Pulmonary Disease Associated With Nonencapsulated Streptococcus pneumoniae

Martin, Caleb; Bradshaw, Jessica L.; Pipkins, Haley R.; McDaniel, Larry S.

doi:10.1093/ofid/ofy135

Cited by 11 publications

(5 citation statements)

References 15 publications

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“…Among these genes, cpsA is a conserved pneumococcal capsular polysaccharide gene, and aliB‐like ORF1 and aliB‐like ORF2 are frequently present in the capsular region of non‐capsulated pneumococci. PCR were performed following the protocol described in previous reports 15,17,18 …”

Section: Methodsmentioning

confidence: 99%

Identification and molecular epidemiology of routinely determined Streptococcus pneumoniae with negative Quellung reaction results

Jia

Shi

Dong

et al. 2022

Clinical Laboratory Analysis

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Background: Some streptococci strains identified as Streptococcus pneumoniae (S. pneumoniae) by routine clinical methods exhibiting negative Quellung reaction results may belong to other species of viridans group streptococci or non-typeable S. pneumoniae. The purpose of this study was to investigate the identification and molecular characteristics of S. pneumoniae with negative Quellung reaction results.Methods: One hundred and five isolates identified as S. pneumoniae using routine microbiological methods with negative Quellung reaction results were included.Multilocus sequence analysis (MLSA) was used as a gold standard in species identification, and the capacity of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in identification was evaluated. Capsular genes and sequence types of S. pneumoniae isolates were determined by sequential multiplex PCR and multilocus sequence typing. Antimicrobial susceptibility patterns were determined via broth microdilution with a commercialized 96-well plate.Results: Among the isolates, 81 were identified as S. pneumoniae and 24 were S. pseudopneumoniae by MLSA. MALDI-TOF MS misidentified six S. pneumoniae isolates as S. pseudopneumoniae and nine S. pseudopneumoniae isolates as S. pneumoniae or S. mitis/S. oralis. Thirty-one sequence types (STs) were detected for these 81 S. pneumoniae isolates, and the dominant ST was 19.8%). The non-susceptibility rates of S. pseudopneumoniae were comparable to those of NESp strains.Conclusions: Some S. pneumoniae isolates identified by routine methods were S. pseudopneumoniae. Most NESp strains have a different genetic background compared with capsulated S. pneumoniae strains. The resistance patterns of S. pseudopneumoniae against common antibiotics were comparable to those of NESp.

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Section: Methodsmentioning

confidence: 99%

Identification and molecular epidemiology of routinely determined Streptococcus pneumoniae with negative Quellung reaction results

Jia

Shi

Dong

et al. 2022

Clinical Laboratory Analysis

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“…Moreover, NP Sp is underestimated because the typing clinical tests for characterization are based on the capsule. NP Sp strains are responsible for non-invasive diseases such as AOM [ 19 ], non-bacteremic pneumonia [ 20 ] and conjunctivitis [ 21 , 22 , 23 ] and rarely produce IPD [ 24 , 25 ]. On the other hand, it is common for many of these strains to show resistance to antibiotics [ 18 , 26 , 27 ], and they can participate in the global pathogenesis as a reservoir of resistance genes that can be transferred to capsulated strains [ 28 ].…”

Section: Streptococcus Pneumoniae Pathogenesismentioning

confidence: 99%

Pneumococcal Choline-Binding Proteins Involved in Virulence as Vaccine Candidates

et al. 2021

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Streptococcus pneumoniae is a pathogen responsible for millions of deaths worldwide. Currently, the available vaccines for the prevention of S. pneumoniae infections are the 23-valent pneumococcal polysaccharide-based vaccine (PPV-23) and the pneumococcal conjugate vaccines (PCV10 and PCV13). These vaccines only cover some pneumococcal serotypes (up to 100 different serotypes have been identified) and are unable to protect against non-vaccine serotypes and non-encapsulated pneumococci. The emergence of antibiotic-resistant non-vaccine serotypes after these vaccines is an increasing threat. Therefore, there is an urgent need to develop new pneumococcal vaccines which could cover a wide range of serotypes. One of the vaccines most characterized as a prophylactic alternative to current PPV-23 or PCVs is a vaccine based on pneumococcal protein antigens. The choline-binding proteins (CBP) are found in all pneumococcal strains, giving them the characteristic to be potential vaccine candidates as they may protect against different serotypes. In this review, we have focused the attention on different CBPs as vaccine candidates because they are involved in the pathogenesis process, confirming their immunogenicity and protection against pneumococcal infection. The review summarizes the major contribution of these proteins to virulence and reinforces the fact that antibodies elicited against many of them may block or interfere with their role in the infection process.

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“…Some non-encapsulated pneumococcal lineages express a surface protein, PspK, which is important in bacterial adherence to host epithelia as well as formation of biofilms and persistence in vivo. [16][17][18][19][20] PspK was also shown to increase virulence in encapsulated strains as well [21]. Non-encapsulated pneumococci have also been shown to persist within polymicrobial biofilms and cause OM in conjunction with NTHi [22].…”

Section: Streptococcus Pneumoniaementioning

confidence: 99%

Panel 7 – Pathogenesis of otitis media – a review of the literature between 2015 and 2019

Thornton

Håkansson

Hood

et al. 2020

International Journal of Pediatric Otorhinolaryngology

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Pulmonary Disease Associated With Nonencapsulated Streptococcus pneumoniae

Cited by 11 publications

References 15 publications

Identification and molecular epidemiology of routinely determined Streptococcus pneumoniae with negative Quellung reaction results

Identification and molecular epidemiology of routinely determined Streptococcus pneumoniae with negative Quellung reaction results

Pneumococcal Choline-Binding Proteins Involved in Virulence as Vaccine Candidates

Panel 7 – Pathogenesis of otitis media – a review of the literature between 2015 and 2019

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