Purification and characterization of a novel laccase from Fomitopsis pinicola mycelia

Park, Naomi; Park, Sang‐Shin

doi:10.1016/j.ijbiomac.2014.06.019

Cited by 37 publications

(18 citation statements)

References 36 publications

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“…Similar result had been reported for M. albomyces laccase [23]. However, EDTA had an inhibitory effect on most of characterized fungal laccases [42,43]. The resistance against this chelator agent was explained by its low accessibility to the structural copper atoms present in the active site, required for catalytic enzyme activity [3].…”

Section: Effect Of Inhibitors On Laccase Activitysupporting

confidence: 81%

Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye

Mtibaà

Barriuso

Eugenio

et al. 2018

International Journal of Biological Macromolecules

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Section: Effect Of Inhibitors On Laccase Activitysupporting

confidence: 81%

Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye

Mtibaà

Barriuso

Eugenio

et al. 2018

International Journal of Biological Macromolecules

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“…Surprisingly, no laccase activities were detected for F . pinicola alone (in single-species cultures) on any of the culture media tested, although laccase-like oxidoreductase (ABTS oxidation) activity and protein expression was reported for another isolate of the species [ 57 ], and three laccase encoding genes have been predicted from F . pinicola genome sequence [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Interactions affect hyphal growth and enzyme profiles in combinations of coniferous wood-decaying fungi of Agaricomycetes

et al. 2017

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Fomitopsis pinicola is a species of Polyporales frequently encountered in Nordic temperate and boreal forests. In nature, the fungus causes destructive brown rot in wood, colonizing tree trunks often occupied by other Basidiomycota species. We mimicked these species-species interactions by introducing F. pinicola to five white rot species, all common saprotrophs of Norway spruce. Hyphal interactions and mycelial growth in various combinations were recorded, while activities of lignocellulose-acting CAZymes and oxidoreductases were followed in co-cultures on two different carbon-source media. Of the species, Phlebia radiata and Trichaptum abietinum were the strongest producers of lignin-modifying oxidoreductases (laccase, manganese peroxidase) when evaluated alone, as well as in co-cultures, on the two different growth media (low-nitrogen liquid medium containing ground coniferous wood, and malt extract broth). F. pinicola was an outstanding producer of oxalic acid (up to 61 mM), whereas presence of P. radiata prevented acidification of the growth environment in the liquid malt-extract cultures. When enzyme profiles of the species combinations were clustered, time-dependent changes were observed on wood-supplemented medium during the eight weeks of growth. End-point acidity and production of mycelium, oxalic acid and oxidoreductase activities, in turn clustered the fungal combinations into three distinct functional groups, determined by the presence of F. pinicola and P. radiata, by principal component analysis. Our findings indicate that combinations of wood-decay fungi have dramatic dynamic effects on the production of lignocellulose-active enzymes, which may lead to divergent degradative processes of dead wood and forest litter.

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“…POD class II has been reported as a single copy in P. placenta, W. cocos and F. pinicola genomes (31), and the P. placenta peroxidase (Ppl44056) was classi ed as a basal peroxidase, not closely related to LiP and MnP (45). Laccases in Polyporales are multigenic (46) and have been characterized as functional enzymes in P. placenta and F. pinicola (47)(48)(49), playing a role in wood decay performed by P. placenta (47). Signi cant lignolysis has been observed in Gloeophyllum trabeum (Gloeophyllales) and P. placenta without considering the involvement of PODs class II (6,50).…”

Section: Discussionmentioning

confidence: 99%

Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600

Oliveira

Figueiredo

Gonçalves

et al. 2020

Preprint

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Background: Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant substrates. Brown-rot strains produce carbohydrate-active enzymes involved in the degradation of lignocellulosic materials, along with a non-enzymatic mechanism via Fenton reaction. Differences in the lignocellulose metabolism among closely related brown rots are not completely understood. Here, a multi-omics approach provided a global understanding of the strategies employed by L. sulphureus ATCC 52600 in the degradation of lignocellulosic by-products derived from sugarcane and Eucalyptus. Results: To evidence the oxidative-hydrolytic mechanism, the Laetiporus sulphureus ATCC 52600 genome was sequenced and the response to lignocellulosic substrates was analyzed by transcriptomics and proteomics. The transcriptomic profile in response to a short cultivation period on in natura sugarcane bagasse revealed 128 out of 12,802 upregulated transcripts. The high upregulated transcripts included a set of redox enzymes along with hemicellulases. The exoproteome produced in response to extended time cultivation on Avicel, and steam-exploded sugarcane bagasse, sugarcane straw, and Eucalyptus (from Eucalyptus grandis) revealed 121 proteins. Contrasting with the mainly oxidative profile observed in the transcriptome, the secretomes showed a diverse hydrolytic repertoire including constitutive cellulases and hemicellulases, in addition to 19 upregulated proteins relative to glucose. The secretome produced on sugarcane bagasse was evaluated in the saccharification of pretreated sugarcane straw by supplementing a commercial cocktail. Additionally, growth analysis revealed that L. sulphureus ATCC 52600 has higher efficiency to assimilate glucose than other mono- and disaccharides.Conclusion: This study shows the singularity of L. sulphureus ATCC 52600 compared to other Polyporales brown rots, regarding the presence of cellobiohydrolase and peroxidase class II. The multi-omics analysis reinforces the oxidative-hydrolytic metabolism involved in lignocellulose deconstruction, providing insights into the overall mechanisms as well as specific proteins of each step.

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Purification and characterization of a novel laccase from Fomitopsis pinicola mycelia

Cited by 37 publications

References 36 publications

Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye

Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye

Interactions affect hyphal growth and enzyme profiles in combinations of coniferous wood-decaying fungi of Agaricomycetes

Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600

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