Pterocidin, a Cytotoxic Compound from the Endophytic Streptomyces hygroscopicus

Igarashi, Yasuhiro; Miura, Shin-Suke; Fujita, Tsuyoshi; Furumai, Tamotsu

doi:10.1038/ja.2006.28

Cited by 47 publications

(24 citation statements)

References 17 publications

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“…IR spectrum was measured on a Perkin Elmer Spectrum 100 (Perkin-Elmer, Fremont, CA, USA). NMR spectra were obtained on a Bruker AVANCE 400 or a Bruker AVANCE 500 spectrometer (Bruker, Rheinstetten, Germany) in DMSO-d 6 , referenced to residual solvent signals (d 2.49 for 1 H; d 39.5 for 13 C). HR-ESI-TOF-MS was recorded on a Bruker microTOF focus.…”

Section: Experimental Procedures General Experimental Proceduresmentioning

confidence: 99%

“…2 Meanwhile, it is quite notable that further discovery of unknown metabolites from Streptomyces is predicted by the genome analysis: the number of metabolites actually isolated is far more below the number of secondary metabolite biosynthetic gene clusters identified in the whole genomes of S. avermitilis and S. coelicolor. 3,4 As a part of our chemical investigation on microbial secondary metabolites, we reported plant-growth promoting spiroacetals of polyketide origin, 5 a linear polyketide with a dlactone terminus with cytotoxic activity, 6 a polycyclic tetronate with antiinvasive activity 7 and a biosynthetically unprecedented heterocyclic polyketide with antibacterial and antiinvasive activities 8 from Streptomyces. During the course of our continuing effort to discover structurally unique secondary metabolites from these organisms, a new modified peptide was isolated from the culture broth of a soilderived actinomycete strain Streptomyces sp.…”

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confidence: 99%

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Prajinamide, a new modified peptide from a soil-derived Streptomyces

et al. 2012

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Actinomycetes are well recognized as the richest source of bioactive compounds, including clinically important antibiotics, antitumor agents and cell function modulators, and hence of high pharmacological and commercial interest. 1 Amongst this group, members of the genus Streptomyces are the most prolific producers of secondary metabolites, accounting for up to 80% of the bioactive small molecules discovered from actinomycetes. 2 Meanwhile, it is quite notable that further discovery of unknown metabolites from Streptomyces is predicted by the genome analysis: the number of metabolites actually isolated is far more below the number of secondary metabolite biosynthetic gene clusters identified in the whole genomes of S. avermitilis and S. coelicolor. 3,4 As a part of our chemical investigation on microbial secondary metabolites, we reported plant-growth promoting spiroacetals of polyketide origin, 5 a linear polyketide with a dlactone terminus with cytotoxic activity, 6 a polycyclic tetronate with antiinvasive activity 7 and a biosynthetically unprecedented heterocyclic polyketide with antibacterial and antiinvasive activities 8 from Streptomyces. During the course of our continuing effort to discover structurally unique secondary metabolites from these organisms, a new modified peptide was isolated from the culture broth of a soilderived actinomycete strain Streptomyces sp. SPMA113 collected in Thailand. The strain was cultured in A-11M liquid medium, and the whole culture broth was extracted with 1-butanol. The HPLC/UV analysis of the extract using our in-house metabolite database indicated the presence of an unknown compound showing a UV absorption maximum at 260 nm, along with geldanamycins 9 and elaiophylins. 10

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Section: Experimental Procedures General Experimental Proceduresmentioning

confidence: 99%

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confidence: 99%

Prajinamide, a new modified peptide from a soil-derived Streptomyces

et al. 2012

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“…Streptomyces hygroscopicus is a species which produces a variety of antibiotics belonging to different classes with distinct structure and possessing certain biological properties [5,7,[10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Production Medium for Biosynthesis of Antifungal Antibiotic Ak-111-81 by Phosphate-Deregulated Mutant of Streptomyces hygroscopicus

