Pulp cell cultures obtained with two different methods for in vitro cytotoxicity tests

Cortés, Olga; García, Carmelo Conesa; Pérez, Laura; Boj, J R; Alcaina, Antonia

doi:10.1007/bf03320822

Cited by 11 publications

(9 citation statements)

References 14 publications

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“…Caries‐free, intact wisdom teeth were obtained from Birmingham Dental Hospital following ethical approval from BBC CLRN RM&G Consortium Office (Approval number: BCHCDent334.1533.TB). Human dental pulp cells (DPCs) were isolated via the explant procedure by removing the pulpal tissue, dissecting and homogenizing the tissue into small pieces and transferring into 25 cm 2 culture flasks which were supplemented with 2 ml of α‐MEM (Biosera, UK)/20% fetal calf serum (FCS), 1% L‐Glutamine and Amphotericin (1 µL/ml). Cultures were incubated at 37 °C in a humidified atmosphere containing 5% CO 2 (Panasonic/MCO‐18AC‐PE, UK).…”

Section: Methodsmentioning

confidence: 99%

Development and application of LED arrays for use in phototherapy research

Cooper

Milward

Gorecki

et al. 2017

Journal of Biophotonics

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Lasers/LEDs demonstrate therapeutic effects for a range of biomedical applications. However, a consensus on effective light irradiation parameters and efficient and reliable measurement techniques remain limited. The objective here is to develop, characterise and demonstrate the application of LED arrays in order to progress and improve the effectiveness and accuracy of in vitro photobiomodulation studies. 96-well plate format LED arrays (400-850 nm) were developed and characterised to accurately assess irradiance delivery to cell cultures. Human dental pulp cells (DPCs) were irradiated (3.5-142 mW/cm : 15-120 s) and the biological responses were assessed using MTT assays. Array calibration was confirmed using a range of optical and analytical techniques. Multivariate analysis of variance revealed biological responses were dependent on wavelength, exposure time and the post-exposure assay time (P < 0.05). Increased MTT asbsorbance was measured 24 h post-irradiation for 30 s exposures of 3.5 mW/cm at 470, 527, 631, 655, 680, 777, 798 and 826 nm with distinct peaks at 631 nm and 798 nm (P < 0.05). Similar wavelengths were also effective at higher irradiances (48-142 mW/cm ). LED arrays and high throughput assays provide a robust and reliable platform to rapidly identify irradiation parameters which is both time- and cost-effective. These arrrays are applicable in photobiomodulation, photodynamic therapy and other photobiomedical research.

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Section: Methodsmentioning

confidence: 99%

Development and application of LED arrays for use in phototherapy research

Cooper

Milward

Gorecki

et al. 2017

Journal of Biophotonics

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“…The biocompatibility of MTA has been previously investigated in vitro using a number of the cell lines: MG 63 cells, Saos2 cells, human osteoblasts and L929 fibroblasts [19,21,30,31,]. However, the use of primary cultures (in this case pulp fibroblasts) is often desirable, since they may offer increased specific sensitivity to the test agent or substance [32,33].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of Regular and White MTA and A Grey Portland Cement in Human Dental Pulp Cells

O¹,

Quesada²,

A³

2018

ojdom

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Currently many materials have been used with pulpotomies in primary teeth. These include MTA, that as a direct pulp capping material seemed to stimulate natural dentin repair at pulpal exposure sites. The composition of this product is based on Portland cement. Later, white MTA was introduced as an esthetic improvement over the original material (grey MTA). A number of studies have been performed on this new material to determine if it shows the same properties as gray MTA. The biocompatibility of MTA has been previously investigated in vitro using a number of the cell lines. However, the use of primary cultures (in this case pulp fibroblasts) is often desirable. Objective: The objective of this in vitro study was to compare the cytotoxicity in a pulp fibroblasts culture, of regular (grey) MTA, white MTA and Portland cement Material and method: A cytotoxicity study was made in a primary pulp fibroblasts culture of MTA, white and grey, and a grey Portland cement. Results: Without taking into account concentrations, there were no significant differences between regular MTA and white MTA (p=0.146). A significant difference was recorded between grey Portland cement, which were less biocompatible, and regular MTA (p=0.029), and white MTA (p=0.002). Conclusion: The results found in the present study might be an additional argument to support that MTA is biocompatible, and may be considered as a pulp dressing agent in pulpotomized primary molars with carious pulp exposures. However, the grey Portland cement, under the same conditions, shows reduced biocompatibility.

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“…The implant site was the fresh extraction socket of adult rat, mandibular M1 teeth. Enzymatic digestion conditions for 4-dpn rat molar tooth bud dissociation were defined, guided by prior published reports (Kikuchi et al , 1994; Cortes et al , 2006). Identical experimental and control scaffolds were implanted either in the jaw or in the omentum of adult rat hosts and allowed to grow for 12 wks.…”

Section: Discussionmentioning

confidence: 99%

“…Approaches to mesenchymal DSC enrichment include in vitro differentiation models (Kikuchi et al , 1994; Cortes et al , 2006) and analyses of DSC progenitors in transgenic mice (Braut et al , 2003; Mina and Braut, 2004). Characterization of epithelial DSCs has been more difficult, largely due to the fact that epithelial DSCs are no longer present in erupted teeth, and has focused on rodent models exhibiting continuously growing incisor and/or molar tooth eruption (Kikuchi et al , 1994; Tummers and Thesleff, 2003; Harada and Ohshima, 2004; Kawano et al , 2004).…”

Section: Introductionmentioning

confidence: 99%

Bioengineered Dental Tissues Grown in the Rat Jaw

et al. 2008

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Our long-term objective is to develop methods to form, in the jaw, bioengineered replacement teeth that exhibit physical properties and functions similar to those of natural teeth. Our results show that cultured rat tooth bud cells, seeded onto biodegradable scaffolds, implanted into the jaws of adult rat hosts and grown for 12 weeks, formed small, organized, bioengineered tooth crowns, containing dentin, enamel, pulp, and periodontal ligament tissues, similar to identical cellseeded scaffolds implanted and grown in the omentum. Radiographic, histological, and immunohistochemical analyses showed that bioengineered teeth consisted of organized dentin, enamel, and pulp tissues. This study advances practical applications for dental tissue engineering by demonstrating that bioengineered tooth tissues can be regenerated at the site of previously lost teeth, and supports the use of tissue engineering strategies in humans, to regenerate previously lost and/or missing teeth. The results presented in this report support the feasibility of bioengineered replacement tooth formation in the jaw.

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Pulp cell cultures obtained with two different methods for in vitro cytotoxicity tests

Cited by 11 publications

References 14 publications

Development and application of LED arrays for use in phototherapy research

Development and application of LED arrays for use in phototherapy research

Biocompatibility of Regular and White MTA and A Grey Portland Cement in Human Dental Pulp Cells

Bioengineered Dental Tissues Grown in the Rat Jaw

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