Purification and Characterization of Ferredoxin–Sulfite Reductase from Turnip (Brassica rapa) Leaves and Comparison of Properties with Ferredoxin–Sulfite Reductase from Turnip Roots

Bioinspired synthesis, characterization and antifungal activity of enzyme-mediated gold nanoparticles using a fungal oxidoreductase

“…The purity and apparent molecular weight of fungal sulfite oxidoreductase was checked by SDS-PAGE using protein marker as standard [34,35]. The molecular weight of purified fungal sulfite oxidoreductase was determined by size barring chromatography on Sephacryl S-300 which was balanced with 0.2 M sodium phosphate buffer (pH 7.2) containing 1 mM EDTA (pH 7.2).…”

“…To 1.4 ml reaction mixture [0.2 M phosphate buffer (pH 7.2) including 1.0 mM EDTA, 0.15 mM NADPH, 1.0 mM of newly prepared sodium sulfite], 0.1 ml of oxidoreductase was added and incubated at different temperatures (25,30,35,40,45,50,55, 60 °C) for 10 min. The activity was defined from primary speed and corrected for the slow oxidation of NADPH in the absence of sulfite [31].…”

“…The activity was defined from primary speed and corrected for the slow oxidation of NADPH in the absence of sulfite [31]. Purified Fungal NADPH-sulfite oxidoreductase in 0.2 M phosphate buffer (pH 7.2) including 1.0 mM EDTA, 0.15 mM NADPH and 1.0 mM sodium sulfite was incubated at various temperatures (25,30,35,40,45,50,55, 60 °C). After 10 min incubation, the activity was measured according to Yoshimoto and Sato [31,32].…”

Genetic Engineering of Plants to Enhance Selenium Phytoremediation

“…Several genes and cDNAs encoding sulfate transporters have been identified from different plant species, and individual transporters are expressed in specific plant tissues and show different affinities for sulfate Smith et al, 1995Smith et al, , 1997Takahashi et al, 1996Takahashi et al, , 1997Bolchi et al, 1999;Heiss et al, 1999;Saito, 2000). The presence of a SeO 4 2-specific transporter in Se hyperaccumulators has also been suggested based on the high Se:S ratio found in their tissues, but as of yet none has been identified definitively (Rosenfield and Beath, 1964).…”

“…Perhaps it is possible to identify a common transcription factor that can be used to modulate expression of coordinately regulated genes in the S/Se assimilation pathway. In this pathway, the expression of at least three genes, sulfate permease, ATP sulfurylase, and APS reductase, is regulated similarly (Schmidt, 1986;Clarkson et al, 1993;Lappartient et al, 1996;Setya et al, 1996;Hell, 1997;Takahashi et al, 1997;Bolchi et al, 1999). These genes are upregulated under conditions of S starvation and downregulated under S-sufficient conditions in a number of plant species.…”