Pullulan-hyperproducing color variant strain of Aureobasidium pullulans FB-1 newly isolated from phylloplane of Ficus sp.

Singh, Ram Sarup; Saini, Gaganpreet K.

doi:10.1016/j.biortech.2007.08.003

Cited by 81 publications

(34 citation statements)

References 14 publications

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“…Several reports have been published on production of pullulan via fermentation (Jiang, 2010;Ravella et al, 2010;Singh & Saini, 2008). Recently, we have also reported high pullulan production (66.79 g L −1 ) using glucose, yeast extract and peptone as the substrate by an osmo-tolerant strain of A. pullulans RBF 4A3 (Choudhury, Saluja, & Prasad, 2011).…”

Section: Introductionmentioning

confidence: 92%

Utilization of corn steep liquor for biosynthesis of pullulan, an important exopolysaccharide

Sharma

Prasad

Choudhury

2013

Carbohydrate Polymers

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Section: Introductionmentioning

confidence: 92%

Utilization of corn steep liquor for biosynthesis of pullulan, an important exopolysaccharide

Sharma

Prasad

Choudhury

2013

Carbohydrate Polymers

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“…pullulans FB-1, an isolate of our laboratory, was used for the present work. The culture has been isolated, identified, and maintained as described earlier [21]. It has been deposited in Microbial Type Culture Collection (MTCC), Chandigarh, India and assigned accession no.…”

Section: Microorganismmentioning

confidence: 99%

Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1

Singh

Saini

2008

Appl Biochem Biotechnol

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Culture conditions for pullulan production by Aureobasidium pullulans were optimized using response surface methodology at shake flask level without pH control. In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the levels of five factors significantly affecting the pullulan production, biomass production, and sugar utilization in submerged cultivation. The selected factors included concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride. Using this methodology, the optimal values for concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride were 5.31%, 0.11%, 0.07%, 0.05%, and 0.15% (w/v), respectively. This optimized medium has projected a theoretically production of pullulan of 4.44%, biomass yield of 1.03%, and sugar utilization of 97.12%. The multiple correlation coefficient 'R' was 0.9976, 0.9761 and 0.9919 for pullulan production, biomass production, and sugar utilization, respectively. The value of R being very close to one justifies an excellent correlation between the predicted and the experimental data.

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“…The absorption around 850 cm -1 characterized the a-configuration of a-D-glucopyranose units [2]. , as reported earlier [34]. The medium ingredients were also performed FTIR analysis and exhibited no contribution to the absorbance mentioned previously.…”

Section: Ftir Of Pullulanmentioning

confidence: 94%

“…Sucrose is a favorable carbon source for pullulan production by A. pullulans [17,34]; however, some studies reported that excess sucrose (i.e., above 5%) will inhibit pullulan production and hence limit the application of a batch fermentation system [35]. Nitrogen source also plays a significant role on pullulan production.…”

Section: Introductionmentioning

confidence: 97%

Enhanced pullulan production in a biofilm reactor by using response surface methodology

Cheng

Demirci

Catchmark

2010

J Ind Microbiol Biotechnol

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Pullulan is a linear homopolysaccharide that is composed of glucose units and often described as alpha-1, 6-linked maltotriose. In this study, response surface methodology using Box-Behnken design was employed to study the effects of sucrose and nitrogen concentrations on pullulan production. A total of 15 experimental runs were carried out in a plastic composite support biofilm reactor. Three-dimensional response surface was generated to evaluate the effects of the factors and to obtain the optimum condition of each factor for maximum pullulan production. After 7-day fermentation with optimum condition, the pullulan production reached 60.7 g/l, which was 1.8 times higher than the result from initial medium, and was the highest yield reported to date. The quality analysis demonstrated that the purity of produced pullulan was 95.2%, and its viscosity was 2.5 centipoise (cP), which is higher than the commercial pullulan and related to its molecular weight. Fourier transform infrared spectroscopy (FTIR) also suggested that the produced exopolysaccharide was pullulan.

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Pullulan-hyperproducing color variant strain of Aureobasidium pullulans FB-1 newly isolated from phylloplane of Ficus sp.

Cited by 81 publications

References 14 publications

Utilization of corn steep liquor for biosynthesis of pullulan, an important exopolysaccharide

Utilization of corn steep liquor for biosynthesis of pullulan, an important exopolysaccharide

Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1

Enhanced pullulan production in a biofilm reactor by using response surface methodology

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