Psychromarinibacter sediminicola sp. nov., a novel salt-tolerant, metabolically diverse bacterium isolated from a solar saltern sediment, and comparison between members of family Roseobacteraceae

Abstract

Known for its species abundance and evolutionary status complexity, family Roseobacteraceae is an important subject of many studies on the discovery, identification, taxonomic status, and ecological properties of marine bacteria. In this study, six species from three genera (Psychromarinibacter, Lutimaribacter, and Maritimibacter) of family Roseobacteraceae were analyzed and compared, many intergeneric differences and intra-generic diversity were found in phylogenetic, genomic, biochemical, and chemo taxonomical properties. Moreover, a novel strain, named C21-152T was isolated from solar saltern sediment in Weihai, China. The values of 16S rRNA gene sequence similarity, the average nucleotide identity (ANI), and the digital DNA–DNA hybridization (dDDH) between genomes of the novel strain and P. halotolerans MCCC 1K03203T were 97.19%, 78.49%, and 21.90%, respectively. Genome sequencing revealed a complete Sox enzyme system related to thiosulfate oxidization as well as a complete pathway for the final conversion of hydroxyproline to α-ketoglutarate. In addition, strain C21-152T was resistant to many antibiotics and had the ability to survive under salt tolerance. This strain had versatile survival strategies in saline environments. Some of its physiological features enriched and complemented the knowledge of the characteristics of genus Psychromarinibacter. Optimum growth of strain C21-152T occurred at 37℃, with 5-6% (w/v) NaCl and at pH 7.5. According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain C21-152T should represent a novel specie of the genus Psychromarinibacter, for which the name Psychromarinibacter sediminicola sp. nov. is proposed. The type strain is C21-152T (=MCCC 1H00808T =KCTC 92746T =SDUM1063002T).