Purification and Characterization of a Lipase fromAspergillus oryzae

Production of Fungal Biomass for Feed, Fatty Acids, and Glycerol by Aspergillus oryzae from Fat-Rich Dairy Substrates

“…Differences in glycerol concentration were more evident up to pH 6 than within the pH interval 6-7 ( Figure 1). Thus, at the examined conditions, production of lipase by A. oryzae was favored at neutral pH, which is in agreement with previous studies [14]. Neutral pH has also been observed to induce the highest lipase activity by A. flavus, while a pH of 6 was reported to induce the highest lipase activity in A. niger [15].…”

“…The present research adds further insights into the biological conversion of dairy substrates by A. oryzae, since its lactase was found to have the highest activity within the pH range of 4-6 [3]. Furthermore, a temperature range of 30-37 °C is reported to induce maximal lipase activity by Aspergillus species [13][14][15]. Since the pH had not been kept constant during cultivation, a further experiment was carried out to establish a relationship between pH value and biomass production over time ( Figure 2).…”

“…The final concentration of palmitic (4.56 ± 0.44 g/L) and myristic (3.68 ± 0.08 g/L) acids were considerably higher than that of stearic and oleic acids (less than 1 g/L of each). This difference in the release of fatty acids may be due to the type and activity of the lipase produced by A. oryzae [14,16]. Lipases are selective on the substrate; therefore, the final product depends on whether the initial substrates are more concentrated in tri-, di-, or mono-glycerides [17].…”

Isolation of lipase producing fungi from palm oil Mill effluent (POME) dump sites at Nsukka

“…Growth and multiplication of microorganisms on any substrate is often considered as the first step towards its bioconversion (Molla et al, 2002). The fungal organisms isolated were identical to those earlier reported by Ibrik et al, (1995); Ugoji (1997); Toida et al, (1998); Namboodiri and Chattopadhaya (2000) and Savitha et al, (2007) The use of fungal lipases to facilitate processes and application is gaining grounds and so many of them have been characterized (Bapiraju et al, 2004, Prazeres et al, 2006, Kashmiri et al, 2006and Savitha et al, 2007. In the area of waste water treatment, especially the bioremediation of palm oil mill effluent (POME), the yeast Yarrowia lipolytica have been successfully utilized.…”

Efficient Expression of Mono- and Diacylglycerol Lipase Gene fromPenicillium camembertiiU-150 inAspergillus oryzaeunder the Control of Its Own Promoter

“…Since the full promoter sequences of other fungal lipase genes were not reported except for these three genes, no further comparison could be done. A strain of A. oryzae was reported to produce a MDGL-like enzyme 26 ) but the presence of lipid induction of the lipase expression has not been examined in the A. oryzae strain. A. oryzae strain used as a host in this study did not produce such a MDGL-like enzyme under the conditions we used.…”