Pulsed-Field Gel Electrophoresis and Its Application in the Physical Analysis of the Bovine Major Histocompatibility Complex

Bensaïd, Albert; Young, J. R.; Kaushal, Anju; Teale, A.J.

doi:10.1201/9781003066712-9

Cited by 3 publications

(2 citation statements)

References 1 publication

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“…The BoLA region includes approximately 154 predicted functional genes which could be categorized into three classes: class I, II and III 14) . BoLA class I covers the region on ch23 from 770 Kb to 1650 Kb, including classical class I gene BoLA-A and BoLA-B that are located 200 Kb far from each other which BoLA-A is closer to telomeric side of ch23 15) . In cattle, none of these classical class I genes identified is consistently expressed, and haplotypes differ from one to another in both the gene number and composition.…”

Section: Genomic Organization Of Bolamentioning

confidence: 99%

Association of <i>BoLA-DRB3</i> with bovine leukemia virus

Aida

2022

MHC

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The major histocompatibility complex (MHC) of cattle is known as BoLA and located on chromosome 23. It was found that BoLA shows significant differences in genome organization with human MHC but shares similar protein structures and functions. Among the entire BoLA class II subregion, BoLA-DRB3 is the most polymorphic and functional gene that has been intensively studied its correlation with various cattle infection diseases and economic traits. In particularly, BoLA-DRB3 polymorphism has been linked to resistance or susceptibility of bovine leukemia virus (BLV) infection outcome, transmission and disease progression. BLV leads to enzootic bovine leucosis which is the most common neoplastic disease in cattle and leads to severe financial loss in industry worldwide. As a result, genetic selection of BLV-resistant animals, as well as the preferential culling of BLV-susceptible animals, based on BolA-DRB3 polymorphism, serves as a promising strategy for the control of BLV spreading and disease risk estimation, especially in the time there are no vaccines and treatment available. In this review, we outline the BoLA system in protein and genetic levels and focus particularly on the literatures of association between BoLA-DRB3 polymorphism with BLV.

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Section: Genomic Organization Of Bolamentioning

confidence: 99%

Association of <i>BoLA-DRB3</i> with bovine leukemia virus

Aida

2022

MHC

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“…The MCH is divided into three groups in literature and they are namely class I, class II and class III (Behl et al, 2012). Concurrently, the bovine MHC BoLAwhich is localized to chromosome 6 of cattle genome (BTA 6) has been shown to comprise of two tightly linked loci, namely A and B (Bensaid et al, 1991). Further sub-divisions of the BoLA gene into various loci including DRA, DRB, DRQ, DQA and DQB among others were reported by Groenen et al(1990).…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms of BoLA-DRB 3.2 gene and associated genetic relationships among four strains of Tanzania shorthorn zebu cattle

2018

The Korean Society of Animal Breeding and Genomics

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Bovine Lymphocyte Antigen (BoLA) genes play important roles in resistance or susceptibility of cattle to infectious diseases. The BoLA gene comprises of several loci including the most polymorphic site namely DRB 3.2. We amplified 200 DNA samples and sequenced 270 bp comprising exon 2 of BoLA-DRB 3.2 in four strains of Tanzania shorthorn zebu (TSZ) cattle (Tarime, Sukuma, Maasai, Singida white) and one breed, namely Friesian. Sequences were processed on Finch TV, aligned on Basic Local Alignment Search Tool (BLAST) online, and matched to amino acids using MEGA 6. Frequency of each allele was computed as proportion of total alleles in each population. Chi-square was used to test significance in allele frequencies. Heterozygosities were computed using Poptree 2. Putative evolutionary relationships were evaluated by Nei genetic distances. Thirty four alleles were determined, of which nine alleles are novel. The greatest number of alleles was determined in Tarime and Singida white and the lowest in Friesian. Heterozygosities were high within the animals. Phylogenetic tree showed two major clusters one for TSZ and a second for Friesian. Polymorphism at DRB 3.2 in TSZ could be one explanation for their ability to withstand various diseases and we recommend further evaluations in the breed.

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