1994
DOI: 10.1111/j.1432-1033.1994.00243.x
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Purification and Characterization of a Ribonuclease from Aspergillus giganteus IFO 5818, the Gigantin

Abstract: A protein has been isolated from a culture medium of Aspergillus giganteus I F 0 5818 (gigantin) and purified by ion-exchange chromatography successively on DEAE-cellulose and carboxymethylcellulose, and gel-filtration chromatography on Biogel P10. With a high purity, gigantin was found to be a non-glycosylated basic protein with a relative molecular mass of 17000 2 200 determined in PAGEEDS. Gigantin was able to digest the synthetic homopolymers of nucleic acids poly(A), poly(I), poly(C) and poly(U). The cata… Show more

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Cited by 8 publications
(2 citation statements)
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“…Although ribotoxin production has also been observed in A. restrictus isolates NRRL 2869 (named restrictocin; Olson et al 1963 ; Yang and Kenealy 1992 ; López-Otín et al 1984 ) and NRRL 3050 (named regulin; Olson and Goerner 1966 ), these isolates have later been re-identified as A. fumigatus (Summerbell 2002 ). Several other ribotoxins have been identified since then, including mitogillin (Rodriguez et al 1982 ; Fernandez-Luna et al 1985 ), clavin (Parente et al 1996 ), gigantin (Salvarelli et al 1994 ; Wirth et al 1997 ), c-sarcin (Huang et al 1997 ), and hirsutellins (Mazet and Vey 1995 ; Liu et al 1995 ; Martínez-Ruiz et al 1999b ), a homolog of which have also been identified in the genomic DNA sequence of A. clavatus NRRL 1 (WC Nierman, unpublished; Genbank accession number XM_001273330). Aspf1, another ribotoxin produced by A. fumigatus has also been identified as a major allergen in Aspergillus -related diseases (Arruda et al 1992 ).…”
mentioning
confidence: 99%
“…Although ribotoxin production has also been observed in A. restrictus isolates NRRL 2869 (named restrictocin; Olson et al 1963 ; Yang and Kenealy 1992 ; López-Otín et al 1984 ) and NRRL 3050 (named regulin; Olson and Goerner 1966 ), these isolates have later been re-identified as A. fumigatus (Summerbell 2002 ). Several other ribotoxins have been identified since then, including mitogillin (Rodriguez et al 1982 ; Fernandez-Luna et al 1985 ), clavin (Parente et al 1996 ), gigantin (Salvarelli et al 1994 ; Wirth et al 1997 ), c-sarcin (Huang et al 1997 ), and hirsutellins (Mazet and Vey 1995 ; Liu et al 1995 ; Martínez-Ruiz et al 1999b ), a homolog of which have also been identified in the genomic DNA sequence of A. clavatus NRRL 1 (WC Nierman, unpublished; Genbank accession number XM_001273330). Aspf1, another ribotoxin produced by A. fumigatus has also been identified as a major allergen in Aspergillus -related diseases (Arruda et al 1992 ).…”
mentioning
confidence: 99%
“…␣-Sarcin is a cytotoxic protein belonging to the ribotoxins, a family of ribosome-inactivating proteins (2,6,7,13,17,24,30). AFP is a highly basic polypeptide of 51 amino acids with a high content of cysteine, tyrosine, and lysine residues.…”
mentioning
confidence: 99%