2023
DOI: 10.3390/pathogens12070966
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Pulsed-Field Gel Electrophoresis Analysis of Bovine Associated Staphylococcus aureus: A Review

Abstract: For decades now, DNA fingerprinting by means of pulsed-field gel electrophoresis (PFGE) continues to be the most widely used to separate large DNA molecules and distinguish between different strains in alternating pulses. This is done by isolating intact chromosomal DNA and using restriction enzymes with specific restriction sites to generate less than 30 restriction fragments from 50 Kb to 10 Mbp. These results make clone-specific band profiles easy to compare. Specialized equipment is required for the optimi… Show more

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Cited by 4 publications
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“…Physical, chemical, cellular, and microbiological changes in mastitic milk cause economic losses worldwide and promote reduced reproductive life span of suffered animals (Joy et al 2023). Many outbreaks have occurred due to unpasteurized mastitic milk (Dendani and Arcangioli 2023). This milk led to many zoonotic diseases like Brucellosis, Mycoplasma infections, tuberculosis and leptospirosis.…”
Section: Introductionmentioning
confidence: 99%
“…Physical, chemical, cellular, and microbiological changes in mastitic milk cause economic losses worldwide and promote reduced reproductive life span of suffered animals (Joy et al 2023). Many outbreaks have occurred due to unpasteurized mastitic milk (Dendani and Arcangioli 2023). This milk led to many zoonotic diseases like Brucellosis, Mycoplasma infections, tuberculosis and leptospirosis.…”
Section: Introductionmentioning
confidence: 99%