Gesheva

2009

Appl Biochem Biotechnol

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Experimental mathematical designs were applied for optimization of a nutrient medium for biosynthesis of the antifungal antibiotic AK-111-81 by phosphate-deregulated mutant of Streptomyces hygroscopicus 111-81. Antifungal antibiotic AK-111-81 possesses well-expressed activity against Fusarium graminearum and other phytopathogenic fungi. The level of the production of the antibiotic AK-111-81 on this medium is more than three times higher than on the initial medium. The optimized quantitative composition of the nutrient culture media is (g/l): glucose, 20; soy meal, 18; ammonium succinate, 3; CaCO(3), 1.

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“…Igarashi et al, 2006;Yamada et al, 2012) or produced milbemycin oxime, which was shown to be toxic to mice (Xu et al, 2010).…”

Section: Minireview 3 Evaluating Genetic Stability Of Microorganismsmentioning

confidence: 99%

Literature search and data collection on RA for human health for microorganisms used as plant protection products

Hackl

Pacher‐Zavisin

Sedman

et al. 2015

EFSA Supporting Publications

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A knowledge base of scientific and regulatory data relevant in the context of risk assessment of microbial plant protection products (PPPs) for human health was established, taking into consideration conditions 1 to 4 in Regulation (EU) 546/2011 that must be met before authorisation can be granted. We screened scientific publications and documents from governmental and institutional sources and extracted relevant information into a database, performed comparative genome analyses to explore possibilities and limitations of applying read-across among PPP strains, and discussed the collective findings among a group of experts in relevant fields. Besides information on factors relevant for the evaluation of microbial PPP strains regarding pathogenicity, infectivity, and toxicity, information was collected on methods used to analyse the PPP species' ability to persist and grow in test animals, for detecting microbial DNA transfer, for testing microbial compounds for potential toxicity, and for detecting toxin production from PPP strains. Questions arising from the reviewed scientific and grey literature were how to address the specific biological properties of PPP strains and how to take into consideration realistic exposure scenarios and a natural background of environmental microorganisms in the risk assessment. Results from the present work imply that pathogenicity traits have to be considered as strain specific and that, hence, read-across comparison cannot be used. From a scientific point of view, it was highly recommended to provide whole genome sequencing data of PPP strains to allow their unambiguous identification, while other technological developments such as the use of metabolomics data were seen as an option for future implementation. It is envisaged to further develop the key topics of the present work at a joint stakeholder workshop as the next step towards the preparation of a guidance document by the PPR panel on how to conduct the regulatory risk assessment for microbial pesticides. © Efsa 2015Key words: microorganism, pesticide, risk assessment, human health, toxicity, read-across, DNA sequencing Disclaimer: The present document has been produced and adopted by the bodies identified above as authors. This task has been carried out exclusively by the authors in the context of a contract between the European Food Safety Authority and the authors, awarded following a tender procedure. The present document is published complying with the transparency principle to which the Authority is subject. It may not be considered as an output adopted by the Authority. The European Food Safety Authority reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors.Suggested citation: Evelyn Hackl1), Margit Pacher-Zavisin2), Laura Sedman1), Stefan Arthaber1), Ulla Bernkopf1), Günter Brader1), Markus Gorfer1), Birgit Mitter1), Aspasia Mitropoulou1), Monika Schmoll1), Willem van Hoesel1), Elisabeth Wis...

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Pterocidin, a Cytotoxic Compound from the Endophytic Streptomyces hygroscopicus

Cited by 47 publications

References 17 publications

Prajinamide, a new modified peptide from a soil-derived Streptomyces

Prajinamide, a new modified peptide from a soil-derived Streptomyces

Optimization of the Production Medium for Biosynthesis of Antifungal Antibiotic Ak-111-81 by Phosphate-Deregulated Mutant of Streptomyces hygroscopicus

Literature search and data collection on RA for human health for microorganisms used as plant protection products

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