The paper concerns a heat transfer characteristics for a pulsating heat pipe (PHP) under hyper-gravity condition. Two loops of pulsating heat pipe with the internal diameter of 1.5 mm were filled and tested with HFE-7000 as a working fluid. The experimental setup allows for the investigation of two crucial parameters affecting the thermal process i.e. rotational speed (acceleration from 1 to 8g) and filling ratio (44 and 66, %). As a result, the dependences of the thermal resistance on the rotational speeds were obtained, thus declaring the optimal conditions for the heat transport process. The increase in filing ratio (FR) of the working fluid in hyper-gravity condition improves the thermal efficiency of PHP, as well as the direction of the evaporation section bending.