The importance of road safety in the developmental agenda of a country cannot be overemphasized. It is essential for effective socioeconomic development. However, several countries in the developing world are confronted by several challenges relating to road safety, which are inadequately investigated. These challenges further aggravate the already heavily burdened health-care systems. The aim of this study is, therefore, to determine and analyse road safety issues in Ghana aimed at contributing to national policy development, stakeholder engagements, and public safety education campaigns on road traffic collision. A qualitative study by one-on-one interviews with individuals (n = 97) in road safety leadership positions was performed from November 2018 to February 2019. The interviews were audio-recorded and transcribed. Data analysis was conducted using a constant comparative methodology approach facilitated by Atlas.ti 8.0 software. Important road safety challenges that were identified by the respondents were categorized into six thematic areas, namely, institutional, executional, managerial and operational, attitudinal and behavioural, research, and financial and investment challenges. We recommend that the government and stakeholders alike should tackle these challenges by building a collaborative environment where everyone is involved in the process of developing and implementing strategies aimed at overcoming these challenges as they arise. There is also the need to address the epidemic carnage of road traffic injuries, many of which are preventable since they arise from human actions and inactions.