PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae

Li, Aining; Zhang, Meng; Wang, Yonglin; Li, Delong; Liu, Xiaoyun; Tao, Kai; Ye, Wenwu; Wang, Yuanchao

doi:10.1016/j.fgb.2014.01.003

Cited by 37 publications

(28 citation statements)

References 53 publications

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“…Transient elevation of gene expression has also been documented during P. infestans sporangiogenesis from microarrays ). In P. infestans and P. sojae, sporangial development is inhibited by silencing the expression of genes encoding the G-protein b-subunit Pigpb1, the MAPK PsMPK1 and the cell cycle phosphatase PiCdc14 (Ah-Fong and Judelson, 2011;Latijnhouwers and Govers, 2003;Li et al, 2014). Phytophthora cinnamomi has homologues to each of these three proteins (Table 1).…”

Section: Sporangiogenesis: Sporangium Developmentmentioning

confidence: 99%

“…In multiplex assays, differentially tagged primer pairs have been used to simultaneously test for the presence of P. cinnamomi and other pathogen species in the same soil, water or plant samples (Kostov et al, 2016). Specific recognition of DNA sequences has also been used to detect P. cinnamomi in planta through the application of fluorescence in situ hybridization (Li et al, 2014).…”

Section: Detection and Identificationmentioning

confidence: 99%

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Phytophthora cinnamomi

Hardham

Blackman

2017

Molecular Plant Pathology

189

184

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A root pathogen which causes rotting of fine and fibrous roots, but which can also cause stem cankers. Root damage may inhibit water movement from roots to shoots, leading to dieback of young shoots. USEFUL WEBSITES: http://fungidb.org/fungidb/; http://genome.jgi.doe.gov/Phyci1/Phyci1.home.html; http://www.ncbi.nlm.nih.gov/assembly/GCA_001314365.1; http://www.ncbi.nlm.nih.gov/assembly/GCA_001314505.1.

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Section: Sporangiogenesis: Sporangium Developmentmentioning

confidence: 99%

Section: Detection and Identificationmentioning

confidence: 99%

Phytophthora cinnamomi

Hardham

Blackman

2017

Molecular Plant Pathology

189

184

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“…By phosphorylation on MAPK Fmk1, Fusarium oxysporum Fbp1 regulates virulence, cell wall integrity, and invasive growth via the Fmk1 signal pathway (Miguel-Rojas and Hera, 2016 ). MAPKs related to the yeast Slt2, such as SLT2-type MAPK protein PsMPK1, and PsMPK7 from Phytophthora sojae are also important for hyphal growth, cell wall integrity, stress tolerance, ROS detoxification, and pathogenicity (Li et al, 2014 ; Gao et al, 2015 ). Mutation of three MAPK genes FoSlt2, FoMkk2 , and FoBck1 respectively led to attenuated virulence and slower growth rate in F. oxysporum (Ding et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Colletotrichum higginsianum Mitogen-Activated Protein Kinase ChMK1: Role in Growth, Cell Wall Integrity, Colony Melanization, and Pathogenicity

Wei

Xiong²,

Zhu

et al. 2016

Front. Microbiol.

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Colletotrichum higginsianum is an economically important pathogen that causes anthracnose disease in a wide range of cruciferous crops. To facilitate the efficient control of anthracnose disease, it will be important to understand the mechanism by which the cruciferous crops and C. higginsianum interact. A key step in understanding this interaction is characterizing the mitogen-activated protein kinases (MAPK) signaling pathway of C. higginsianum. MAPK plays important roles in diverse physiological processes of multiple pathogens. In this study, a Fus3/Kss1-related MAPK gene, ChMK1, from C. higginsianum was analyzed. The results showed that the Fus3/Kss1-related MAPK ChMK1 plays a significant role in cell wall integrity. Targeted deletion of ChMK1 resulted in a hypersensitivity to cell wall inhibitors, reduced conidiation and albinistic colonies. Further, the deletion mutant was also unable to form melanized appressorium, a specialized infection structure that is necessary for successful infection. Therefore, the deletion mutant loses pathogenicity on A. thaliana leaves, demonstrating that ChMK1 plays an essential role in the early infection step. In addition, the ChMK1 deletion mutant showed an attenuated growth rate that is different from that of its homolog in Colletotrichum lagenarium, indicating the diverse roles that Fus3/Kss1-related MAPKs plays in phytopathogenic fungi. Furthermore, the expression level of three melanin synthesis associated genes were clearly decreased in the albinistic ChMK1 mutant compared to that of the wild type strain, suggesting that ChMK1 is also required for colony melanization in C. higginsianum.

