PTEN and p16 genes as epigenetic biomarkers in oral squamous cell carcinoma (OSCC): a study on south Indian population

Sushma, Pinninti Santosh; Jamil, Kaiser; Kumar, Parvinder; Satyanarayana, Uppala; Ramakrishna, Manasa; Triveni, B

doi:10.1007/s13277-015-4648-8

Cited by 27 publications

(26 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, 72.5% of OSCC have well differentiated grade, whereas moderately and poorly differentiated OSCC grade (III) constituted 17.5% and 10%, respectively. As reported in other studies, the majority of the patients presented with well or moderately differentiated tumors, most frequently located in the mobile tongue, floor of mouth and alveolar rim 35 . Alshawi (2010) 24 studied OSCC grading and revealed likewise percentages to our results (71.42% in grade I, 17.14% in grade II and 11.41 % in grade III).…”

Section: Discussionsupporting

confidence: 78%

“…The p16 INK4A gene is located on chromosome 9p21, and as a tumor suppressor gene, playing a key role in cell cycle regulation. It codes for a protein which binds and inhibits cyclin-dependent kinases (CdK-4and -6) 35 . It also inhibits phosphorylation of Rb and promotes the formation of an Rb-E2F repressive transcriptional complex, so as to blocks the progression of cell cycle at the G1-S point.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Molecular Implicatory Propositioning Roles for Human Cytomegalovirus and P16 Gene Expression in Oral Squamous Cellular Carcinogenesis

Sachit¹,

AL-Mahbobi²,

Ali³

et al. 2019

J Pure Appl Microbiol

View full text Add to dashboard Cite

Oral carcinogenesis is a multi-step process, affecting predominantly elderly males with a long history of smoking and alcohol drinking while the role of infectious agents in its progression has not been fully identified. Human cytomegalovirus (HCMV) has been hypothesized in the oral pathogenesis and carcinogenesis. Usually, p16 protein is absent in oral squamous cell carcinoma (OSCC), either for p16 gene being mutated or deleted or its expression being abrogated while p16 expression is associated with favorable prognosis. Is to analyze the concordant impact of p16 expression and HCMV infection on tissues from a group of patients with Oral squamous cell carcinoma (OSCC) in mid-Euphrates-Iraq. One-hundred formalin-fixed, paraffin-embedded Oral tissues enrolled in this study; 40 oral tissuebiopsies from Oral squamous cell carcinoma (OSCC); 40 tissues obtained from patients with benign oral tumors and 20 apparently healthy oral tissues were used as control group. Detection of HCMV was done by chromogenic in situ hybridization (CISH) technique while the protein expression of p16 gene by using immunohistochemistry (IHC) detection system. In OSCC group, the hCMV-positive CISH and the p16-positive IHC reactions were detected in 42.5% and.52.5% of tissues, respectively. The correlation between HCMV and P16 was highly significant (P= 0.003). The significant co-expression of P16 and HCMV genes in OSCC tissues could point for their possible role in either oral pathogenesis or carcinogenesis.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

A Molecular Implicatory Propositioning Roles for Human Cytomegalovirus and P16 Gene Expression in Oral Squamous Cellular Carcinogenesis

Sachit¹,

AL-Mahbobi²,

Ali³

et al. 2019

J Pure Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Briefly, 1 μg genomic DNA was modified with bisulfite using a CpGenome DNA modification kit (Chemicon, Temecula, CA, USA). The modified DNA was amplified with primers for the methylated and unmethylated gene sequences designed according previously published reports (Table S1) . The reactions were carried out using the Veriti 96‐well thermal cycler (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

“…The modified DNA was amplified with primers for the methylated and unmethylated gene sequences designed according previously published reports (Table S1). (25)(26)(27)(28) The reactions were carried out using the Veriti 96-well thermal cycler (Applied Biosystems). Polymerase chain reaction conditions are listed in Table S2.…”

