Pulsed Electric Field for protein release of the microalgae Chlorella vulgaris and Neochloris oleoabundans

Lam, G.P. ‘t; Postma, P.R.; Fernandes, Diana A.; Timmermans, R.A.H.; Vermuë, M.H.; Barbosa, María J.; Eppink, M.H.M.; Wijffels, René H.; Olivieri, Giuseppe

doi:10.1016/j.algal.2017.03.024

Cited by 113 publications

(34 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The protein yields obtained with the wild type (cc-124) after PEF treatment are similar to those found for other microalgae with a rigid outer cell wall, like Chlorella vulgaris , Neochloris oleoabundans , and Auxenochlorella protothecoides . 6−8 The study of ‘t Lam et al 7 even showed that PEF allowed at least a 4-fold lower protein release than bead milling. It is therefore reasonable that in general, protein release by means of PEF is limited by the presence of an outer cell wall.…”

Section: Resultsmentioning

confidence: 99%

“…The conditions needed for PEF treatment were tested in a batch electroporation apparatus (Gene-Pulser XcellTM Bio-Rad, USA), which was described in ‘t Lam et al: 7 …”

Section: Methodsmentioning

confidence: 99%

“…Dry weight analysis was determined according to the work of Lamers et al 15 According to ‘t Lam et al, 7 the conductivity of the samples was determined.…”

Section: Methodsmentioning

confidence: 99%

“…Modified Lowry protein assays (Thermo-scientific) were used to analyze the water-soluble protein content in the samples taken according to our previous work: 7 After colorimetric reaction, absorbance was measured spectrophotometrically at a wavelength of 750 nm. The total protein content was determined using total nitrogen analysis (“Kjehldahl”) and a conversion factor for C. reinhardtii of 4.58.…”

Section: Methodsmentioning

confidence: 99%

“…The applied energy input was calculated using the work of ‘t Lam et al: 7 In this equation, E = the electric field applied (V/m), t p is the pulse length (s), and N is the number of pulses applied. The conductivity “σ” was measured prior to the PEF treatment (S/m), and the biomass concentration ( C x ) was provided in kilograms dry weight per cubic meter.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Mild and Selective Protein Release of Cell Wall Deficient Microalgae with Pulsed Electric Field

Lam

Kolk

Chordia

et al. 2017

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Pulsed electric field (PEF) is considered to be a very promising technology for mild cell disruption. The application of PEF for microalgae that have a rigid cell wall, however, is hampered by the presence of that rigid outer cell wall. A cell wall free mutant of C. reinhardtii was used to mimic pretreated microalgae with removed cell wall, to investigate the possibility of using PEF for protein release from microalgae. A complete release of hydrophilic proteins from the cell wall free mutants was observed whereas PEF treatment on the cell wall containing species resulted in substantially lower protein yields. Additional experiments showed that even at low energy input (0.05 kWh/kgbiomass), still about 70% of the proteins could be released with respect to bead beating as reference. These released proteins were water-soluble while the hydrophobic chlorophyll remained mainly entrapped in cell particles. SEM-analysis of these cell particles showed that PEF only opened the cells, instead of completely fragmenting them into smaller particles. These results indicate that PEF is an energy-efficient cell disruption method for selective release of water-soluble proteins, after the microalgal outer cell wall is removed. Enzymatic pretreatment to degrade the cell walls before PEF treatment was shown to be an efficient method to remove the cell wall.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The conditions needed for PEF treatment were tested in a batch electroporation apparatus (Gene-Pulser XcellTM Bio-Rad, USA), which was described in ‘t Lam et al: 7 …”

Section: Methodsmentioning

confidence: 99%

“…Dry weight analysis was determined according to the work of Lamers et al 15 According to ‘t Lam et al, 7 the conductivity of the samples was determined.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations