Puerarin improves OVX-induced osteoporosis by regulating phospholipid metabolism and biosynthesis of unsaturated fatty acids based on serum metabolomics

Li, Bo; Wang, Yu; Gong, Shiqiang; Yao, Weifan; Gao, Hua; Liu, Mingyan

doi:10.1016/j.phymed.2022.154198

Cited by 33 publications

(4 citation statements)

References 28 publications

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“…Additionally, a multiomics study in a large cohort identified an amino acid metabolism-mediated association between gut microbiota and osteoporosis, suggesting that gut dysbiosis and amino acid metabolism could be a target for osteoporosis treatment ( Ling et al., 2021 ). Recent research suggested that puerarin may promote the biosynthesis of unsaturated fatty acids and regulate phospholipid metabolism in OVX-induced osteoporosis ( Li B. et al., 2022 ), and the results showed that biosynthesis of unsaturated fatty acids was important in undermining adipogenic differentiation and promoting osteogenic differentiation of BMSCs (bone marrow mesenchymal stem cells) in ovariectomized rats. Although the relationship between steroid hormones and osteoporosis has long been established, relevant research has indicated that probiotics can prevent sex steroid deficiency-induced bone loss ( Li et al., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of the gut microbiota and serum metabolites in postmenopausal women with reduced bone mineral density

Yan,

Wang,

et al. 2024

Front. Cell. Infect. Microbiol.

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IntroductionEmerging evidence suggests that the gut microbiota is closely associated with bone homeostasis. However, little is known about the relationships among the bone mineral density (BMD) index, bone turnover markers, and the gut microbiota and its metabolites in postmenopausal women.MethodsIn this study, to understand gut microbiota signatures and serum metabolite changes in postmenopausal women with reduced BMD, postmenopausal individuals with normal or reduced BMD were recruited and divided into normal and OS groups. Feces and serum samples were collected for 16S rRNA gene sequencing, liquid chromatography coupled with mass spectrometry (LC-MS)-based metabolomics and integrated analysis.ResultsThe results demonstrated that bacterial richness and diversity were greater in the OS group than in the normal group. Additionally, distinguishing bacteria were found among the two groups and were closely associated with the BMD index and bone turnover markers. Metabolomic analysis revealed that the expression of serum metabolites, such as etiocholanolone, testosterone sulfate, and indole-3-pyruvic acid, and the corresponding signaling pathways, especially those involved in tryptophan metabolism, fatty acid degradation and steroid hormone biosynthesis, also changed significantly. Correlation analysis revealed positive associations between normal group-enriched Bacteroides abundance and normal group-enriched etiocholanolone and testosterone sulfate abundances; in particular, Bacteroides correlated positively with BMD. Importantly, the tryptophan-indole metabolism pathway was uniquely metabolized by the gut bacteria-derived tnaA gene, the predicted abundance of which was significantly greater in the normal group than in the control group, and the abundance of Bacteroides was strongly correlated with the tnaA gene.DiscussionOur results indicated a clear difference in the gut microbiota and serum metabolites of postmenopausal women. Specifically altered bacteria and derived metabolites were closely associated with the BMD index and bone turnover markers, indicating the potential of the gut microbiota and serum metabolites as modifiable factors and therapeutic targets for preventing osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Characteristics of the gut microbiota and serum metabolites in postmenopausal women with reduced bone mineral density

Yan,

Wang,

et al. 2024

Front. Cell. Infect. Microbiol.

