Pulp Extracellular Matrix

Veis, Arthur; Goldberg, Michel

doi:10.1007/978-3-642-55160-4_3

Cited by 6 publications

(9 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, the characteristic of specific ECM and its modulations have been extensively investigated in order to control cell behaviors toward disease attenuation or healing repair. The ECM of dental pulp are rich with hyaluronan, glycosaminoglycans, and proteoglycans that are all kept together by a network of thin collagen fibrils, reticular fibrils, and fibronectin (Veis and Goldberg 2014). Due to major differences in the pulp ECM, pulp is often a non-mineralized tissue, whereas dentin is a non-collagenous ECM component.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Matrix Derived From Dental Pulp Stem Cells Promotes Mineralization

Nowwarote

Petit

Ferre

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Background: Extracellular matrix (ECM) plays a pivotal role in many physiological processes. ECM macromolecules and associated factors differ according to tissues, impact cell differentiation, and tissue homeostasis. Dental pulp ECM may differ from other oral tissues and impact mineralization. Thus, the present study aimed to identify the matrisome of ECM proteins derived from human dental pulp stem cells (DPSCs) and its ability to regulate mineralization even in cells which do not respond to assaults by mineralization, the human gingival fibroblasts (GF).Methods: ECM were extracted from DPSCs cultured in normal growth medium supplemented with L-ascorbic acid (N-ECM) or in osteogenic induction medium (OM-ECM). ECM decellularization (dECM) was performed using 0.5% triton X-100 in 20 mM ammonium hydroxide after 21 days. Mass spectrometry and proteomic analysis identified and quantified matrisome proteins.Results: The dECM contained ECM proteins but lacked cellular components and mineralization. Interestingly, collagens (COL6A1, COL6A2, and COL6A3) and elastic fibers (FBN1, FBLN2, FN1, and HSPG2) were significantly represented in N-ECM, while annexins (ANXA1, ANXA4, ANXA5, ANXA6, ANXA7, and ANXA11) were significantly overdetected in OM-ECM. GF were reseeded on N-dECM and OM-dECM and cultured in normal or osteogenic medium. GF were able to attach and proliferate on N-dECM and OM-dECM. Both dECM enhanced mineralization of GF at day 14 compared to tissue culture plate (TCP). In addition, OM-dECM promoted higher mineralization of GF than N-dECM although cultured in growth medium.Conclusions: ECM derived from DPSCs proved to be osteoinductive, and this knowledge supported cell-derived ECM can be further utilized for tissue engineering of mineralized tissues.

show abstract

Section: Introductionmentioning

confidence: 99%

Extracellular Matrix Derived From Dental Pulp Stem Cells Promotes Mineralization

Nowwarote

Petit

Ferre

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…pECM is a loose highly hydrated viscous extracellular connective tissue matrix of collagenous and non-collagenous proteins, rich in hyaluronan, glycosaminoglycans and proteoglycans, all held together in a network of thin collagen fibrils, reticular fibrils and fibronectin. Col I and III are the two major structural components of the pECM [ 102 ]. The non-collagenous component contains a significant number of well-characterized bioactive regulatory molecules, such as DSP, DPP, BSP, DMP-1, OP, MEPE and members of SIBLINGs [ 103 ], in addition to other various non-phosphorylated proteins, various GFs and their receptors, and a variety of enzymes [ 102 ].…”

Section: Hydrogels Used In Dentin–pulp Complex Regenerationmentioning

confidence: 99%

“…Col I and III are the two major structural components of the pECM [ 102 ]. The non-collagenous component contains a significant number of well-characterized bioactive regulatory molecules, such as DSP, DPP, BSP, DMP-1, OP, MEPE and members of SIBLINGs [ 103 ], in addition to other various non-phosphorylated proteins, various GFs and their receptors, and a variety of enzymes [ 102 ]. Cells grown on bovine pECM-coated cultureware did not demonstrate enhanced proliferation as compared to cells grown on uncoated control.…”

Section: Hydrogels Used In Dentin–pulp Complex Regenerationmentioning

confidence: 99%

Hydrogels and Dentin–Pulp Complex Regeneration: From the Benchtop to Clinical Translation

et al. 2020

View full text Add to dashboard Cite

Dentin–pulp complex is a term which refers to the dental pulp (DP) surrounded by dentin along its peripheries. Dentin and dental pulp are highly specialized tissues, which can be affected by various insults, primarily by dental caries. Regeneration of the dentin–pulp complex is of paramount importance to regain tooth vitality. The regenerative endodontic procedure (REP) is a relatively current approach, which aims to regenerate the dentin–pulp complex through stimulating the differentiation of resident or transplanted stem/progenitor cells. Hydrogel-based scaffolds are a unique category of three dimensional polymeric networks with high water content. They are hydrophilic, biocompatible, with tunable degradation patterns and mechanical properties, in addition to the ability to be loaded with various bioactive molecules. Furthermore, hydrogels have a considerable degree of flexibility and elasticity, mimicking the cell extracellular matrix (ECM), particularly that of the DP. The current review presents how for dentin–pulp complex regeneration, the application of injectable hydrogels combined with stem/progenitor cells could represent a promising approach. According to the source of the polymeric chain forming the hydrogel, they can be classified into natural, synthetic or hybrid hydrogels, combining natural and synthetic ones. Natural polymers are bioactive, highly biocompatible, and biodegradable by naturally occurring enzymes or via hydrolysis. On the other hand, synthetic polymers offer tunable mechanical properties, thermostability and durability as compared to natural hydrogels. Hybrid hydrogels combine the benefits of synthetic and natural polymers. Hydrogels can be biofunctionalized with cell-binding sequences as arginine–glycine–aspartic acid (RGD), can be used for local delivery of bioactive molecules and cellularized with stem cells for dentin–pulp regeneration. Formulating a hydrogel scaffold material fulfilling the required criteria in regenerative endodontics is still an area of active research, which shows promising potential for replacing conventional endodontic treatments in the near future.

show abstract

“…The presence of enzymes specifically associated to pulp cell functions has also been established. 12 Dentinal tubules are invaded by oral bacteria. This is followed by the colonization and a subsequent infection of the root canal system.…”

Section: The Healthy Dental Pulp: the Cellsmentioning

confidence: 99%

“…Whereas collagens type III, V, VI have been also identified in the pulp, the dentin collagen is almost exclusively of the type I. 12 Antigen-presenting cells (APC) : The Major Histocompatibility Complex (MHC) region encodes transplantation class I and class II molecules. Dendritic cells, macrophages, B-lymphocytes, endothelial cells, and various epithelial and mesenchymal cells are constitutive or inducible cells expressing Class II MHC molecules.…”

Section: Degradation Of the Dental Pulp Due To Degradation By Endogenmentioning

confidence: 99%

The healthy root pulp, degradation and regeneration

Goldberg¹

2019

JDHODT

Self Cite

View full text Add to dashboard Cite

show abstract

Pulp Extracellular Matrix

Cited by 6 publications

References 39 publications

Extracellular Matrix Derived From Dental Pulp Stem Cells Promotes Mineralization

Extracellular Matrix Derived From Dental Pulp Stem Cells Promotes Mineralization

Hydrogels and Dentin–Pulp Complex Regeneration: From the Benchtop to Clinical Translation

The healthy root pulp, degradation and regeneration

Contact Info

Product

Resources

About