Purification and analysis of DNases of Tritrichomonas foetus: Evidence that these enzymes are glycoproteins

Greenwell, Pamela; Younes, Maha; Rughooputh, Sanjiv

doi:10.1016/j.ijpara.2007.10.012

Cited by 9 publications

(7 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For Tritrichomonas foetus, a protozoan that parasitizes cows, DNase plays a role in pathogenicity and adhesion (Greenwell et al 2008). According to our results, the M. anisopliae DNase is important in the infection of R. microplus and D. peruvianus, but probably not in the early stages of the infection process.…”

Section: Discussionmentioning

confidence: 63%

Metarhizium anisopliae host–pathogen interaction: differential immunoproteomics reveals proteins involved in the infection process of arthropods

et al. 2010

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 63%

Metarhizium anisopliae host–pathogen interaction: differential immunoproteomics reveals proteins involved in the infection process of arthropods

et al. 2010

View full text Add to dashboard Cite

“…Here, we applied ELLA, a lectin ELISA, to characterize the glycosylation pattern of TLA. ELLA is commonly used for analysis of glycoconjugates and was previously employed to determine immunogenic epitope sugars of parasites, such as Tritrichomonas foetus or Trypanosoma cruzi ( 59 , 60 ). Our data showed a strong binding of the lectins WFA, AAA, ConA, and LEL to TLA, which indicate high abundances of terminal GalNAc, Fuc, Man/Glc, and GlcNAc, respectively.…”

Section: Discussionmentioning

confidence: 99%

Reduction of Allergic Lung Disease by Mucosal Application of Toxoplasma gondii-Derived Molecules: Possible Role of Carbohydrates

et al. 2021

View full text Add to dashboard Cite

BackgroundThe hygiene hypothesis suggests a link between parasitic infections and immune disorders, such as allergic diseases. We previously showed that infection with Toxoplasma gondii or systemic application of T. gondii tachyzoites lysate antigen (TLA) in a prophylactic, but not therapeutic protocol, prevented allergic airway inflammation in mice. Here we tested the effect of prophylactic and therapeutic application of TLA via the mucosal route.MethodsMice were intranasally treated with TLA either i) prior to sensitization, ii) during sensitization and challenge, or iii) after sensitization with ovalbumin (OVA). Recruitment of inflammatory cells to the lung, cytokine levels in restimulated lung and spleen cell cultures as well as levels of OVA-specific antibodies in serum were measured. In parallel, the effect of native TLA, heat-inactivated (hiTLA) or deglycosylated TLA (dgTLA) on sensitized splenocytes was evaluated ex vivo.ResultsWhen applied together with OVA i) during systemic sensitization and local challenge or ii) exclusively during local challenge, TLA reduced infiltration of eosinophils into the lung, OVA-specific type 2 cytokines in restimulated lung cell cultures, and partially, type 2 cytokines in restimulated spleen cell cultures in comparison to allergic controls. No beneficial effect was observed when TLA was applied prior to the start of sensitization. Analysis of epitope sugars on TLA indicated a high abundance of mannose, fucose, N-acetylglucosamine, and N-acetylgalactosamine. Deglycosylation of TLA, but not heat-inactivation, abolished the potential of TLA to reduce type 2 responses ex vivo, suggesting a significant role of carbohydrates in immunomodulation.ConclusionWe showed that mucosal application of TLA reduced the development of experimental allergy in mice. The beneficial effects depended on the timing of the application in relation to the time point of sensitization. Not only co-application, but also therapy in sensitized/allergic animals with native TLA reduced local allergic responses. Furthermore, we show that TLA is highly glycosylated and glycoconjugates seem to play a role in anti-allergic effects. In summary, given the powerful modulatory effect that TLA exhibits, understanding its exact mechanisms of action may lead to the development of novel immunomodulators in clinical application.

show abstract

“…foetus produces a variety of glycoproteins that play a role in parasite binding, bovine epithelial cell damage, and persistence in the reproductive tract (Singh et al, 2004) Specific DNases produced by this protozoon have been characterized that are capable of degrading animal, plant, fungal, and bacterial DNA (Greenwell et al, 2008). Enzyme activity on intact DNA was maximal at pH 4.0 but only 50% of maximum at pH 7.0 (Greenwell et al, 2008). Bacteria present in nutrient media replicate more rapidly than T. foetus and produce metabolic end products that may result in acidic pH, favoring maximal activity of T. foetus DNase's.…”

Section: Discussionmentioning

confidence: 99%

“…This protozoal organism colonizes the mucosa of the penis and prepuce in the bull, producing mild clinical signs and minimal immune recognition (Cobo et al, 2002(Cobo et al, , 2007Greenwell et al, 2008;Ondrak, 2010;Perez et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effects of bacterial contamination of media on the diagnosis of Tritrichomonas foetus by culture and real-time PCR

Clothier

Villanueva

Torain

et al. 2015

Veterinary Parasitology

View full text Add to dashboard Cite

Purification and analysis of DNases of Tritrichomonas foetus: Evidence that these enzymes are glycoproteins

Cited by 9 publications

References 39 publications

Metarhizium anisopliae host–pathogen interaction: differential immunoproteomics reveals proteins involved in the infection process of arthropods

Metarhizium anisopliae host–pathogen interaction: differential immunoproteomics reveals proteins involved in the infection process of arthropods

Reduction of Allergic Lung Disease by Mucosal Application of Toxoplasma gondii-Derived Molecules: Possible Role of Carbohydrates

Effects of bacterial contamination of media on the diagnosis of Tritrichomonas foetus by culture and real-time PCR

Contact Info

Product

Resources

About