Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus

Arita, Michiko

doi:10.1016/0378-1097(91)90536-j

Cited by 46 publications

(56 citation statements)

References 18 publications

(12 reference statements)

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“…However, most NAG vibrios do not produce these enterotoxins. A heatstable enterotoxin of NAG vibrio was purified and characterized by Arita et al in 1986, and they called it NAG-ST (2). This enterotoxin appeared to be more widely distributed among NAG vibrios.…”

mentioning

confidence: 99%

An Aberrant Hemolysin of Vibrio cholerae Non‐O1

Iwanaga

Ichinose

1991

Microbiology and Immunology

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An aberrant hemolysin produced by a Vibrio cholerae non-O1 strain NO37 (NO37-hly) was purified and characterized. NO37-Hly was antigenically very similar to El Tor hemolysin but differed in molecular weight (48,000 vs. 60,000), interaction with glucose, and hemolytic activity. Of 100 V. cholerae non-O1 strains other than the NO37 strain examined, none produced this aberrant hemolysin. The N-terminal amino acid sequence of NO37-hly was highly homologous to that of El Tor hemolysin.

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confidence: 99%

An Aberrant Hemolysin of Vibrio cholerae Non‐O1

Iwanaga

Ichinose

1991

Microbiology and Immunology

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“…The SMA is, obviously, not a suitable reference assay to evaluate the ELISA for detection of NAG-ST. Heating of the culture supernatant of V. cholerae non-Ol at 100 C for 5 min to eliminate the heat-labile hemolysis before introducing into the sucking mice may not solve the problem owing to the reported heat fragility of the NAG-ST in its crude state (1). The NAG-ST competitive ELISA developed in this study cannot be used for routine screening of broth cultures of V. cholerae non-Ol.…”

Section: Discussionmentioning

confidence: 94%

Identification of Heat‐Stable Enterotoxin‐Producing Strains of Yersinia enterocolitica and Vibrio cholerae Non‐O1 by a Monoclonal Antibody‐Based Enzyme‐Linked Immunosorbent Assay

Nair

Bhattacharya

Takeda³

1993

Microbiology and Immunology

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Using a mouse monoclonal antibody (MAb) 2F raised against Vibrio cholerae non-01 heat-stable enterotoxin (NAG-ST) which also recognizes a shared epitope of Yersinia enterocolitica heat-stable enterotoxin (Y-ST), a competitive enzyme-linked immunosorbent assay (ELISA) was developed for independent detection of NAG-ST and Y-ST. There was good concordance between the Y-ST ELISA and the suckling mouse assay (SMA) for detection of Y-ST from test strains of Y. enterocolitica, and the Y-ST ELISA can effectively replace the SMA for routine detection of Y-ST. On the contrary, evaluation of the NAG-ST ELISA and the SMA using 139 strains of V. cholerae non-01 showed discordant results and this was attributed to the presence of the suckling mice active factor(s) such as El Tor hemolysin and to the production of low amounts of NAG-ST. Concentration of culture supernatants of V. cholerae non-01 followed by heating at 100 C was essential to obtain reproducible results by both the NAG-ST ELISA and the SMA. The ELISA developed in this study can be used for the identification of biologically active strains. While recently genetic methods such as polymerase chain reaction became available and were very reliable and simple techniques, the ELISA in this study has an advantage in detecting biologically toxic gene products of the strains. The genetic methods cannot differentiate silent STa genes which we often encounter in the case of Y. enterocolitica.

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“…The V. mimicus strains used in this study were isolated from patients with diarrhoea including 5 sporadic cases, 4 cases of food poisoning and 4 overseas travellers, those from environment sources included 15 water samples and 14 live fish, and those from purchased sea-foods. The V. mimicus isolates were identified by standard biochemical tests [1].…”

Section: Strains Of V Mimicusmentioning

confidence: 99%

“…Gastroenteritis due to V. mimicus has occurred following ingestion of seafoods [1,2]. Some V. mimicus strains have been reported to produce toxins similar to cholera enterotoxin (CT) [3] and heat-stable enterotoxin (ST) [4]. However, the frequency of such toxin-producing strains was estimated to be less than 10 % of both clinical and environmental isolates [1,5].…”

Section: Introductionmentioning

confidence: 99%

High prevalence of thermostable direct hemolysin (TDH)-like toxin inVibrio mimicusstrains isolated from diarrhoeal patients

et al. 1993

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SUMMARYA total of 17 isolates of Vibrio mimicus from patients, 29 from environment and 2 from food was examined for toxigenicity. Sixteen (94 %) clinical isolates and one (50%) from food produced TDH-like toxin, whereas none of the environmental isolates did so. The food from which V. mimicus with TDH-like toxin production was isolated, was one which had caused food poisoning. Only one environmental strain produced CT-like toxin, whilst ST-like toxin was not detected from any strains tested.

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Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus

Cited by 46 publications

References 18 publications

An Aberrant Hemolysin of Vibrio cholerae Non‐O1

An Aberrant Hemolysin of Vibrio cholerae Non‐O1

Identification of Heat‐Stable Enterotoxin‐Producing Strains of Yersinia enterocolitica and Vibrio cholerae Non‐O1 by a Monoclonal Antibody‐Based Enzyme‐Linked Immunosorbent Assay

High prevalence of thermostable direct hemolysin (TDH)-like toxin inVibrio mimicusstrains isolated from diarrhoeal patients

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