Purification and characterization of alkaline phosphatase from lactic acid bacteria

Chu, Yuhao; Yu, Xin-Xin; Jin, Xing; Wang, Yutang; Zhao, Duo-Jia; Zhang, Po; Sun, Guang-Mei; Zhang, Yinghua

doi:10.1039/c8ra08921c

Cited by 36 publications

(14 citation statements)

References 36 publications

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“…The conversion from the insoluble to the soluble phase-as described before [13]-is facilitated at the interphase between an inorganic and an organic phase like with bacteria residing in/on the cement. Also associated with bacteria is the ALP, an enzyme that degrades polyP to P i [33,38]. Ca 2+ is known to accumulate in cement and to leach out from this material [39].…”

Section: Discussionmentioning

confidence: 99%

Self-Healing Properties of Bioinspired Amorphous CaCO3/Polyphosphate-Supplemented Cement

Tolba

Wang

et al. 2020

Molecules

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There is a strong interest in cement additives that are able to prevent or mitigate the adverse effects of cracks in concrete that cause corrosion of the reinforcement. Inorganic polyphosphate (polyP), a natural polymer that is synthesized by bacteria, even those on cement/concrete, can increase the resistance of concrete to progressive damage from micro-cracking. Here we use a novel bioinspired strategy based on polyP-stabilized amorphous calcium carbonate (ACC) to give this material self-healing properties. Portland cement was supplemented with ACC nanoparticles which were stabilized with 10% (w/w) Na–polyP. Embedding these particles in the hydrated cement resulted in the formation of calcite crystals after a hardening time of 10 days, which were not seen in controls, indicating that the particles dissolve and then transform into calcite. While there was no significant repair in the controls without ACC, almost complete closure of the cracks was observed after a 10 days healing period in the ACC-supplemented samples. Nanoindentation measurements on the self-healed crack surfaces showed a similar or slightly higher elasticity at a lower hardness compared to non-cracked surfaces. Our results demonstrate that bioinspired approaches, like the use of polyP-stabilized ACC shown here, can significantly improve the repair capacity of Portland cement.

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Section: Discussionmentioning

confidence: 99%

Self-Healing Properties of Bioinspired Amorphous CaCO3/Polyphosphate-Supplemented Cement

Tolba

Wang

et al. 2020

Molecules

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“…The Caco-2 cells were dissolved with 1% Triton X-100 (1 mL per well), and the collected lysate stored at -20 °C. Then the alkaline phosphatase (AKP, BC2145, Solarbio, BeiJing, China) [ 21 ] and lactate dehydrogenase (LDH, BC0685, Solarbio, BeiJing, China) activities were tested according to the instructions of the sword test box. Three parallel experiments were performed in each group.…”

Section: Methodsmentioning

confidence: 99%

“…The components are as follows: The suspension of CT group (1 mL sterile PBS), LAB group (1 mL 2 × 10 8 CFU/mL Lactobacillus), SAL group (1 mL 2 × 10 8 CFU/mL Salmonella), and rejection group (1 mL of Lactobacillus was cultured for 1 h, then 1 mL of Salmonella was cultured for 1 h), each group was cultured for 2 h. After culture, the supernatant was collected, and the Caco-2 cells were washed three times with sterile PBS. The Caco-2 cells were dissolved with 1% Triton X-100 (1 mL per well), and the collected lysate stored at −20 • C. Then the alkaline phosphatase (AKP, BC2145, Solarbio, BeiJing, China) [21] and lactate dehydrogenase (LDH, BC0685, Solarbio, BeiJing, China) activities were tested according to the instructions of the sword test box. Three parallel experiments were performed in each group.…”

Section: Effect Of Lactobacillus Adhesion To Caco-2 Cells On Their Phmentioning

confidence: 99%

In Vitro Screening of Chicken-Derived Lactobacillus Strains that Effectively Inhibit Salmonella Colonization and Adhesion

Hai

Huang

et al. 2021

Foods

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Inhibition of Salmonella by Lactobacillus has been a popular research topic for decades; however, the inhibition potential of chicken-derived Salmonella by chicken-derived Lactobacillus has not yet been studied. In this study, 89 strains of Lactobacillus from chicken intestines were isolated by national standard method, Gram staining, physiological, and biochemical experiments and molecular sequencing; The inhibition characteristics of 89 strains of chicken derived Lactobacillus against 10 strains Salmonella (S. Enteritidis SE05, SC31, SC21, SC72 SC74, SC79, SC83, SC87; S. bongori SE47; S. Typhimurium, SC85) were detected by agar inhibition zone, The results showed that the inhibition zone of 24 strains of chicken derived Lactobacillus was more than 10 mm, which indicated that the isolated chicken derived Lactobacillus could effectively inhibit the growth of Salmonella; The drug resistance and bile salt tolerance of these 24 strains were analyzed, The results showed that the standard strains LG and L76 were not resistant, and the other 22 Lactobacillus strains showed different degrees of resistance. The strains LAB24, LAB26, LAB53, LAB69, and L76 showed good tolerance at the concentration of 3 g/L bile salt; Caco-2 cell experiment and flow cytometry were used to analyze the inhibitory effect of chicken derived Lactobacillus on the adhesion of Salmonella to Caco-2 cells, The results showed that 16 probiotics could effectively inhibit the adhesion of Salmonella to Caco-2 cells. Twelve probiotics were identified by molecular biology. The results showed that L76 was Enterococcus faecalis, and the other 11 strains were Lactobacillus.

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“…Alkaline phosphatase from lactic acid bacteria was purified by ion‐exchange chromatography. The findings of the study reveal that alkaline phosphatase could be used in the clean‐up of pesticides in raw materials used in the food industry [28].…”

Section: Techniques To Study Comparative Proteomicsmentioning

confidence: 99%

Potential of proteomics to probe microbes

et al. 2020

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An organism exposed to a plethora of environmental perturbations undergoes proteomic changes which enable the characterization of total proteins in it. Much of the proteomic information is obtained from genomic data. Additional information on the proteome such as posttranslational modifications, protein–protein interactions, protein localization, metabolic pathways, and so on are deduced using proteomic tools which genomics and transcriptomics fail to offer. The proteomic analysis allows identification of precise changes in proteins, which in turn solve the complexity of microbial population providing insights into the microbial metabolism, cellular pathways, and behavior of microorganisms in new environments. Furthermore, they provide clues for the exploitation of their special features for biotechnological applications. Numerous techniques for the analysis of microbial proteome such as electrophoretic, chromatographic, mass spectrometric‐based methods as well as quantitative proteomics are available which facilitate protein separation, expression, identification, and quantification of proteins. An understanding of the potential of each of the proteomic tools has created a significant impact on diverse microbiological aspects and the same has been discussed in this review.

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Purification and characterization of alkaline phosphatase from lactic acid bacteria

Abstract: Alkaline phosphatase (ALP) excreted from lactic acid bacteria (LAB) showed the ability to degrade organophosphorus pesticides.

Cited by 36 publications

References 36 publications

Self-Healing Properties of Bioinspired Amorphous CaCO3/Polyphosphate-Supplemented Cement

Self-Healing Properties of Bioinspired Amorphous CaCO3/Polyphosphate-Supplemented Cement

In Vitro Screening of Chicken-Derived Lactobacillus Strains that Effectively Inhibit Salmonella Colonization and Adhesion

Potential of proteomics to probe microbes

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