Purification and biochemical characterization of two novel extracellular keratinases with feather-degradation and hide-dehairing potential

Kerouaz, Bilal; Jaouadi, Bassem; Brans, Alain; Saoudi, Boudjema; Habbeche, Amina; Haberra, Soumeya; Belghith, Hafedh; Gargroui, Ali; Ladjama, Ali

doi:10.1016/j.procbio.2021.04.009

Cited by 14 publications

(2 citation statements)

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“…Therefore, alkyl sulphate-based IL is an emerging Mar. Drugs 2021, 19, 463 2 of 12 alternative, which is feasible for industrial applications because the synthesis process of halide-free IL can be performed under mild conditions in a one-pot reactor, at reasonable production cost with high yield [9,10]. The alkyl sulphate-based IL exhibits comparatively low viscosity, less toxicity and great biodegradability, resulting in the formation of environmentally benign biphasic system [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Keratinase has been extensively used for diverse industrial applications ranging from animal feeds, organic fertilizers, cleaning agent formulation, leather and textile processing to pharmaceutical and cosmetic supplements [18]. The conventional keratinase purification approaches often require multiple stages of operation to attain desirable purity of keratinase with improved enzyme specificity and catalytic efficiency for commercial applications [19,20]. Therefore, there is a need to develop promising approaches that can effectively recover and purify keratinase with minimal processing time and cost.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Aqueous Biphasic Electrophoresis System Based on Halide-Free Ionic Liquids for Direct Recovery of Keratinase

et al. 2021

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Aqueous biphasic electrophoresis system (ABES) incorporates electric fields into the biphasic system to separate the target biomolecules from crude feedstock. Ionic liquid (IL) is regarded as an excellent candidate as the phase-forming components for ABES because of the great electrical conductivity, which can promote the electromigration of biomolecules in ABES, and thereby enhances the separation efficiency of the target biomolecules from crude feedstock. The application of electric fields to the conventional biphasic system expedites the phase settling time of the biphasic system, which eases the subsequent scaling-up steps and reduces the overall processing time of the recovery process. Alkyl sulphate-based IL is a green and economical halide-free surfactant when compared to the other halide-containing IL. The feasibility of halide-free IL-based ABES to recover Kytococcus sedentarius TWHK01 keratinase was studied. Optimum partition coefficient (Ke = 7.53 ± 0.35) and yield (YT = 80.36% ± 0.71) were recorded with IL-ABES comprised of 15.0% (w/w) [EMIM][ESO4], 20.0% (w/w) sodium carbonate and 15% (w/w) crude feedstock. Selectivity (S) of 5.75 ± 0.27 was obtained with the IL-ABES operated at operation time of 5 min with 10 V voltage supplied. Halide-free IL is proven to be a potential phase-forming component of IL-ABES for large-scale recovery of keratinase.

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