Purification and Characterization of 31-kDa Palm Pollen Glycoprotein (Ela g Bd 31 K), Which is Recognized by IgE from Palm Pollinosis Patients

Structural Features ofN-Glycans Linked to Glycoproteins from Oil Palm Pollen, an Allergenic Pollen*

“…In our previous report, 3) we showed that the putative glycoallergen, Ela g Bd 31 K, recognized by IgE from the palm pollinosis bears the antigenic complex type N-glycan(s) containing b1-2 xylose or a1-3 fucose residues. It has been reported that recognition of the b1-2 xylose residue by IgE of pollen allergenic patients would be dependent on the degree of substitution of the core mannose in‰uencing the conformation of the epitope.…”

“…1,2) In our previous paper, 3) we succeeded in purifying and identifying a glycoprotein with a molecular mass of 31 kDa from oil palm pollens, which was recognized by antisera from palm pollinosis patients, suggesting that this glycoprotein (Ela g Bd 31 K) might be one of allergens in the oil palm pollen. Immunoblotting analysis with antiserum against the b1-2 xylose residue showed that the glycoprotein bears the plant complex type N-glycan, which has a strong antigenicity for mammals and therefore has been postulated as an epitope for IgE from pollen allergy or plant food allergy patients.…”

Aerobiological, clinical and immunobiochemical studies on Lantana camara pollen and cross-reactivity with other Verbenaceae pollen species

“…A study on four species of Cassia showed that higher level of carbohydrates (11-15%) than lipids (8-12%) (Mondal et al 1998). There have been several reports where glycoproteins, polysaccharides and lipoproteins serve as effective allergens in pollen grains and food stuffs (Nilsen and Paulsen 1990;Batanero et al 1996;Kimura et al 2002;Gómez-Gómez et al 2010;Gadermaier et al 2011, Kuppannan et al 2011.…”

Sensitization to oil palm pollen associates with risks and severity of allergic diseases

“…Oil palm sensitization is an important risk factor of asthma and AR in the Southeast Asian tropical region. 6 , 13 Multiple oil palm pollen proteins have been characterized and showed IgE reactivity, including a 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), 14 a pectin esterase-like oil palm pollen protein designated as ELGU1, 15 as well as a polygalacturonase-like protein. 16 While the allergenicity of these proteins has been assessed previously, the specific role of oil palm sensitization in the manifestation of allergic diseases remains to be investigated.…”