Section: Methodsmentioning

confidence: 99%

Peperomin E reactivates silenced tumor suppressor genes in lung cancer cells by inhibition of DNA methyltransferase

et al. 2016

View full text Add to dashboard Cite

Advanced lung cancer has poor prognosis owing to its low sensitivity to current chemotherapy agents. Therefore, discovery of new therapeutic agents is urgently needed. In this study, we investigated the antitumor effects of peperomin E, a secolignan isolated from Peperomia dindygulensis, a frequently used Chinese folk medicine for lung cancer treatment. The results indicate that peperomin E has antiproliferative effects, promoting apoptosis and cell cycle arrest in non‐small‐cell lung cancer (NSCLC) cell lines in a dose‐dependent manner, while showing lower toxicity against normal human lung epidermal cells. Peperomin E inhibited tumor growth in A549 xenograft BALB/c nude mice without significant secondary adverse effects, indicating that it may be safely used to treat NSCLC. Furthermore, the mechanisms underlying the anticancer effects of peperomin E have been investigated. Using an in silico target fishing method, we observed that peperomin E directly interacts with the active domain of DNA methyltransferase 1 (DNMT1), potentially affecting its genome methylation activity. Subsequent experiments verified that peperomin E decreased DNMT1 activity and expression, thereby decreasing global methylation and reactivating the epigenetically silenced tumor suppressor genes including RASSF1A,APC,RUNX3, and p16INK4, which in turn activates their mediated pro‐apoptotic and cell cycle regulatory signaling pathways in lung cancer cells. The observations herein report for the first time that peperomin E is a potential chemotherapeutic agent for NSCLC. The anticancer effects of peperomin E may be partly attributable to its ability to demethylate and reactivate methylation‐silenced tumor suppressor genes through direct inhibition of the activity and expression of DNMT1.

show abstract

“…Moreover, the authors suggested that epigenetic changes in these genes might represent a valuable biomarker for the early detection of OSCC. 59 Similarly, the expression of γ-synuclein (SNCG) is related to the development of various carcinomas, including OSCC (Table 1). In their study, Cheng et al examined a correlation between DNA methylation in the SNCG gene and the development of OSCC, including the clinical symptoms of this malignancy.…”

Section: Oral Squamous Cell Carcinomamentioning

confidence: 99%

Diseases of the oral cavity in light of the newest epigenetic research: Possible implications for stomatology

2018

View full text Add to dashboard Cite

Epigenetics is the study of inheritable changes in gene expression without changes in the underlying deoxyribonucleic acid (DNA) sequence. The main mechanisms of epigenetic regulation include DNA methylation, modifications in histones, and micro-ribonucleic acids (miRNA). Recent research evidence has shown that environmental and lifestyle factors dynamically interact with the genome, influencing epigenetic changes, from development to the later stages of life. This happens across a spectrum, from physiological to pathological conditions, such as genetic defects, developmental disorders, infectious or inflammatory processes, cancers, mental disorders, and substance abuse. Epigenetic studies have been conducted in various medical disciplines (e.g., oncology, internal medicine or psychiatry), adding valuable insight to standard medical approaches. However, in stomatology, epigenetic research is still in its infancy; thus, this review is aimed at presenting the role of epigenetic mechanisms in diseases of the oral cavity, including periodontal diseases, caries, developmental anomalies, and oral carcinoma. In addition, this paper reveals new insights into epigenetic biomarkers that can be helpful in the detection, early diagnosis, prognosis, and treatment of different oral diseases. Moreover, this review is focused on the possible clinical implications (diagnostic and therapeutic) of epigenetics, in the form of some noninvasive methods that can possibly be used in the future for the screening, work-up, outcome prediction and novel treatments of some dental diseases. Finally, this paper highlights that an epigenetic approach can be useful for designing novel interventions that will improve the management of oral malignancies or developmental abnormalities.

show abstract

PTEN and p16 genes as epigenetic biomarkers in oral squamous cell carcinoma (OSCC): a study on south Indian population

Cited by 27 publications

References 32 publications

A Molecular Implicatory Propositioning Roles for Human Cytomegalovirus and P16 Gene Expression in Oral Squamous Cellular Carcinogenesis

A Molecular Implicatory Propositioning Roles for Human Cytomegalovirus and P16 Gene Expression in Oral Squamous Cellular Carcinogenesis

Peperomin E reactivates silenced tumor suppressor genes in lung cancer cells by inhibition of DNA methyltransferase

Diseases of the oral cavity in light of the newest epigenetic research: Possible implications for stomatology

Contact Info

Product

Resources

About