Puerarin Improves Dexamethasone-Impaired Wound Healing In Vitro and In Vivo by Enhancing Keratinocyte Proliferation and Migration

Abstract

The delayed and impaired wound healing caused by dexamethasone (DEX) is commonly reported. Puerarin, the major isoflavone found in Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep promoted the wound healing process in diabetic rats. However, the effects and underlying mechanisms of puerarin on DEX-impaired wound healing have not been investigated. This study examined the potential uses of puerarin in upregulating keratinocyte proliferation and migration in dexamethasone (DEX)-suppressed wound healing model. The effects of puerarin on wound healing in vivo were investigated by taking full-thickness 5 mm punch biopsies from the dorsal skin of BALB/c mice and then treating them topically with 0.1% DEX. For the in vitro study, DEX-treated HaCaT cells were used to examine the effects of puerarin on DEX-induced keratinocyte proliferation and migration and the mechanisms of its action. Puerarin, when applied topically, accelerated the wound closure rate, increased the density of the capillaries, and upregulated the level of collagen fibers and TGF-β in the wound sites compared to the DEX-treated mice. Puerarin promoted the proliferation and migration of keratinocytes by activating the ERK and Akt signaling pathways in DEX-treated HaCaT cells. In conclusion, puerarin could be effective in reversing delayed and disrupted wound healing associated with DEX treatments.

Oral administration of sea cucumber (Stichopus japonicus) protein exerts wound healing effectsviathe PI3K/AKT/mTOR signaling pathway

“…Re-epithelialization was achieved through cell migration of keratinocytes. 28 In this study, epidermal thickness around the wound was observed by H&E staining, as shown in Fig. 3A.…”

“…In the hemostatic phase, some growth factors were released to accelerate skin resurfacing and neovascularization, such as transforming growth factors. 28 The formation of collagen fibers cross-linking also enhanced the tensile strength of the tissue. 30,31 Hence, SCP has a beneficial capacity for cell and tissue proliferation.…”

Synthesis of Biogenic Gold Nanoparticles by Using Sericin Protein from Bombyx mori Silk Cocoon and Investigation of Its Wound Healing, Antioxidant, and Antibacterial Potentials

“…The effects of SSP-AuNPs on the viability of HaCaT cells were examined using MTT assays. 34 HaCaT cells were seeded at a density of 5×10 4 cells/well to 96-well plates. After 24 h, cells were treated with SSP-AuNPs (5, 10, 25 µg/mL) for 24 h. After treatment, the culture medium was removed, then 100 µL fresh medium and 10 µL of MTT reagent (5 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) were added to each well.…”

“…For scratch wound assay of SSP-AuNPs, the HaCaT cells were seeded at a density of 4×10 5 cells/well on 12-well plates and allowed to achieve 100% confluence. 34 , 35 Then, the cells were scratched and treated with SSP-AuNPs samples (5, 10, 25 µg/mL) or Centella asiatica extract (positive control, 100 µg/mL; United States Pharmacopeia (USP) Reference Standard, purchased from Sigma Aldrich). The images of scratch gap width for each time-point were captured by Lionheart FX Automated imaging system and measured using Gen5 Imager software (Biotek Instruments Inc., Winooski, VT, USA).…”

Pharmacological Activity, Pharmacokinetics, and Clinical Research Progress of Puerarin

“…Puerarin promotes keratinocyte proliferation and migration by activating ERK and Akt signaling pathways in DEX-treated HaCaT cells. In a nutshell, puerarin effectively reversed the deferred and interrupted wound healing connected with to the DEX treatment mechanism [ 126 ]. Puerarin can prevent LPS-induced vascular endothelial injury, and the mechanism may be connected with the restraint of NF-κB activation, then changing the levels of inflammatory factors and clotting-relevant factors [ 127 ].…”