Puerarin protects against cadmium-induced proximal tubular cell apoptosis by restoring mitochondrial function

Song, Xiang-Bin; Liu, Gang; Wang, Zhenyong; Wang, Lin

doi:10.1016/j.cbi.2016.10.006

Cited by 44 publications

(31 citation statements)

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“…Parthanatos is reported that it is related to a variety of diseases, such as diabetes, inflammation, Brain trauma and so on 30, 31 . An important feature is the dependence on the PARP-1 protein, and previous study found that Cd treatment of renal tubular epithelial cells could increase the expression of PARP proteins significantly 32 . In addition, it was reported that PARP-1 protein activity accounts for almost 90% of the activity of the PARP protein family 33 .…”

Section: Discussionmentioning

confidence: 99%

PARP-1 overexpression contributes to Cadmium-induced death in rat proximal tubular cells via parthanatos and the MAPK signalling pathway

Luo

Yuan

et al. 2017

Sci Rep

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Parthanatos is a newly discovered form of PARP-1-dependent programmed cell death. It has been reported to play an important role in several cancer or tumour cells; however, few studies have been performed in normal cells. Cadmium is a highly toxic pollutant and is reported to induce autophagy and apoptosis in multiple cell types. Although cadmium toxicity induces cell death, the underlying mechanism is not fully understood. Therefore, in this study we aimed to investigate the mechanism of Cadmium -induced cell damage using rat proximal tubular cell line NRK-52E and primary rat proximal tubular (rPT) cells. Our results indicated that parthanatos and the MAPK signalling pathway contribute to Cadmium-induced cell death, and that oxidative stress and mitochondrial damage play key roles in this process. In addition, parthanatos with oxidative stress has a synergistic effect on apoptosis, and JNK1/2 and p38 contribute to parthanatos.

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Section: Discussionmentioning

confidence: 99%

PARP-1 overexpression contributes to Cadmium-induced death in rat proximal tubular cells via parthanatos and the MAPK signalling pathway

Luo

Yuan

et al. 2017

Sci Rep

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“…Puerarin([8‐(β‐ d ‐glucopyranosyl‐7‐hydroxy‐3‐(4‐hydroxyphenyl)‐4H‐1‐benzopyran‐4‐one]) is a major bioactive isoflavone compound isolated from the roots of the Pueraria lobata and has been clinically used for its protection against inflammation, oxidative stress, and mitochondrial dysfunction (Wang, Shi, et al, ; Zeng et al, ). It is reported that puerarin might be beneficial to improve lipid metabolism (Prasain, Peng, Rajbhandari, & Wyss, ), restore mitochondrial function (Song, Liu, Wang, & Wang, ), and meliorate glucose homeostasis (Yang et al, ). Deng, Wang, Sun, and Xiao () indicated that puerarin could correct lipid metabolism disorder in endothelial cells via activation of PI3K/AKT pathway.…”

Section: Introductionmentioning

confidence: 99%

Puerarin protects against high‐fat high‐sucrose diet‐induced non‐alcoholic fatty liver disease by modulating PARP‐1/PI3K/AKT signaling pathway and facilitating mitochondrial homeostasis

Wang

Yang

Shang

et al. 2019

Phytotherapy Research

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As yet, there was no effective pharmacological therapy approved for non‐alcoholic fatty liver disease (NAFLD). Here, we aimed to evaluate the therapeutic potential of puerarin against NAFLD and explored the underlying mechanisms. C57BL/6J mice were fed with a high‐fat high‐sucrose (HFHS) diet with or without puerarin coadministration intragastrically. The levels of hepatocellular injury, steatosis, fibrosis, and mitochondrial and metabolism alteration were detected. First, puerarin ameliorated histopathologic abnormalities due to HFHS. We observed a marked increase in hepatic lipid content, inflammation, and fibrosis level, which were attenuated by puerarin. Possible mechanisms were related to puerarin‐mediated activation of PI3K/AKT pathway and further improvement in fatty acid metabolism. Puerarin restored the NAD+ content and beneficially affected the hepatic mitochondrial function, which attenuated HFHS‐induced steatosis and metabolic disturbances. Finally, hepatic PARP‐1 was activated due to excessive fat intake. Puerarin attenuated the PARP‐1 expression in HFHS‐fed mice, and PJ34, the PARP inhibitor, could mimic these protections of puerarin. However, pharmacological inhibition of PI3K disabled the protection of puerarin or PJ34 toward NAD+ refilling and mitochondrial homeostasis. In conclusion, our findings indicated that puerarin could be a promising and practical therapeutic strategy in NAFLD through modulating PARP‐1/PI3K/AKT signaling pathway and further facilitating mitochondrial function.