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“…Mycelia were then washed with autoclaved, distilled water, then extracted by filtration. Asexual zoospores were obtained following established methods (36). One minute of vigorous shaking of P. sojae cultures was used to induce the zoospores to encyst.…”

Section: Preparation Of P Sojae Mycelia Cysts and Oosporesmentioning

confidence: 99%

“…The 4-day-old etiolated soybean seedlings (cv. Ribenqing, grown in the dark at 25℃ and 80% humidity) were used for the in planta pathogenicity assays (36). The seedlings were spot inoculated at 2 cm below the beginning of the root zone with 10 ul zoospores suspension (1×10 4 zoospores/ml), and incubated at 25℃ for 2 days in the dark.…”

Section: Phenotypic Analysis Of the Site-directed Mutantsmentioning

confidence: 99%

The consensus N_glyco-X-S/T motif and a previously unknown N_glyco-N -linked glycosylation are necessary for growth and pathogenicity ofPhytophthora

Zhang

Chen

Zhang

et al. 2020

Preprint

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Asparagine (Asn, N) -linked glycosylation within the glycosylation motif (Nglyco-X-S/T; X P) is a ubiquitously distributed post-translational modification that participates in diverse eukaryotic cellular processes. However, little is known about the characteristic features and roles of N-glycosylation in oomycetes. In this work, it found that 2.5 μg/ml tunicamycin (N-glycosylation inhibitor) completely inhibited Phytophthora sojae growth, suggesting that N-glycosylation is necessary for oomycete development. We conducted a glycoproteomic analysis of P. sojae to identify and map all N-glycosylated proteins and to quantify differentially expressed glycoproteins associated with mycelia, asexual cysts, and sexual oospores. A total of 355 N-glycosylated proteins were found, containing 496 glycosites that likely participate in glycan degradation, carbon metabolism, glycolysis, or other central metabolic pathways. To verify the glycoproteomic results and further examine the function of N-glycosylation in P. sojae, two proteins were selected for PNGase F deglycosylation assays and CRISPR/Cas9-mediated site-directed mutagenesis, including a GPI transamidase protein (GPI16) up-regulated in cysts, with the consensus Nglyco-X-S/T motif at Asn 94, and a heat shock protein 70 (HSP70) up-regulated in cysts and oospores with a previously unknown Nglyco-N motif at Asn 270. We demonstrated that the GPI16 and HSP70 are both N-glycosylated proteins, confirming that the Nglyco-N motif is a target site for asparagine -oligosaccharide N-glycosidic linkage. Glycosite mutations of Asn 94 in the GPI16 led to impaired cyst germination and pathogenicity, while HSP70 mutants exhibited decreased cyst germination and oospore production. This work describes an integrated map of oomycete N-glycoproteomes and advances our understanding of N-glycosylation in oomycetes. Moreover, we confirm that the consensus Nglyco-X-S/T and the Nglyco-N -linked glycosites are both essential for the growth of Phytophthora sojae, indicating that there are multiple N-glycosylation motifs in oomycetes.

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PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae

Cited by 37 publications

References 53 publications

Phytophthora cinnamomi

Phytophthora cinnamomi

Colletotrichum higginsianum Mitogen-Activated Protein Kinase ChMK1: Role in Growth, Cell Wall Integrity, Colony Melanization, and Pathogenicity

The consensus N_glyco-X-S/T motif and a previously unknown N_glyco-N -linked glycosylation are necessary for growth and pathogenicity ofPhytophthora

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PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae

Cited by 37 publications

References 53 publications

Phytophthora cinnamomi

Phytophthora cinnamomi

Colletotrichum higginsianum Mitogen-Activated Protein Kinase ChMK1: Role in Growth, Cell Wall Integrity, Colony Melanization, and Pathogenicity

The consensus Nglyco-X-S/T motif and a previously unknown Nglyco-N -linked glycosylation are necessary for growth and pathogenicity ofPhytophthora

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The consensus N_glyco-X-S/T motif and a previously unknown N_glyco-N -linked glycosylation are necessary for growth and pathogenicity ofPhytophthora