Purification and characterization of carboxymethylcellulase isolated from a marine bacterium, Bacillus subtilis subsp. subtilis A-53

Kim, Bokyung; Lee, Bo-Hwa; Lee, Yoo-Jung; Jin, Il-Hyuck; Chung, Chong-Pyoung; Lee, Jin Woo

doi:10.1016/j.enzmictec.2009.02.005

Cited by 109 publications

(71 citation statements)

References 33 publications

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“…YJ5 showing its optimum pH of 7 and pH stability range of 7.5-10.5. Cellulase enzyme of B. subtilis subsp subtilis A-54 has optimum pH of 6.5 and stable in pH range of 6.5-8 [33]. According to previous studies, cellulases are active at the pH range of 6.0 to 7.0 from A. Niger [34], 5.0 to 7.0 from Lysobacter sp.…”

Section: Effect Of Ph On Activity and Stability Of Cellulasementioning

confidence: 99%

Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity

Irfan¹,

Safdar²,

Syed³

et al. 2012

Turk J Bioch

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Section: Effect Of Ph On Activity and Stability Of Cellulasementioning

confidence: 99%

Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity

Irfan¹,

Safdar²,

Syed³

et al. 2012

Turk J Bioch

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“…Activity of the CMCase produced by E. coli JM109/DL-3 was determined based on the release of reducing sugar from CMC using the 3,5-dinitrosalicylic acid (DNS) method, as described in the previous report [14]. Glucose (Sigma-Aldrich, UK) was used to prepare a calibration curve.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Lee¹,

Kim²,

Gao³

et al. 2011

Journal of Life Science

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The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that K2HPO4 gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was K2HPO4 ("probe>F" less than 0.0001), whereas K2HPO4 and MgSO4•7H2O were significant for the production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.

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“…subtilis A-53 by Kim, which indicated certain Bacillus cereus sp. take part in the biodegradation process of cellulose in the natural world [20]. Interestingly, a large number of Bacillus strains have important economic relevance are used as probiotics, besides members of the genus was attributed to pathogens for agricultural application [21,22].…”

Section: Molecular Identification Of Strain Yc-a1mentioning

confidence: 99%

Characterization of a Cellulose and Xylose Utilization Bacillus cereus Yc-A1

Zhang¹,

Zhu²,

Yang³

et al. 2015

Proceedings of the International Conference on Advances in Energy, Environment and Chemical Engineering

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Purification and characterization of carboxymethylcellulase isolated from a marine bacterium, Bacillus subtilis subsp. subtilis A-53

Cited by 109 publications

References 33 publications

Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity

Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Characterization of a Cellulose and Xylose Utilization Bacillus cereus Yc-A1

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