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“…The beneficial effects of puerarin on the various medicinal purposes may be due to its wide spectrum of pharmacological properties such as vasodilation, cardioprotection, neuroprotection, antioxidant, anticancer, anti-inflammation, alleviating pain, promoting bone formation, inhibiting alcohol intake, and attenuating insulin resistance [23]. Puerarin also had the ability to restore mitochondrial dysfunctions [18,24,25]. In this study, we found that puerarin increased mitochondrial biogenesis in osteoarthritis rats (Fig.…”

Section: Discussionmentioning

confidence: 57%

“…It has been described that mitochondrial biogenesis was impaired in osteoarthritis chondrocytes [13], while puerarin could restore mitochondrial function [18]. As puerarin protected rats from cartilage damages and osteoarthritis, we continued to investigate the effect of puerarin on mitochondrial biogenesis in osteoarthritis rats.…”

Section: Puerarin Treatment Enhanced Mitochondrial Biogenesis In Ostementioning

confidence: 98%

“…Puerarin has also been reported to possess different biological activities including anti-oxidation, anti-inflammation, anti-apoptosis, and neuroprotection [15][16][17]. Song et al [18] reported that puerarin can restore mitochondrial function and protect tubular cell from apoptosis. As mitochondrial dysfunction was associated with osteoarthritis while puerarin can restore mitochondrial function, we tested the potential protective role of puerarin in osteoarthritis.…”

Section: Introductionmentioning

confidence: 99%

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Puerarin Attenuates Osteoarthritis via Upregulating AMP-Activated Protein Kinase/Proliferator-Activated Receptor-γ Coactivator-1 Signaling Pathway in Osteoarthritis Rats

et al. 2018

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Background/Aims: Osteoarthritis is the most common degenerative joint disease and causes major pain and disability in adults. It has been reported that mitochondrial dysfunction in chondrocytes was associated with osteoarthritis. Puerarin has multiple effects including restoring mitochondrial function. In this study, the potential effects of puerarin on osteoarthritis and osteoarthritis associated mitochondrial dysfunctions were evaluated. Methods: Osteoarthritis rats were treated with puerarin and the severity of osteoarthritis and cartilage damages was evaluated. The mitochondrial biogenesis and functions were analyzed by measuring related proteins expression, mitochondrial DNA content, ATP production, and oxygen consumption. The dependence of AMP-activated protein kinase (AMPK) pathway on puerarin-regulated mitochondrial function was analyzed by applying AMPK inhibitor Compound C. Results: Puerarin treatment alleviated mechanical hyperalgesia and cartilage damage in osteoarthritis rats. Puerarin increased mitochondrial biogenesis and attenuated mitochondrial dysfunctions in osteoarthritis rats. AMPK inhibitor Compound C abolished puerarin’s effects. Conclusion: Puerarin attenuates osteoarthritis by upregulating the AMPK/proliferator-activated receptor-γ coactivator signaling pathway in osteoarthritis rats.

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Puerarin protects against cadmium-induced proximal tubular cell apoptosis by restoring mitochondrial function

Cited by 44 publications

References 44 publications

PARP-1 overexpression contributes to Cadmium-induced death in rat proximal tubular cells via parthanatos and the MAPK signalling pathway

PARP-1 overexpression contributes to Cadmium-induced death in rat proximal tubular cells via parthanatos and the MAPK signalling pathway

Puerarin protects against high‐fat high‐sucrose diet‐induced non‐alcoholic fatty liver disease by modulating PARP‐1/PI3K/AKT signaling pathway and facilitating mitochondrial homeostasis

Puerarin Attenuates Osteoarthritis via Upregulating AMP-Activated Protein Kinase/Proliferator-Activated Receptor-γ Coactivator-1 Signaling Pathway in Osteoarthritis Rats

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