PSORI-CM02 alleviates IMQ-induced mouse dermatitis via differentially regulating pro- and anti-inflammatory cytokines targeting of Th2 specific transcript factor GATA3

Wu, Dinghong; Zhang, Miaomiao; Li, Ning; Li, Xiong; Cai, Quan‐Wei; Yu, Wanlin; Liu, Liping; Zhu, Wei; Lu, Chuanjian

doi:10.1016/j.biopha.2018.11.092

Cited by 26 publications

(22 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…“0” represents none; “1” represents slight; “2” represents moderate; “3” represents marked; “4” represents very marked. The specific scoring criteria were described previously 39 .…”

Section: Methodsmentioning

confidence: 99%

Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8⁺ T-cell memory in wild-type and humanized mice

Chen¹,

Yan²,

Liu³

et al. 2020

Theranostics

View full text Add to dashboard Cite

Conventional immunosuppressants cause side effects and do not prevent the recurrence of autoimmune diseases. Moreover, they may not inhibit autoimmunity mediated by pathogenic memory T-cells. Dihydroartemisinin (DHA) has been shown to regulate autoimmunity. However, it remains unknown whether DHA impacts psoriasis and its recurrence. The objective of this study was to determine therapeutic effects of DHA on psoriasis and its relapse as well as its underlying mechanisms. Methods: We established animal models of imiquimod (IMQ)-induced psoriasis-like wild-type mice and humanized NSG mice receiving lesional human skin from patients with psoriasis. Many immunoassays, including immunohistochemistry, flow cytometry, quantitative RT-PCR and Western blotting, were performed. Results: We found that DHA not only ameliorated acute skin lesion of psoriatic mice, but also alleviated its recurrence by diminishing CD8 + central memory T (T CM ) and CD8 + resident memory T (T RM ) cells. It attenuated epidermal pathology and T-cell infiltration in the skin of IMQ-induced psoriatic mice while suppressing expression of IL-15, IL-17 and other proinflammatory cytokines in the skin. Surprisingly, DHA reduced the frequency and number of CD8 + , but not CD4 + , subset of CD44 high CD62L high T CM in psoriatic mice, whereas methotrexate (MTX) lowered CD4 + , but not CD8 + , T CM frequency and number. Indeed, DHA, but not MTX, downregulated eomesodermin (EOMES) and BCL-6 expression in CD8 + T-cells. Furthermore, DHA, but not MTX, reduced the presence of CD8 + CLA + , CD8 + CD69 + or CD8 + CD103 + T RM cells in mouse skin. Interestingly, treatment with DHA, but not MTX, during the first onset of psoriasis largely prevented psoriasis relapse induced by low doses of IMQ two weeks later. Administration of recombinant IL-15 or CD8 + , but not CD4 + , T CM cells resulted in complete recurrence of psoriasis in mice previously treated with DHA. Finally, we demonstrated that DHA alleviated psoriatic human skin lesions in humanized NSG mice grafted with lesional skin from psoriatic patients while reducing human CD8 + T CM and CD103 + T RM cells in humanized mice. Conclusion: We have provided the first evidence that DHA is advantageous over MTX in preventing psoriasis relapse by reducing memory CD8 + T-cells.

show abstract

Section: Methodsmentioning

confidence: 99%

Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8⁺ T-cell memory in wild-type and humanized mice

Chen¹,

Yan²,

Liu³

et al. 2020

Theranostics

View full text Add to dashboard Cite

show abstract

“…Materials and methods used in the studies had common grounds: many used BALB/c mice, had several days of imiquimod 5% applied on the hairless back skin and ear followed by topical curcumin ointments or gel applications. The evolution and involution of psoriatic lesions were evaluated by the PASI Score, Psoriasis Area and Severity Index which follows the erythema, scales and thickness of the affected skin (26,(31)(32)(33)(34).…”

Section: Resultsmentioning

confidence: 99%

“…Other experimental formulas and phytotherapeutics PSORI-CM01 and PSORI-CM02. PSORI-CM01 is the basis of the second formula and it has been proven through animal studies to have antipsoriatic properties by inducing autophagy and inhibiting the cytokines and chemokines involved in psoriasis (34,45).…”

