Pufferfish poisoning from Arothron stellatus: The first confirmed case in India with exact DNA sequencing-based species identification

Joseph, Toms C.; Goswami, Dharmendra B.; Pradeep, M A; Anupama, T.K.; Parmar, Ejaz; Renuka, V.; Remya, S.; Ravishankar, C.N.

doi:10.1016/j.toxicon.2021.07.014

Cited by 3 publications

(1 citation statement)

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“…Pufferfish poisoning is typical of warm waters and was regarded as a problem confined to Asian countries, , including Thailand, Taiwan, Singapore, Cambodia, Bangladesh, and India. , However, toxic pufferfish species have expanded to other regions, and there have been an increasing number of reports of incidences in the Mediterranean Sea, which has been attributed to the opening of the Suez Canal (the “Lessepsian migration”), which resulted in the migration of species from the Red Sea to colonize the Mediterranean Sea, − the Aegean Sea, the Adriatic Sea, and Oman, and there have also been reports of the incidence of tetrodotoxin in Australia and the United States, highlighting the widespread distribution of the toxin.…”

Section: Introductionmentioning

confidence: 99%

Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish

et al. 2021

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The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expansion to nonendemic areas has increased the need for fast and reliable detection of the toxin to effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered the most accurate method, although competitive immunoassays have also been reported. In this work, we sought to develop an aptamer-based assay for the rapid, sensitive, and cost-effective detection of TTX in pufferfish. Using capture-SELEX combined with next-generation sequencing, aptamers were identified, and their binding properties were evaluated. Finally, a highly sensitive and user-friendly hybrid antibody−aptamer sandwich assay was developed with superior performance compared to several assays reported in the literature and commercial immunoassay kits. The assay was successfully applied to the quantification of TTX in pufferfish extracts, and the results obtained correlated very well with a competitive magnetic bead-based immunoassay performed in parallel for comparison. This is one of the very few works reported in the literature of such hybrid assays for small-molecule analytes whose compatibility with field samples is also demonstrated.

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