The mismatch between physical brightness and perceived brightness of display devices in different light environments is increasingly serious. This paper analyzes the perception mechanism of display brightness according to the structure and perception characteristics of human visual system. Different display stimuli were further designed for visual perception experiments, and the influence of relevant objective quantities on the evaluation of perceived brightness was studied, so as to establish a display brightness perception model based on retinal imaging. Ultimately, the perceived brightness of the display can be characterized by the display of physical luminance and pupil diameter, and it has a high correlation with the perceived experimental values.