Purification and characterization of extracellular β-galactosidase from the psychrotolerant yeast Guehomyces pullulans 17-1 isolated from sea sediment in Antarctica

Song, Chunli; Liu, Guanglei; Xu, Jin-Li; Chi, Zhen‐Ming

doi:10.1016/j.procbio.2010.02.025

Cited by 35 publications

(24 citation statements)

References 26 publications

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“…This functional diversity is due, in part, to the fact that yeasts have extraordinary metabolic potential. This potential is available for exploitation [15][16][17][18][19][20], but notably, the vast majority of this potential has yet to be discovered. Several yeast compounds have significant biological value as reagents, cell proteins, vitamins, pigments, and enzymes.…”

Section: Introductionmentioning

confidence: 99%

Use of Yeasts as Probiotics in Fish Aquaculture

Navarrete¹,

Tovar‐Ramírez²

2014

Sustainable Aquaculture Techniques

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Yeasts are unicellular eukaryotic microorganisms that are taxonomically placed within the phyla Ascomycota and Basidiomycota within the Kingdom Fungi [7]. Ascomycete yeasts comprise approximately 1,000 phylogenetically diverse species that have recently been assigned to 14 different lineages on the basis of multigene sequence analysis [8]. The other species of yeasts are classified as the basidiomycetes [9]. Some of the general characteristics and ecological properties of each phylum include the following: 1) the cell wall polysaccharide composition is dominated by chitin in the basidiomycetes and by β-glucans in the ascomycetes; 2) the guanine + cytosine (G + C) composition of the nuclear DNA tends to be higher than 50% in basidiomycetes and lower than 50% in ascomycetes; 3) ascomycetes yeasts are generally more fermentative, more copiotrophic (but at the same time nutritionally specialized), more fragrant, and mostly hyaline, while basidiomycete yeasts more often form mucoid colonies, Sustainable Aquaculture Techniques 136 Marine samples Identified yeast Identification method Reference

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Section: Introductionmentioning

confidence: 99%

Use of Yeasts as Probiotics in Fish Aquaculture

Navarrete¹,

Tovar‐Ramírez²

2014

Sustainable Aquaculture Techniques

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“…The hydrolysis of lactose by ␤-galactosidase can be used to improve digestibility, solubility, and sweetness of dairy products [7][8][9]. ␤-Gal was traditionally purified from a variety of sources by ammonium sulfate precipitation and different chromatography techniques [10][11][12][13][14][15][16][17]. These methods involve a large number of steps, which make them time consuming and difficult to scale up.…”

Section: Introductionmentioning

confidence: 99%

Production, recovery and purification of a recombinant β-galactosidase by expanded bed anion exchange adsorption

Boeris

Balce

Vennapusa

et al. 2012

Journal of Chromatography B

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“…Namely, changes in lipid metabolism of cold-adapted yeasts constitute the major adaptation of metabolic functions occurring during growth at low temperatures. [5][6][7][8][9][10] Composition of lipids is also very important for the quality of biodiesel. Saturated fatty acids (such as C16:0 and C18:0) and monounsaturated fatty acids (such as C16:1 and C18:1) are generally preferred for biodiesel were centrifuged at 5,000 rpm for 5 min.…”

mentioning

confidence: 99%

Microbial lipid production by cold‐adapted oleaginous yeast Yarrowia lipolytica B9 in non‐sterile whey medium

Taşkın

Saghafian

Aydoğan

et al. 2015

Biofuels Bioprod Bioref

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Deproteinized whey was used as a substrate for the production of lipids by cold‐adapted yeast Yarrowia lipolytica B9 under non‐sterile culture conditions. Undesired microbial contamination in non‐sterile whey medium could be prevented when appropriate culture parameters (inoculum size of 3 mL/100 mL, initial pH of 5.5 and incubation temperature of 15 °C) were selected. In contrast to additional nitrogen (ammonium sulfate) and phosphorus (potassium dihydrogen phosphate) sources, additional carbon source (lactose) increased lipid accumulation. Under optimized culture conditions, biomass and lipid concentrations of the yeast were found as 7.4 g/L and 4.29 g/L, respectively. Lipid content was determined as 58% of total cell biomass. Fatty acids of the yeast were oleic acid (18:1), cis‐10‐heptadecenoic acid (C17:1), palmitoleic acid (16:1) and palmitic acid (16:0). The yeast was found to contain no polyunsaturated fatty acids. The content of C16 and C18 fatty acids was found to be 91.98% of total lipids. Monounsaturated fatty acids accounted for 80.54% of total lipids. Due to rich monounsaturated fatty acid composition, biomass of Y. lipolytica B9 may be used as feedstock for biodiesel production, especially operating in winter conditions. This is the first report on the use of cheese whey as a lipid production substrate for cold‐adapted microorganisms including Y. lipolytica yeast. Besides, lipid production potential of Y. lipolytica under non‐sterile culture conditions was investigated for the first time in this study. © 2015 Society of Chemical Industry and John Wiley & Sons, Ltd

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Purification and characterization of extracellular β-galactosidase from the psychrotolerant yeast Guehomyces pullulans 17-1 isolated from sea sediment in Antarctica

Cited by 35 publications

References 26 publications

Use of Yeasts as Probiotics in Fish Aquaculture

Use of Yeasts as Probiotics in Fish Aquaculture

Production, recovery and purification of a recombinant β-galactosidase by expanded bed anion exchange adsorption

Microbial lipid production by cold‐adapted oleaginous yeast Yarrowia lipolytica B9 in non‐sterile whey medium

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