Section: Resultsmentioning

confidence: 99%

“…PSORI-CM02 had no side effects in a study on BALB/c mice and it improved murine body mass (an important indicator for overall health and for imiquimod induced inflammation). This formula has immunomodulatory properties by reducing the T cell infiltrate, lowering the levels of interferon-γ, IL-17A and increasing the levels of IL-4, an anti-inflammatory agent, GATA3 pathway is an important therapeutic target (34).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Influence of phytochemicals in induced psoriasis (Review)

Niculeț

Radaschin

Năstase³

et al. 2020

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…PSORI-CM02 is an optimization formula of PSORI-CM01, a classic prescription of a national famous doctor Guo-Wei Xuan, which has been used for psoriasis vulgaris for more than twenty years in the Guangdong Provincial hospital [14]. PSORI-CM02 consists of Rhizoma curcumae, Radix paeoniae rubra, Sarcandra glabra, Rhizoma smilacis glabrae, and Fructus mume, which is proved to have anti-inflammatory effects and improve immunity [15]. RSG, which is a traditional Chinese medicine in PSORI-CM02, has been reported to have an anti-inflammatory effect, and its major effective active component is astilbin [16].…”

Section: Introductionmentioning

confidence: 99%

Study on Anti‐Inflammatory Effect and Major Anti‐Inflammatory Components of PSORI‐CM02 by Zebrafish Model

Yuan

Huang

Lei

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

PSORI-CM02 is an optimization formula of PSORI-CM01, which is a clinical herbal formula for the treatment for psoriasis in the Guangdong Provincial Hospital of Chinese Medicine. Previous research indicates that it plays a critical role in anti-inflammation and immunoregulation. Rhizoma smilacis glabrae (RSG) is one herbal medicine of PSORI-CM02, whose effective anti-inflammatory component is astilbin. This study aims to test the anti-inflammatory and immunoregulation effects of astilbin as well as RSG in PSORI-CM02, and we, respectively, used the CuSO4-induced neutrophil-specific transgenic zebrafish model Tg(mpx: EGFP) and the melanin allele mutated Albino strain zebrafish model to visualize the effects of neutrophil recruitment and macrophage phagocytosis. Our data indicated that both PSORI-CM02 and astilbin had anti-inflammatory effects, leading to a reduction in the recruitment of neutrophils and promotion in macrophage phagocytosis. Nevertheless, the negative liquor of Rhizoma smilacis glabrae (PSORI-CM02 without RSG) also had anti-inflammatory and promoting macrophage phagocytosis effects. The results revealed the formula excluding RSG also had anti-inflammatory and immunoregulation effects, which demonstrated that RSG was not the major anti-inflammatory herbal medicine in PSORI-CM02. Similarly, astilbin was not the major anti-inflammatory active ingredient in the formula. The anti-inflammatory and the promotion of macrophage phagocytosis effect of PSORI-CM02 in vivo zebrafish were the results of multiple component interaction, which was the common characteristic of the Chinese medicine compound.

show abstract

PSORI-CM02 alleviates IMQ-induced mouse dermatitis via differentially regulating pro- and anti-inflammatory cytokines targeting of Th2 specific transcript factor GATA3

Cited by 26 publications

References 29 publications

Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8⁺ T-cell memory in wild-type and humanized mice

Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8⁺ T-cell memory in wild-type and humanized mice

Influence of phytochemicals in induced psoriasis (Review)

Study on Anti‐Inflammatory Effect and Major Anti‐Inflammatory Components of PSORI‐CM02 by Zebrafish Model

Contact Info

Product

Resources

About

PSORI-CM02 alleviates IMQ-induced mouse dermatitis via differentially regulating pro- and anti-inflammatory cytokines targeting of Th2 specific transcript factor GATA3

Cited by 26 publications

References 29 publications

Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8+ T-cell memory in wild-type and humanized mice

Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8+ T-cell memory in wild-type and humanized mice

Influence of phytochemicals in induced psoriasis (Review)

Study on Anti‐Inflammatory Effect and Major Anti‐Inflammatory Components of PSORI‐CM02 by Zebrafish Model

Contact Info

Product

Resources

About

Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8⁺ T-cell memory in wild-type and humanized mice

Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8⁺ T-cell memory in wild-type and humanized mice