Pulp lignification in Korla fragrant pear

Mamat, Aisajan; Ayup, Mubarek; Zhang, Xiaoli; Ma, Kai; Mei, Chuang; Pu, Yan; Huang, Liqun; Wang, Jixun

doi:10.17660/ejhs.2019/84.5.2

Cited by 10 publications

(5 citation statements)

References 45 publications

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“…However, lignin content continuously decreased with fruit maturation (Fig. 3), as reported by Mamat et al (2019) and Zhang et al (2021),…”

Section: • -supporting

confidence: 77%

ROS-mediated Lignin Biosynthesis is Associated with Cell Wall Expansion in Asian Pear (Pyrus pyrifolia Nakai) During Fruit Development and Ripening

Lee,

Baek,

et al. 2022

HST

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Fruit growth and development is regulated by cell expansion, which is a culmination of balanced cell stiffening and loosening. Reactive oxygen species (ROS) are crucial factors regulating cell expansion. In the present study, we investigated how cell wall expansion is regulated by ROS homeostasis in two Asian pear (Pyrus pyrifolia Nakai) cultivars with different maturity periods. ROS production, lignin contents, peroxidase activity, and gene expression levels were measured during pear fruit development and ripening. An increase in fruit fresh weight was observed 72 and 87 days after full bloom (DAFB) in early-maturing cultivar 'Wonhwang' and late-maturing cultivar 'Niitaka', respectively; the former exhibited higher weights than the latter until 132 DAFB. In both cultivars, ROS (O 2•and H 2 O 2 ) content gradually decreased up to 132 DAFB; higher levels were detected in 'Niitaka' than in 'Wonhwang', which was consistent with the superoxide dismutase (SOD) activity. H 2 O 2 -induced lignin content decreased after maintaining a high level up to 87 DAFB in 'Niitaka', whereas it continued to decrease during fruit development and ripening after 57 DAFB in 'Wonhwang'. In 'Niitaka', activities of lignin biosynthesis-related enzymes, guaiacol peroxidase (GPOX) and coniferyl alcohol peroxidase (CPOX), rapidly decreased from 102 to 132 DAFB, but remained higher than those in 'Wonhwang'; this result was consistent with the expression patterns of peroxidase genes (PpPOX1 and PpPOX4). The expression level of the cell wall loosening-related gene, expansin 1 (PpEXP1), was elevated only in the middle stage of fruit development and ripening, while the expression level of xyloglucan endotransglucosylases 1 (PpXET1) rapidly increased after 57 and 87 DAFB in 'Wonhwang' and 'Niitaka', respectively. These results indicate that ROS-mediated lignin synthesis through peroxidase is associated with cell wall expansion in Pyrus pyrifolia.

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“…However, lignin content continuously decreased with fruit maturation (Fig. 3), as reported by Mamat et al (2019) and Zhang et al (2021),…”

Section: • -supporting

confidence: 77%

ROS-mediated Lignin Biosynthesis is Associated with Cell Wall Expansion in Asian Pear (Pyrus pyrifolia Nakai) During Fruit Development and Ripening

Lee,

Baek,

et al. 2022

HST

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“…Lignin composition varies among these cell types, in TEs, mainly composed of G-units, and the sclerenchyma fibres have lignified secondary cell walls mainly composed of S-units [25][26] . In our previous research, we found that sinapyl alcohol, which gives rise to S-unit, was the dominant lignin monomer in Korla pear pulp, accounting for 77.2% of total alcohol monomer [21] . These observations have led to the idea that stone cell differentiation in pear pulp might proceed through in a cell-autonomous process.…”

Section: Discussionmentioning

confidence: 89%

“…Stone cells are a type of sclerenchyma cell formed by the secondary thickening of cell walls followed by the deposition of lignin on the primary walls of parenchyma cells [19][20][21] . Our results showed that stone cell formation mainly occurred during the early stage of fruit development.…”

