Purification and characterization of allergens from Xanthium strumarium pollen

Jaggi, Kuldeep S.; Gangal, S. V.

doi:10.1007/bf00229692

Cited by 13 publications

(6 citation statements)

References 11 publications

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“…are well-known allergenic pollens (Newmark and Itkin 1967;Erickson et al 1984;Bousquet et al 1986;Jaggi and Sharad 1987). In our study, they were also present in high values in the atmosphere of Istanbul.…”

Section: Seasonssupporting

confidence: 53%

Airborne pollen in European and Asian parts of Istanbul

Çelenk

Bıçakçı

Tamay

et al. 2009

Environ Monit Assess

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Pollen concentrations in the atmosphere of Istanbul, a city located between two continents, has been monitored for 1 year as part of a larger research program. The sampling sites were located in two different continents: the Asian part (AS) and the European part (EP). The sampling was performed in AS and EP of the city by using Hirst type volumetric method, and pollen grains of 58 and 62 taxa were identified in the two parts, respectively. The pollen spectrum reflected the floristic diversity of the region. The main pollen producers at the sites were characterized by some allergenic pollen and were identified as Cupressaceae/Taxaceae, Urticaceae, Pistacia sp., Quercus sp., Platanus sp., Fraxinus sp., and Xanthium sp. These pollen types contributed to the total pollen sum with a percentage of more than 80% at both monitoring sites. The highest amount of pollen grains was recorded in April. The greatest number of species was recorded in May, when 42 types (AS) and 44 types (EP) were present.

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Section: Seasonssupporting

confidence: 53%

Airborne pollen in European and Asian parts of Istanbul

Çelenk

Bıçakçı

Tamay

et al. 2009

Environ Monit Assess

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“…Rhiz VIb was found to be stable on heating and was also resistant to enzymatic digestion with all the three enzymes. Similar findings have been reported in Alternaria basic fraction (5), castor bean allergen (27) and Xanthium pollen (13).…”

Section: Discussionsupporting

confidence: 89%

Immunocheraical investigation of allergens from Rhizopus nigricans

Sridhara¹,

Gangal²,

Joshi³

1990

Allergy

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The allergenic proteins of mould, Rhizopus nigricans extract (RNE) were identified and characterized by crossed immunoelectrophoresis (CIE), thin-layer isoelectrofocusing (TLIEF) and RAST inhibition. CIE revealed that the extract contained at least 31 distinct antigens. On TLIEF the extract resolved into 22 bands in pI 3.5-6.8. Two important allergens, Rhiz IIIb and VIb were purified by a combination of ammonium sulfate precipitation, anion exchange chromatography on DEAE-Sephadex column and gel filtration. Twenty and 12 micrograms of Rhiz IIIb and Rhiz VIb were sufficient to give 50% RAST inhibition as against 43 micrograms of crude RNE. Rhiz IIIb and Rhiz VIb were found to be glycoproteins with molecular weights of 12,400 daltons and 14,200 daltons, respectively. Rhiz IIIb was found to be homogeneous on polyacrylamide gel electrophoresis (PAGE) and TLIEF with a pI of 4.8, while Rhiz VIb gave a single band on PAGE and resolved into two Coomassie blue stained bands with pI 3.6 and 3.8. It was possible to separate the components of RNE on fast protein liquid chromatography (FPLC) using an anion exchanger Mono Q column. The identification and characterization of the antigenic and allergenic proteins in the extract will be useful in standardization of RNE and in preparation of an in-house reference standard.

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“…Thus, a major allergenic protein, Bras 2a MW 90 kD, has been isolated and identified in BC pollen extract. In India, studies with Amaranthusspinosus, Xanthium strumarium (weed pollen) and Cocos nucifera (tree pollen) have revealed the glycoproteins of higher MW (128, 103, 116-200 kD, respectively) as major allergenic proteins [18][19][20]. However, with other pollen antigens, such as Ageratum conyzoides, Artemisia scoparia, Cynodon dactylon and Prosopis julijlora, low MW proteins of 10-32 kD have been recorded as major allergenic components [21 24].…”

Section: Discussionmentioning

confidence: 99%

Immunobiochemical Characterization of <i>Brassica campestri</i><i>s</i> Pollen Allergen

Singh

Verma²,

Rai³

et al. 1995

Int Arch Allergy Immunol

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Brαssica campestris (BC), Eng. Mustard, is an important source of pollen allergen, responsible for type I hypersensitivity disorders. In the present study, BC pollen extract was characterized by TLIEF, SDS-PAGE and immunoprinting. The extract separated into 50 silver stained bands of pi 3-9 on isoelectric focusing whereas it resolved into 14 Coomassie blue stained protein bands of 14–100 kD on SDS-PAGE. Immunoblot analysis with individual patient sera detected four allergenic proteins of 90, 67, 60 and 14 kD. BC separated into 8 peaks (Bras 1–8) on DEAE Sephadex A-50 column. Bras 2 was found to be most potent by IgE specific ELISA, hence further fractionated on Sephadex G-200. A protein of 90 kD (Bras 2a) isolated by gel filtration was found to be most allergenic protein by ELISA inhibition. The findings shall be applicable in standardization of future batches of BC pollen extract to be used for allergy diagnosis and immunotherapy.

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Purification and characterization of allergens from Xanthium strumarium pollen

Cited by 13 publications

References 11 publications

Airborne pollen in European and Asian parts of Istanbul

Airborne pollen in European and Asian parts of Istanbul

Immunocheraical investigation of allergens from Rhizopus nigricans

Immunobiochemical Characterization of <i>Brassica campestri</i><i>s</i> Pollen Allergen

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