Pumpkin Seed Oil-Loaded Niosomes for Topical Application: 5α-Reductase Inhibitory, Anti-Inflammatory, and In Vivo Anti-Hair Loss Effects

Abstract

Pumpkin seed oil (PSO)-loaded niosomes were prepared from Tween 20 and cholesterol by ethanol injection. Confocal microscopy showed better skin permeation and hair follicle accumulation of the niosomes compared to the PSO solution. The PSO-loaded niosomes inhibited 5α-reductase activity in DU-145 cells and hindered IL-6 activity in RAW 264.7 cells. These effects indicated the great potential of PSO-loaded niosomes to reduce hair loss. The hair scalp serum with PSO-loaded niosomes did not show irritation to reconstructed human skin. This formulation presented a significant decrease in the percentage of fallen hairs by 44.42% in the in vivo 60-second hair count experiment and a significant increase in the anagen to telogen (A/T) ratio (1.4-fold) in the TrichoScan® evaluation after 8 weeks of treatment compared to the initial conditions, indicating the promising efficacy of PSO-loaded niosomes as a natural alternative for anti-hair loss therapy.

Cannabidiol-Loaded Nanostructured Lipid Carriers (NLCs) for Dermal Delivery: Enhancement of Photostability, Cell Viability, and Anti-Inflammatory Activity

“…CBD extract and CBD-NLCs with concentrations showing cell viabilities of higher than 80% were incubated with RAW264.7 cell macrophages as previously described by Teeranachaideekul et al [ 29 ] with some modifications. The cells were plated in a 24-well plate at a concentration of 1 × 10 5 cells/well and incubated at 37 °C under 5% CO 2 for 24 h. After that, the culture medium was removed and replaced with fresh culture medium containing test samples for 2 h. Then, 1 µg/mL lipopolysaccharide (LPS) was added to each well for 24 h of incubation at 37 °C under 5% CO 2 .…”

“…RAW264.7 cell macrophages treated with and without LPS were used as positive and negative controls, respectively. Diclofenac sodium at the concentration of 1000 μM was used as a standard anti-inflammatory agent [ 29 ]. The concentration of IL-6 in culture media was determined using ELISA MAX TM Standard Set Mouse IL-6 (Biolegend, California, USA) according to the manufacturer’s instructions in a 96-well plate.…”

Regulatory Effects of Thai Rice By-Product Extracts from Oryza sativa L. cv. Bue Bang 3 CMU and Bue Bang 4 CMU on Melanin Production, Nitric Oxide Secretion, and Steroid 5α-Reductase Inhibition

“…The modulation of the SRD5A gene expression was observed in prostate tumor cells as previously described [ 10 ]. The DU-145 cells were used as an in vitro cell model to determine the expression of 5α-reductase for anti-hair loss applications [ 100 , 101 , 102 ] because the action of 5α-reductase was obviously evaluated in prostate cells [ 103 ]. Total RNA was isolated from the DU-145 cells treated with samples (RBO, 0.25 mg/mL; DFRB, 0.50 mg/mL; and H, 0.10 mg/mL) or SRD5A enzyme inhibitors (dutasteride and finasteride at a concentration of 0.10 mg/mL) for 24 h. The RNA extraction process was performed using the E.Z.N.A.…”

Nutritional, bioactive components and health properties of the milpa triad system seeds (corn, common bean and pumpkin)

“…These results suggest that the intake of PUFA in these seeds favors the improvement of the oxidant state and a hypotriglyceridemic effect ( 155 ). A study showed that pumpkin seed oil-loaded niosomes displayed a decrement in in vivo hair loss of 44.42%, representing 1.4-fold higher than commercial treatments ( 156 ). Authors also evaluated the cellular antiinflammatory activity of the experimental material, finding that functionalized niosomes inhibited 5α-reductase activity and hindered IL-6 activity in DU-145 and RAW 264.7 cellular models, respectively ( 156 ).…”