Section: Discussionmentioning

confidence: 99%

“…However, the expression levels of genes positively regulated with lignin content were universally much higher than that of negatively regulated genes, indicating that they are the core genes responsible for lignin synthesis during stone cell formation in pear fruits. Genes and enzymes involved in monolignol biosynthesis have been studied extensively in different pear varieties [1,2,8,21,[60][61] . Therefore roles of these genes during lignin accumulation in pear pulp will not be discussed in detail.…”

Section: Discussionmentioning

confidence: 99%

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Integrated transcriptomic and proteomic analysis reveal new insights into stone cell formation in Korla pear

Mamat¹,

Zhang²,

Xu³

et al. 2020

Preprint

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Background Korla fragrant pear(P•sinkiangensis Yü)is a famous local variety of Xinjiang China. One difficulty is the high stone cell content of these pears, which causes the formation of rough skins on the fruit. To elucidate the underlying mechanisms of stone cell formation, parallel analyses of the transcriptome and proteome was performed to identify important regulators and pathways involved in stone cell formation.Results Fruit samples were collected at three important time points depending on the stages of stone cell formation (20, 50 and 80 days after flowering). A total of 24268 differentially expressed genes (DEGs) and 1011 differentially accumulated proteins (DAPs) were identified from all the time points. Function analysis of the differential genes/proteins revealed that a set of candidates was associated with stone cell formation. These candidates mainly enriched in pathways involved in lignin biosynthesis, cellulose and xylan biosynthesis, S-adenosylmethionine (SAM) metabolic process, Reactive oxygen species (ROS) production, and cell death. We mined a total of 253 DEGs, and 100 DAPs, 63 of which were significantly changed at both the transcript and protein levels during fruit development.Conclusions Our findings reveal that some intriguing genes/proteins were previously unrecognized related with the sclereid formation, which provided new insights into molecular processes regulating sclereid accumulation in pear pulp.

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“…Six PAL genes were recently identified, characterised and expressed in M. truncatula; these genes were also differentially expressed in different tissues and in response to different abiotic stresses (Ren et al 2019). Similarly, three Pyrus bretschneideri PAL genes were induced in response to abiotic stress to varying degrees (Mamat et al 2019). Together, these studies indicate that the general function of PAL genes is clear; however, the functional differences of individual PAL genes are not well understood in many species.…”

Section: Introductionmentioning

confidence: 93%

Molecular characterisation of

Feng

Huang

Zhang

et al. 2022

Crop & Pasture Science

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Phenylalanine ammonia lyase (PAL) is the first enzyme in the phenylpropanoid pathway and plays a critical role in plant growth, development and stress defence. However, there have been few reports of the PAL gene family in lucerne (also known as alfalfa, Medicago sativa L.), one of the most important forage legume species worldwide. In this study, we report that PAL in lucerne is encoded by a family of seven genes: MsPAL1–MsPAL7. Furthermore, a comprehensive genome-wide bioinformatics analysis of the MsPAL gene family is presented, including chromosomal locations, phylogenetic relationships, gene structures and conserved motifs. The cis-elements and potential biological functions of these genes were investigated, revealing the potential roles of MsPAL members in response to various stresses. RT-qPCR results showed that the expression of MsPAL6 was significantly upregulated under both salinity- and waterlogging-stress conditions. Other MsPAL members such as MsPAL1 and MsPAL2 were downregulated under saline conditions and upregulated significantly after waterlogging stress. Our findings provide useful information for further practical analyses and for the genetic improvement of abiotic stress tolerance of lucerne.

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Pulp lignification in Korla fragrant pear

Cited by 10 publications

References 45 publications

ROS-mediated Lignin Biosynthesis is Associated with Cell Wall Expansion in Asian Pear (Pyrus pyrifolia Nakai) During Fruit Development and Ripening

ROS-mediated Lignin Biosynthesis is Associated with Cell Wall Expansion in Asian Pear (Pyrus pyrifolia Nakai) During Fruit Development and Ripening

Integrated transcriptomic and proteomic analysis reveal new insights into stone cell formation in Korla pear

Molecular characterisation of

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