Pulsed Galvanostatic Control of Ionophore-Based Polymeric Ion Sensors

Shvarev, Alexey; Bakker, Eric

doi:10.1021/ac034409t

Cited by 110 publications

(167 citation statements)

References 37 publications

Supporting

Mentioning

160

Contrasting

Unclassified

Order By: Relevance

“…53 Essentially, the extraction of analyte ions from the sample is not controlled chemically by an ion-exchanger, but imposed electrochemically. For this reason, such membranes are ideally void of ion-exchanger properties.…”

Section: Beyond Potentiometry: Pulstrodesmentioning

confidence: 99%

Modern directions for potentiometric sensors

Bakker¹,

Chumbimuni‐Torres²

2008

J. Braz. Chem. Soc.

Self Cite

View full text Add to dashboard Cite

Este artigo apresenta uma revisão dos mais recentes progressos dos eletrodos íons-seletivos de membrana polimérica. É discutida uma essência curta da teoria básica, enfatizando como a força eletromotriz pode ser usada para calcular as constantes de ligação do ionóforo, e como a seletividade e o limite de detecção estão relacionados aos processos de membrana mais importantes. Os recentes progressos na diminuição dos limites de detecção dos ISEs são descritos, incluindo recentes tentativas no desenvolvimento de ISEs de contato sólido, e inovações na detecção de quantidades ultra-pequenas de íons em baixas concentrações. Esses progressos têm alçado o caminho para usar sensores potenciométricos em bioanálise com afinidade ultra-sensível junto com nanopartículas usadas como marcadores. Os resultados recentes estabelecem que a potenciometria compara-se favoravelmente com a análise de redissolução eletroquímica. Outros novos progressos com os eletrodos de íon-seletivo são também descritos, incluindo o conceito de potenciometria de calibração interna, coulometria de corrente controlada, cronopotenciometria pulsada, e titulação rápida localizada com membranas íon-seletivas para desenhar sensores de detecção direta da acidez total sem perturbação da amostra. Estes progressos têm aberto um amplo campo para novos desenvolvimentos e aplicações nesta área. This paper gives an overview of the newest developments of polymeric membrane ion-selective electrodes. A short essence of the underlying theory is given, emphasizing how the electromotive force may be used to assess binding constants of the ionophore, and how the selectivity and detection limit are related to the basic membrane processes. The recent developments in lowering the detection limits of ISEs are described, including recent approaches of developing all solid state ISEs, and breakthroughs in detecting ultra-small quantities of ions at low concentrations. These developments have paved the way to use potentiometric sensors as in ultra-sensitive affinity bioanalysis in conjunction with nanoparticle labels. Recent results establish that potentiometry compares favorably to electrochemical stripping analysis. Other new developments with ion-selective electrodes are also described, including the concept of backside calibration potentiometry, controlled current coulometry, pulsed chronopotentiometry, and localized flash titration with ion-selective membranes to design sensors for the direct detection of total acidity without net sample perturbation. These developments have further opened the field for exciting new possibilities and applications.

show abstract

Section: Beyond Potentiometry: Pulstrodesmentioning

confidence: 99%

Modern directions for potentiometric sensors

Bakker¹,

Chumbimuni‐Torres²

2008

J. Braz. Chem. Soc.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Alternatively, a chemical regeneration of the membrane is possible [11], which seems most attractive via sample pH changes as demonstrated with chemically modified membrane compositions. [12] Recently, a pulsed chrono-potentiometric control of similarly configured membrane electrodes, so-called pulstrodes, has afforded an instrumental control over the ion extraction process [13][14][15][16]. Because of a potentiostatic stripping pulse applied after a current-controlled ion extraction pulse, the sensing membrane is regenerated after each pulse cycle.…”

Section: Introductionmentioning

confidence: 99%

Reversible detection of proteases and their inhibitors by a pulsed chronopotentiometric polyion-sensitive electrode

Xu¹,

Shvarev

Makarychev-Mikhailov³

et al. 2008

Analytical Biochemistry

Self Cite

View full text Add to dashboard Cite

Polymer membrane electrodes operated by pulsed chronopotentiometry have recently been introduced to replace traditional ion-selective electrodes for a number of applications. While ionselective electrodes for the polycation protamine have been reported, for instance, a pulsed chronopotentiometric readout mode (called here pulstrode) provides improved stability and reproducibility while exhibiting sufficient selectivity for the direct detection of protamine in undiluted whole blood samples. Here, such protamine sensitive pulstrodes are applied for the real time detection of the activity of the protease trypsin and its soybean inhibitor. This is possible because small fragments produced by the trypsin digestion are not detectable by the protamine sensing membrane. The real time response to the proteolytic reaction is shown to exhibit good reproducibility and reversibility, and the initial reaction rate is dependent on the concentration of the protease and its inhibitor.

show abstract

“…Typically, these electrodes have been extensively investigated for biosensing via zero-current potentiometry. Alternatively, ion-selective membranes can be interrogated under galvanostatic control and used for the chronopotentiometric detection in analogy to zero-current potentiometry [27,28]. The discovery of pulsed galvanostatic ion sensors, which work under periodic galvanostatic polarization, can provide a rapid, reproducible and continuous potentiometric sensing format with improved upper detection limit, sensitivity, selectivity and reversibility [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

Ding

Wang

et al. 2016

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

a b s t r a c tWe report here on the development of a chronopotentiometric assay for microcystin-LR based on enzymatic inhibition. The inhibition of protein phosphatase by microcystin-LR can be sensed potentiometrically by using 4-nitrophenyl phosphate as an enzyme substrate. A solid-contact ion-selective electrode (ISE) with poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) as a transduction layer has been designed for potentiometric biosensing using the pulsed galvanastatic technique. By applying an anodic current, the enzymatic generated p-nitrophenol can be extracted into the polymeric membrane with tetradodecylammonium tetrakis(4-chlorophenyl)-borate to produce the chronopotentiometric signal. Meanwhile, a controlled voltage was applied to refresh the membrane for multiple consecutive measurements. The proposed potentiometric assay showed a linear response for microcystin-LR in the range 1-100 g/L with a detection limit of 0.5 g/L (3 ). We believe that the proposed method can be employed for sensitive, rapid and reliable determination of analytes involved in enzyme inhibition.

show abstract

Pulsed Galvanostatic Control of Ionophore-Based Polymeric Ion Sensors

Cited by 110 publications

References 37 publications

Modern directions for potentiometric sensors

Modern directions for potentiometric sensors

Reversible detection of proteases and their inhibitors by a pulsed chronopotentiometric polyion-sensitive electrode

Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

Contact Info

Product

Resources

About