methylation was determined using a commercial kit. No interactions between PUFA×MET were observed for the variables analyzed. PUFA increased (P ≤ 0.03) global DNA methylation and protein expression of SLC7A5, SLC38A2, and SLC38A10 compared with CTR. Furthermore, PUFA tended to increase (P = 0.07) SLC38A1 protein expression compared with CTR, whereas no difference (P > 0.05) was observed for SLC6A9. Compared with CTR, MET increased (P ≤ 0.05) global DNA methylation and SLC38A10 protein expression, and tented to increase (P ≤ 0.10) SLC6A19 and SLC38A2. MET did not affect SLC7A5 and SLC38A1 compared with CTR. Maternal supply of PUFA and MET led to greater protein expression of amino acid transporters and global DNA methylation on offspring’ intestine; however, the effects of these nutrients were not additive. Further research is necessary to better understand the role of DNA methylation on intestine’s amino acid transporters.

Maternal Supply of Ruminally-Protected Lysine and Methionine During Close-Up Period Enhances Immunity and Growth Rate of Neonatal Calves

“…Maternal nutrition can also influence the development of the intestinal tract in ruminants, which can affect the digestibility of nutrients and subsequent performance ( 32 , 65 , 66 ). RPM supply to late-gestation ewes increased both the protein expression of intestinal AA transporters and global methylation ( 32 ).…”

“…Maternal nutrition can also influence the development of the intestinal tract in ruminants, which can affect the digestibility of nutrients and subsequent performance ( 32 , 65 , 66 ). RPM supply to late-gestation ewes increased both the protein expression of intestinal AA transporters and global methylation ( 32 ). Additionally, work on non-ruminants ( 35 ) showed that Met inclusion to late-gestation swine feeds altered the intestinal microbiota and gut morphology of their offspring.…”

“…Research on fetal programming has been conducted on dairy cattle (30), sheep (31,32), and non-ruminants (33)(34)(35), with a particular focus on nutrient manipulation during late pregnancy and its impact on the fetus and postnatal development of the offspring. Therefore, it was hypothesized that maternal supplementation of late gestation dairy cows with ruminally protected Lys (RPL), RPM, or the two in combination would, in addition to benefitting the cows themselves, increase the supply of metabolizable Met and Lys and other essential AAs that reach the calf through uteroplacental transfer, thereby aiding fetal development, contributing to increased bovine colostrum IgG, maximizing passive immunity transfer from cow to calf via colostrum, and enhancing the health status of calves.…”

Programação fetal e as consequências no desenvolvimento da progênie – uma revisão

“…Em estudo semelhante, Cruz et al (2019) não observaram influência da suplementação com proteína para vacas de corte no meio até o final da gestação sobre a digestibilidade dos nutrientes na prole. Resultados opostos foram obtidos por Relling et al (2019), os quais observaram que a suplementação de metionina protegida no rúmen para ovelhas durante a gestação aumentou expressão de proteínas de transportadores de aminoácidos intestinais e metilação global do DNA, ou seja, pode contribuir para a maior expressão de genes favoráveis à digestão e aproveitamento dos nutrientes da dieta.…”