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“…Due to relative safety, natural products and herbs grab increasing attention to develop new anti-osteoporosis drugs. [8] Lycii Fructus also called Gouqizi is the fruit of plant Lycium barbarum, and was firstly described in Sheng Nong's herbal classic as a Chinese kidney-tonifying herb. [9] Also, Lycii Fructus is widely used in home cooking such as tea, soups and porridge for its Chinese Pharmacopeia and pleasant flavor.…”

Section: Introductionmentioning

confidence: 99%

“…Due to relative safety, natural products and herbs grab increasing attention to develop new anti-osteoporosis drugs. [ 8 ]…”

Section: Introductionmentioning

confidence: 99%

Network pharmacology-based pharmacological mechanism prediction of Lycii Fructus against postmenopausal osteoporosis

Wang,

et al. 2023

Medicine

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Postmenopausal osteoporosis (PMOP) has become one of most frequent bone diseases worldwide with aging population. Lycii Fructus, a common plant fruit with the property of drug homologous food, has long since been used to treat PMOP. The aim of this study is to explore pharmacological mechanisms of Lycii Fructus against PMOP through using network pharmacology approach. The active ingredients of Lycii Fructus were obtained from Traditional Chinese Medicine System Pharmacology database. Target fishing was performed on these ingredients in UniProt database for identification of the relative targets. Then, we screened the targets related to PMOP using GeneCards database and DisGeNET database. The overlapping genes between PMOP and Lycii Fructus were obtained to perform protein–protein interaction, gene ontology analysis, Kyoto Encyclopedia of Genes and Genomes analysis. A total of 35 active ingredients were identified in Lycii Fructus, and fished 158 related targets. Simultaneously, 292 targets associated with PMOP were obtained from GeneCards database and DisGeNET database. By drawing Venn diagram, 41 overlapping genes were obtained, and were considered as therapeutically relevant. Gene ontology enrichment analysis predicted that anti-inflammation and promotion of angiogenesis might be 2 potential mechanism of Lycii Fructus for PMOP treatment. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed several pathways, such as IL-17 pathway, TNF pathway, MAPK pathway, PI3K-Akt signaling pathway and HIF signaling pathway were involved in regulating these 2 biological processes. Through the method of network pharmacology, we systematically investigated the mechanisms of Lycii Fructus against PMOP. The identified multi-targets and multi-pathways provide new insights to further determinate its exact pharmacological mechanisms.

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“…By enhancing osteogenesis and promoting bone formation, PUE also improves OVX-induced osteoporosis and lipid metabolism by regulating phospholipid metabolism and polyunsaturated fatty acid biosynthesis, thereby reducing adipogenic differentiation. In addition, activation of the Wnt pathway and inhibition of the PPARγ pathway promote adipogenesis in osteogenic differentiation of inactivated rat bone marrow mesenchymal stem cells ( Li et al, 2022 ). For osteoporosis caused by postmenopausal estrogen deficiency, the results of a clinical trial showed that PUE was well tolerated for the short-term treatment of mild to severe menopausal symptoms in women.…”

Section: Introductionmentioning

confidence: 99%

A mechanistic review of chinese medicine polyphenols on bone formation and resorption

Li²,

Li³

et al. 2022

Front. Pharmacol.

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Bone reconstruction includes a steady state system of bone formation and bone absorption. This tight coupling requires subtle coordination between osteoblasts and osteoclasts. If this balance is broken, it will lead to bone mass loss, bone density reduction, and bone metabolic diseases, such as osteoporosis. Polyphenols in Chinese herbal medicines are active ingredients in plant extracts with high safety and few side effects, and they can play a role in affecting bone formation and bone resorption. Some of these have estrogen-like effects and can better target bone health in postmenopausal women. The purpose of this review is to provide comprehensive information on the mechanisms underlying the relationship between traditional Chinese medicine polyphenols and bone formation or bone resorption.

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Puerarin improves OVX-induced osteoporosis by regulating phospholipid metabolism and biosynthesis of unsaturated fatty acids based on serum metabolomics

Cited by 33 publications

References 28 publications

Characteristics of the gut microbiota and serum metabolites in postmenopausal women with reduced bone mineral density

Characteristics of the gut microbiota and serum metabolites in postmenopausal women with reduced bone mineral density

Network pharmacology-based pharmacological mechanism prediction of Lycii Fructus against postmenopausal osteoporosis

A mechanistic review of chinese medicine polyphenols on bone formation and resorption

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